2010 Fiscal Year Final Research Report
Analysis of lipid-based signal transduction
| Project/Area Number |
20590189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
FUJITA Akikazu Nagoya University, 医学系研究科, 准教授 (60282232)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Toyoshi 名古屋大学, 大学院・医学系研究科, 教授 (50115929)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 電子顕微鏡 / 細胞内情報伝達 / カベオラ |
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| Research Abstract |
It has been known that inositolphospholipid PI(4,5)P_2, whose pool is mainly localized in the plasma membrane, has some cellular functions, such as signal transduction, endocytosis, cytoskeleton regulation and so on. In this study, we show that, using freeze-fracture replica labeling method, the time course of reduction in the PI(4,5)P_2 level in the plasma membrane is different in each area of the undifferential plasma membrane, caveola, coated pit when cells are stimulated with angiotensin II or Ca^<2+> ionophore. These results indicate that PI(4,5)P_2 is compartmentalized in the plasma membrane, resulting in changing the level of PI(4,5)P_2 in the limited area.
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