Analysis of antiseptic resistance in yeast.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Iwate Medical University
• Principal Investigator: OHASHI Kazuaki Iwate Medical University, 薬学部, 准教授 (70344679)
• Project Period (FY): 2008 – 2010
• Keywords: 薬学 / 酵母

Research Abstract

So far I clarified overexpression of SGE1 in yeast confers resistance to benzalkonium chloride and chlorhexidine. Effect of overexpression or deletion of AZR1, VBA3 and VBA5 to antiseptic sensitivity in yeast was examined. Overexpression of these genes did not confer resistance to benzalkonium chloride nor chlorhexidine in yeast. Deletion of SGE1, AZR1, VBA3 and VBA5 did not affect sensitivity to benzalkonium chloride nor chlorhexidine. Yeast deletion mutants showing resistance or sensitive to benzalkkonium chloride were screened to obtain numerous candidates.

Research Products

• [Journal Article] Proposal to conserve the name Codariocalyx (Leguminosae/Fabaceae) with that spelling. (2011)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: Taxon 60 Pages: 239
• [Journal Article] The Correct Scientific Name of "Shirobana-hagi", the Oldest White-Flowered Race of Lespedeza (Leguminosae) in Japan). (2011)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 86 Pages: 36-41
• [Journal Article] Proposal to modify Article 37 Example 4. (2010)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: Taxon 59 Pages: 1612
• [Journal Article] Identity of Lespedeza bicolor Turcz. f. albiflora Matsum. (Leguminosae). (2010)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 85 Pages: 322-330
• [Journal Article] Amendments in Codariocalyx (Leguminosae). (2010)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 85 Pages: 238-240
• [Journal Article] Proposals to add two new recommendations in Recommendation 37A. (2010)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: Taxon 59 Pages: 987
• [Journal Article] Entada (Leguminosae subfam. Mimosoideae) of Taiwan. (2010)
  • Author(s): Hiroyoshi Ohashi, Tseng-chieng Huang, Kazuaki Ohashi
  • Journal Title: Taiwania 55 Pages: 43-53
• [Journal Article] New Combinations of Melanthera (Asteraceae) in Japan and Taiwan. (2010)
  • Author(s): Kazuaki Ohashi, Hiroyoshi Ohashi
  • Journal Title: J.Jpn.Bot. 85 Pages: 59-63
• [Journal Article] Correction of Author names in East Asian Selliguea (Polypodiaceae) in Japan. (2010)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 85 Pages: 46
• [Journal Article] Three white-flowered forms of Lespedeza (Leguminosae) in Japan. (2009)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 84 Pages: 359-360
• [Journal Article] New combinations of Selliguea (Polypodiaceae) in Japan, Korea and Taiwan. (2009)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 84 Pages: 305-309
• [Journal Article] A revision of Lespedeza subgenus Macrolespedeza (Leguminosae) in China. (2009)
  • Author(s): Hiroyoshi Ohashi, Tomoyuki Nemoto, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 84 Pages: 197-223
• [Journal Article] A revision of Lespedeza subgenus Lespedeza (Leguminosae) of China. (2009)
  • Author(s): Hiroyoshi Ohashi, Tomoyuki Nemoto, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 84 Pages: 143-166
• [Journal Article] Identity of Lespedeza anthobotrya Ricker and L. bracteolata Ricker (Leguminosae). (2009)
  • Author(s): Hiroyoshi Ohashi, Tomoyuki Nemoto, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 84 Pages: 185-188
• [Journal Article] Normal formation of a subset of intestinal granules in Caenorhabditis elegans requires ATP-binding cassette transporters HAF-4 and HAF-9, which are highly homologous to human lysosomal peptide transporter TAP-like. (2009)
  • Author(s): Hiromi Kawai, Takahiro Tanji, Hirohisa Shiraishi, Mitsuo Yamada, Ryoko Iijima, Takao Inoue, Yasuko Kezuka, Kazuaki Ohashi, Yasuo Yoshida, Koujiro Tohyama, Keiko Gengyo-Ando, Shohei Mitani, Hiroyuki Arai, Ayako Ohashi-Kobayashi, Masatomo Maeda
  • Journal Title: Mol.Biol.Cell 20 Pages: 2979-2990
• [Journal Article] Functional dissection of transmembrane domains of human TAP-like (ABCB9) Biochem. (2008)
  • Author(s): Aya Kamakura, Yasuyuki Fujimoto, Yu Motohashi, Kazuaki Ohashi, Ayako Ohashi-Kobayashi, Masatomo Maeda
  • Journal Title: Biophys.Res.Commun. 377 Pages: 847-851
• [Journal Article] Potentilla hebiiichigo Yonek. & H. Ohashi (Rosaceae) and its distribution (2008)
  • Author(s): Koji Yonekura, Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 83 Pages: 301-305
• [Journal Article] Identity of Duchesnea indica var. arbicarpa Y. N. Lee (Rosaceae) from Korea (2008)
  • Author(s): Koji Yonekura, Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 83 Pages: 314
• [Journal Article] Typification of Scutellaria guilielmii A. Gray (Lamiaceae) (2008)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 83 Pages: 253-255
• [Journal Article] Lectotypification of Taiwania cryptomerioides Hayata (Cupressaceae) (2008)
  • Author(s): Hiroyoshi Ohashi, Kazuaki Ohashi
  • Journal Title: J.Jpn.Bot. 83 Pages: 177-184
• [Presentation] Contribution of heparan sulfate fine structure to insulin secretion. (2010)
  • Author(s): Iwao Takahashi, Kazuaki Ohashi, Nausheen J. Shervani, Koji Nata
  • Organizer: 第33回日本分子生物学会年会/第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] (Regenerating gene) family proteins in type 1 and type 2 diabetes. (2010)
  • Author(s): Nausheen J. Shervani, Iwao Takahashi, Kazuaki Ohashi, Naoya Noguchi, Hiroshi Okamoto, Shin Takazawa, Akira Sugawara, Koji Nata
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-29
• [Presentation] Sera from Japanese diabetes patient show autoimmunity to REG family antigens. (2009)
  • Author(s): Nausheen J. Shervani, Koji Nata, Naoya Noguchi, Iwao Takahashi, Takayuki Ikeda, Kazuaki Ohashi, Takeo Yoshikawa, Akira Uruno, Shin Takasawa, Hiroshi Okamoto, Akira Sugawara
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Remarks] ホームページ等