Molecular Cell Biological Analysis of Nuclear Condensation in Apoptosis

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Kawasaki Medical School
• Principal Investigator: TONE Shigenobu Kawasaki Medical School, 医学部, 准教授 (70211399)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Takeaki 川崎医科大学, 医学部, 助教 (20398431)
• Project Period (FY): 2008 – 2010
• Keywords: 分子形態学 / アポトーシス

Research Abstract

This study characterized the condensation factor(s)which induced the ring condensation in nuclei during apoptosis using cell-free apoptosis system. Neither DNase, caspases nor JNK kinase would be involved in ring condensation.

Research Products

• [Journal Article] Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state. (2011)
  • Author(s): Jrad-Lamine A, et al
  • Journal Title: Journal of Biological Chemistry 286 Pages: 8030-8042
  • Peer Reviewed
• [Journal Article] Hepatitis C virus and disrupted interferon signaling promote lymphoproliferation via type II CD95 and interleukins (2009)
  • Author(s): Machida K, et al
  • Journal Title: Gastroenterology 137 Pages: 285-96
  • Peer Reviewed
• [Journal Article] Imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor. (2009)
  • Author(s): Sugimoto K, Tone S
  • Journal Title: Methods in Molecular Biology 591 Pages: 135-146
• [Journal Article] Possible involvement of caspase-6 and -7 but not caspase-3 in the regulation of hypoxia-induced apoptosis in tube-forming endothelial cells. (2009)
  • Author(s): Eguchi R, Tone S, et al
  • Journal Title: Exp.Cell Res 315 Pages: 327-35
  • Peer Reviewed
• [Presentation] Proapoptotic Role of DNA-PK System in DT40 Apoptosis Induced by X-ray (2010)
  • Author(s): Tone S, Kuribayashi F, Takata M, Ishiai M
  • Organizer: UV or Hydrogen Peroxide American Society for Cell Biology Annual Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 20101200
• [Presentation] Ku70 and DNA-PKcs are essential in DT40 apoptosis induced by X-ray (2009)
  • Author(s): Tone S, Tanda K Takata M Ishiai M
  • Organizer: UV or H2O2 Keystone Symposia Conference "Cell Death Pathways"
  • Place of Presentation: Whistler, Canada
  • Year and Date: 20090300
• [Presentation] Dynamic aspects of nuclear condensation revealed by time-lapse imaging, biochemical and electron microscopy analysis of cell-free apoptosis. (2007)
  • Author(s): Tone S, Tanda K Sugimoto K Suda T Uehira K, Kanouchi H Samejima K Minatogawa Y, Earnshaw W C
  • Organizer: EMBO Conference Series on Nuclear Structure & Dynamics.
  • Place of Presentation: Montpelier, France
  • Year and Date: 20070900