Relationship of circadian rhythm with cycle control

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590234
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Nagoya City University
• Principal Investigator: ISOBE Yoshiaki Nagoya City University, 大学院・医学研究科, 助教 (70094357)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Makoto 名古屋市立大学, 大学院・医学研究科, 教授 (40217774) ; HIDA Hideki 名古屋市立大学, 大学院・医学研究科, 教授 (00305525)
• Project Period (FY): 2008 – 2010
• Keywords: 概日リズム / 細胞周期 / ES細胞 / 分化 / 時計遺伝子

Research Abstract

Two self-oscillating systems are running in vertebrate organ that are circadian rhythm and cell cycle. Proliferating neurosphere was prepared from the dispersed cell culture of preoptic neuroepitherium in E14 rat fetus brain. After the second passage, the cells were collected 6 hr intervals at 9:00, 15:00, 21:00 and 03:00. Proportion of G1cells at 9:00 was smaller than that at 21:00. Per2 and p27 mRNAs showed similar circadian rhythms, which peaked at 21:00. From the results, cell cycle is tightly controlled by the component of circadian rhythm.

Research Products

• [Journal Article] Circadian rhythm of enolase in suprachiasmatic nucleus depends on the mitochondrial function. (2011)
  • Author(s): Isobe, Y., Hida, H., Nishino, H.
  • Journal Title: J.Neurosci.Res. 89 Pages: 936-944
  • Peer Reviewed
• [Journal Article] Circadian rhythm of metabolic oscillation in suprachiasmatic nucleus depends on the mitochondrial oxidation state, reflected by cytochrome C oxidase and LDH. (2011)
  • Author(s): Isobe, Y., Hida, H., Nishino, H.
  • Journal Title: J.Neurosci.Res. 89 Pages: 929-935
  • Peer Reviewed
• [Journal Article] Diethylnitrosamine-induced hepatic lesions are greater in rats maintained under a light-dark cycle than under constant light, related to the locomotor activity rhythm. (2008)
  • Author(s): Isobe, Y., Fukamachi, K., Hida, H., Tsuda, H., Nishino, H.
  • Journal Title: Asian Pac.J.Cancer Prev. 9 Pages: 619-624
  • Peer Reviewed
• [Journal Article] Enhanced neurogenesis from neural progenitor cells with G1/S-phase cell cycle arrest is mediated by transforming growth factor betal. (2008)
  • Author(s): Misumi, S., Kim, TS., Jung, CG., Masuda, T., Urakawa, S., Isobe, Y., Furuyama, F., Nishino, H., Hida, H.
  • Journal Title: Eur J Neurosci. 28 Pages: 1049-1059
  • Peer Reviewed
• [Journal Article] Increase in dopaminergic neurons from mouse embryonic stem cell-derived neural progenitor/stem cells is mediated by hypoxia inducible factor-1alpha. (2008)
  • Author(s): Kim, TS., Misumi, S., Jung, CG., Masuda, T., Isobe, Y., Furuyama, F., Nishino, H., Hida, H.
  • Journal Title: J Neurosci Res. 86 Pages: 2353-2362
  • Peer Reviewed
• [Presentation] ES細胞のサーカディアンリズムと細胞周期との関係 (2010)
  • Author(s): 磯部芳明
  • Organizer: 第33日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2010-09-02
• [Presentation] Diethylnitrosamineの明暗周期下投与時の肝毒性は24時間に2峰性をしめす (2010)
  • Author(s): 磯部芳明、津田洋幸
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール
  • Year and Date: 2010-05-19
• [Presentation] Diethylnitrosamine induced hepatic carcinogenicity was cruel kept under the light-dark cycle than constant dim light and different by an injection-time of day in rats. (2008)
  • Author(s): Isobe, Y., Tsuda Y.
  • Organizer: 18th International Congress of Biometeorology (ICB2008)
  • Place of Presentation: Tokyo Tower Hall Funabori
  • Year and Date: 20080922-20080926
• [Presentation] Diethylnitrosamineの明暗周期下での投与時刻による、自発活動リズムと肝ガン発生の差異(1P-G-116) (2008)
  • Author(s): 磯部芳明、津田洋幸
  • Organizer: 第85 回日本生理学会大会
  • Place of Presentation: 京王プラザホテル東京
  • Year and Date: 2008-03-25