2010 Fiscal Year Final Research Report
The regulation mechanisms of subcellular localization of ion channels in developing neurons.
| Project/Area Number |
20590217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Saitama Medical University |
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Principal Investigator |
NAKAHIRA Kensuke Saitama Medical University, 医学部, 講師 (10260043)
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| Project Period (FY) |
2008 – 2010
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| Keywords | シナプス伝達 / 電位依存性Kチャネル / 小脳顆粒細胞 / 培養神経細胞 |
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| Research Abstract |
Kv4.2, an A-type K+ channel, changed its subcellular localization from soma to the dendrites and synapses during synaptogenesis. In order to investigate the mechanisms of this regulation, we used EGFP-tagged-fusion channels. The fusion proteins were found mainly in ER when cDNA was transfected in culture cells. The Kv4.2 binding proteins, such as KChIP1, enhanced the surface expression of the channels by promoting translocation from ER to Golgi body.
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