Analysis for molecular mechanism of contactin associated protein on intractable epilepsy

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kagoshima University
• Principal Investigator: TAKEDA Yasuo Kagoshima University, 医学部・歯学部附属病院, 准教授 (60245462)
• Co-Investigator(Kenkyū-buntansha): YAMADA Katushi 鹿児島大学, 医学部・歯学部附属病院, 教授 (00037491)
• Research Collaborator: XIAO Zhi-cheng Monash大学, 医学部, 教授,オーストラリア ; FUTAGAWA Toshitaka 鹿児島大学, 医学部・歯学部附属病院, 薬剤師 ; OISO Shigeru 鹿児島大学, 医学部・歯学部附属病院, 薬剤師 ; MASUDA Shogo 鹿児島大学, 医学部・歯学部附属病院, 薬剤師
• Project Period (FY): 2008 – 2010
• Keywords: 臨床薬学 / 難治性てんかん / コンタクチン関連蛋白質 / トランスゴルジ輸送系

Research Abstract

To investigate the molecular mechanism for trafficking of Contactin-associated protein (Caspr) 2 to the cem membrane, we identified carboxypeptidase E (CPE) as a binding partner of Caspr2. Both Caspr2- and CPE-like immunoreactivities (IR) were found to co-localize in the apical dendrites and cell bodies of rat cortical neurons. In subcellular localization analysis, Caspr2- and CPE-like IRs were comigrated in the fractions of Golgi/ER. When Caspr2 cDNA was transfected into COS-7 cells with or without CPE cDNA, Caspr2 localized only in Golgi/ER without CPE, but localized in both Golgi/ER and plasma membrane with CPE. Our data suggest that CPE may be a key molecule to regulate Caspr2 trafficking to the cell membrane.

