2010 Fiscal Year Final Research Report
ヒト胎児肝細胞におけるCYP3A分子種の発現変動要因の解明
| Project/Area Number |
20590142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Shinshu University |
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Principal Investigator |
MATSUNAGA Tamihide Shinshu University, 大学院・薬学研究科, 教授 (40209581)
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| Co-Investigator(Kenkyū-buntansha) |
OHMORI Shigeru 信州大学, 医学部・附属病院, 教授 (70169069)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 薬物動態 / 代謝学 |
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| Research Abstract |
In HFL cells overexpressed PXR, RIF-mediated CYP3A4 induction was insufficient compared with HepG2 cells. Lower expression of HNF4α and PGC1α might impair RIF-mediated CYP3A4 induction in HFL cells. The expression levels of CYP3A4, CYP3A7 and VEGF mRNAs were significantly increased by DFO. The expression levels of CYP3A4 and CYP3A7 mRNAs in HFL cells were reduced by hypoxia (3% O_2), although the expression level of VEGF mRNA was enhanced. These results suggest that character of immature liver cells such as HFL cells with regard to CYP expression is different from that of highly differentiated human hepatocytes.
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