Research Products

• [Journal Article] Higher linezolid exposure and higher frequency of thrombocytopenia in patients with renal dysfunction. (2010)
  • Author(s): Matsumoto K., Takeshita, A., Ikawa, K., Shigemi, A., Yaji, K., Shimodozono Y., Morikawa N., Takeda Y., Yamada K.
  • Journal Title: Int.J.Antimicrob.Agents 36 Pages: 179-181
  • Peer Reviewed
• [Journal Article] Synaptic formation in subsets of glutamatergic terminals in the mouse hippocampal formation is affected by a deficiency in the neural cell recognition molecule NB-3. (2010)
  • Author(s): Sakurai, K., Toyoshima, M., Takeda Y., Shimoda, Y., Watanabe K.
  • Journal Title: Neurosci.Lett. 473 Pages: 102-106
  • Peer Reviewed
• [Journal Article] Major vault protein forms complexes with hypoxia-inducible factor (HIF)-1α and reduces HIF-1α level in ACHN human renal adenocarcinoma cells. (2010)
  • Author(s): Iwashita, K., Ikeda, R., Takeda Y., Sumizawa, T., Furukawa, T., Yamaguchi, T., Akiyama, S., Yamada K.
  • Journal Title: Cancer Sci. 101 Pages: 920-926
  • Peer Reviewed
• [Journal Article] Determination of teicoplanin through concentration target and appropriate total dose during the first 3 days : a retrospective study in patients with MRSA infections. (2010)
  • Author(s): Matsumoto K., Kanazawa, N., Ikawa, K., Fukamizu T., Shigemi, A., Yaji, K., Shimodozono Y., Morikawa N., Takeda Y., Yamada K.
  • Journal Title: J.Infect.Chemother. 16 Pages: 193-199
  • Peer Reviewed
• [Journal Article] Safety evaluation of Black Rice Vinegar (Kurosu) from a Jar on food-drug interaction : 30-day ingestion study on expressions of drug metabolism enzyme and transporters in rats. (2010)
  • Author(s): Shibayama, Y., Nagano, M., Fujii, A., Takeda Y., Yamada K.
  • Journal Title: J.Health Sci. 56 Pages: 712-716
  • Peer Reviewed
• [Journal Article] Contactin-associated protein (Caspr) 2 interacts with carboxypeptidase E in the central nervous system. (2009)
  • Author(s): Oiso, S., Takeda Y, Futagawa, T., Miura, T., Kuchiiwa, S., Nishida, K., Ikeda, R., Kariyazono, H., Watanabe, K., Yamada K.
  • Journal Title: J.Noeurochem. 109 Pages: 158-167
  • Peer Reviewed
• [Journal Article] Deficiency of Neural recognition molecule NB-2 affects the development of glutamatergic auditory pathways from the ventral cochlear nucleus to superior olivary complex in mouse. (2009)
  • Author(s): Toyoshima, M., Sakurai, K., Shimazaki, K., Takeda Y., Shimoda, Y., Watanabe, K.
  • Journal Title: Developmental Biol. 336 Pages: 192-220
  • Peer Reviewed
• [Journal Article] Contribution of the neural cell recognition molecule NB-3 to synapse formation between parallel fibers and Purkinje cells in mouse. (2009)
  • Author(s): Sakurai, K., Ueda, H., Matsubara, K., Takeda Y., Karagogeos, D., Shimoda, Y., Watanabe, K.
  • Journal Title: Developmental Neurobiol. 69 Pages: 811-824
  • Peer Reviewed
• [Journal Article] Correlation between voriconazole trough plasma concentration and hepatotoxicity in patients with different CYP2C19 genotypes. (2009)
  • Author(s): Matsumoto K., Ikawa, K., Abematsu, K., Fukunaga N., Nishida, K., Fukamizu T., Shimodozono Y., Morikawa N., Takeda Y., Yamada K.
  • Journal Title: Int.J.Antimicrob.Angents, 34 Pages: 91-94
  • Peer Reviewed
• [Journal Article] Preferential localization of neural cell recognition molecule NB-2 in developing glutamatergic neurons in the rat auditory brainstem. (2009)
  • Author(s): Toyoshima, M., Sakurai, K., Shimazaki, K., Takeda Y, Nakamoto, M., Serizawa, S., Shimoda, Y., Watanabe, K.
  • Journal Title: J.Comp.Neurol. 531 Pages: 349-362
  • Peer Reviewed
• [Journal Article] Effect of methotrexate treatment on expression levels of organic anion transporter polypeptide 2, P-glycoprotein and bile salt export pump in rats. (2009)
  • Author(s): Shibayama, Y., Takeda Y., Yamada K.
  • Journal Title: Biol.Pharm.Bull. 32 Pages: 493-496
  • Peer Reviewed
• [Journal Article] シクロスポリンおよびタクロリムスのTDM研究支援ソフトウェアの開発 (2009)
  • Author(s): 金子生、猪川和朗、福原慶、森川則文、福永直子、深水知英、松元一明、下堂薗権洋、武田泰生、山田勝士
  • Journal Title: TDM研究 26巻 Pages: 52-58
  • Peer Reviewed
• [Journal Article] A TAG1-APP signaling pathway through Fe65 negatively modulates neurogenesis. (2008)
  • Author(s): Ma, Q.-H., Futagawa, T., Yang, W.-L., Jiang, X.-D., Zeng L., Takeda Y., Xu, R.-X., Bagnard, D., Schachner, M., Furley, A.J., Karagogeos, D., Watanabe, K., Dawe, G.S., Xiao, Z.-C.
  • Journal Title: Nature Cell Biol. 10 Pages: 283-294
  • Peer Reviewed
• [Journal Article] Neural recognition molecules CHL1 and NB-3 regulate apical dendrite orientation in the neocortex via PTPα. (2008)
  • Author(s): Ye, H., Tan, Y. L.J., Ponniah, S., Takeda Y., Wang, S.-Q., Schachner, M., Watanabe, K., Pallen, C.J., Xiao, Z.-C.
  • Journal Title: EMBO J. 27 Pages: 188-200
  • Peer Reviewed
• [Journal Article] Up-regulation of matrix metalloproteinase-3 in the dorsal root ganglion of rats with paclitaxel-induced neuropathy. (2008)
  • Author(s): Nishida, K., Kuchiiwa, S., Oiso, S., Futagawa, T., Masuda, S., Takeda Y., Yamada K.
  • Journal Title: Cancer Sci. 99 Pages: 1618-1625
  • Peer Reviewed
• [Journal Article] Stability of high-dose methylcobalamin injection. (2008)
  • Author(s): Kusadome, C., Shibayama, Y., Nishi, Y., Takeda Y., Yamada K.
  • Journal Title: J.Applied Ther.Res. 6 Pages: 15-18
  • Peer Reviewed
• [Journal Article] Thymidine phosphorylase inhibits the expression of proapoptotic protein BNIP3. (2008)
  • Author(s): Ikeda, R., Tajitsu, Y., Iwashita, K., Che, X.F., Yoshida, K., Ushiyama, M., Furukawa, T., Komatsu, M., Yamaguchi, T., Shibayama, Y., Yamamoto, M., Zhao, H.Y., Arima, J., Takeda Y., Akiyama, S., Yamada K.
  • Journal Title: Biochem.Biophys.Res.Commun. 370 Pages: 220-224
  • Peer Reviewed
• [Presentation] (2010)
  • Author(s): 山口沙織、益田将吾、二川俊隆、鬼丸貴裕、末廣淑子、武田泰生、山田勝士
  • Organizer: 第27回日本薬学会九州支部大会
  • Place of Presentation: 長崎
  • Year and Date: 2010-12-11
• [Presentation] (2010)
  • Author(s): Futagawa T., Onimaru T., Chen, Z. C., Masuda S., Suehiro Y., Yamaguchi S., Xiao, Z. C., Takeda Y., Yamada K.
  • Organizer: The 20^<th> Japan-Korea Joint Seminar on Pharmacology
  • Place of Presentation: Kagoshima
  • Year and Date: 2010-11-26
• [Presentation] (2010)
  • Author(s): 末廣淑子、二川俊隆、益田将吾、鬼丸貴裕、山口沙織、武田泰生、山田勝士
  • Organizer: 第63回日本薬理学会西南部会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-11-26
• [Presentation] (2010)
  • Author(s): 武田泰生
  • Organizer: 第20 回日本医療薬学会
  • Place of Presentation: 千葉
  • Year and Date: 2010-11-13
• [Presentation] (2010)
  • Author(s): Saito, S., Arima, J., Shimodozono Y., Takeda Y., Yamada K.
  • Organizer: The 23^<rd> Federation of Asian Pharmaceutical Associations Congress
  • Place of Presentation: Taiwan
  • Year and Date: 2010-11-06
• [Presentation] (2010)
  • Author(s): Takeda Y., Matsumoto, K., Takeshita, A., Ikawa, K., Morikawa, N., Yamada K.
  • Organizer: 日本薬物動態学会第25回年会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-08
• [Presentation] (2010)
  • Author(s): Ushiyama, M., Ikeda R., Miyawaki, A., Yamaguchi, T., Tazitsu Y., Nishizawa, Y., Mataki, H., Nakamura, N., Takeda Y., Yamada K.
  • Organizer: 70^<th> International Congress of FIP
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2010-08-30
• [Presentation] (2009)
  • Author(s): 藤井寛樹、二川俊隆、益田将吾、石原義久、鬼丸貴裕、末廣淑子、山口沙織、武田泰生、山田勝士
  • Organizer: 第26回日本薬学会九州支部大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-13
• [Presentation] (2009)
  • Author(s): 武田泰生
  • Organizer: 2009 China-Japan Pharmacists' International Forum
  • Place of Presentation: Beijing, China
  • Year and Date: 2009-11-14
• [Presentation] (2009)
  • Author(s): 武田泰生, 西田健太朗, 山田勝士
  • Organizer: Asian Federation for Pharmaceutical Sciences
  • Place of Presentation: Fukuoka
  • Year and Date: 2009-10-16
• [Presentation] (2009)
  • Author(s): Kondo, T., Matsumoto, K., Tanaka, S., Yamaguchi, T., Nishi, S., Ueno K., Ogura, T., Takeda Y., Yamada K.
  • Organizer: 69^<th> International Congress of FIP
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2009-09-05
• [Presentation] (2009)
  • Author(s): 武田泰生、益田将吾、二川俊隆、西田健太朗、田畑祥、藤井寛樹、山田勝士
  • Organizer: 医療薬学フォーラム2009
  • Place of Presentation: 京都
  • Year and Date: 2009-07-11
• [Presentation] (2009)
  • Author(s): 田實裕介、池田龍二、岩下建一、武田泰生、山田勝士
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-17
• [Presentation] (2008)
  • Author(s): 重永明恵、屋地慶子、上野恵、下堂薗権洋、武田泰生、山田勝士
  • Organizer: 医療薬学フォーラム2008年7月12日
  • Place of Presentation: 東京
  • Year and Date: 20080000
• [Presentation] (2008)
  • Author(s): Ikeda R., Takeda Y., Akiyama, S., Yamada K.
  • Organizer: 22^<nd> Federation of Asian Pharmaceutical Association Congress
  • Place of Presentation: Singapore
  • Year and Date: 2008-11-08
• [Presentation] (2008)
  • Author(s): 武田泰生,西田健太朗, 山田勝士
  • Organizer: The 19^<th> Korea-Japan Joint Seminar on Pharmacology
  • Place of Presentation: Busan
  • Year and Date: 2008-11-07
• [Presentation] (2008)
  • Author(s): 武田泰生
  • Organizer: 68^<th> International Congress of FIP
  • Place of Presentation: Basel, Switzerland
  • Year and Date: 2008-09-03
• [Book] カッツング薬理学【原著10版】 (2009)
  • Author(s): 武田泰生、他
  • Total Pages: 36-50,51-64,493-512,513-527
  • Publisher: 丸善出版
• [Book] パートナー形態機能学 (2008)
  • Author(s): 武田泰生、他
  • Total Pages: 95-117
  • Publisher: 南山堂出版
• [Book] 病気と薬パーフェクトBOOK 2008 (2008)
  • Author(s): 武田泰生、他
  • Total Pages: 496-497,501,504,508-509,515-517,520-521,530-532,536-537,540-541,547-548,554
  • Publisher: 南山堂出版