2010 Fiscal Year Final Research Report
In silico searching for biologically active peptides with a C-terminal amide based on h uman genome DNA structure
| Project/Area Number |
20590248
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Chiba University |
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Principal Investigator |
KIMURA Sadao Chiba University, 大学院・医学研究院, 教授 (40134225)
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| Co-Investigator(Kenkyū-buntansha) |
KASUYA Yoshitoshi 千葉大学, 大学院・医学研究院, 准教授 (70221877)
NISHIYAMA Mariko 千葉大学, 大学院・医学研究院, 助教 (00092081)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 生理活性ペプチド / 生合成経路 / オーファン受容体 / G蛋白質共役型受容体 / GPCR / in silico |
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| Research Abstract |
We have developed the computer program soft to predict the bioactive peptides based on the amino acid sequences of the precursor protein. The empirical biosynthetic rules of bioactive peptide from their precursor proteins were incorporated into the program, namely, precursor proteins contain a signal peptide and 50-300 amino acid residues, the lengths of the active peptides are 6-60 amino acid residues with 0～2 cystine (disulfide bond), and dibasic amino acid pairs at the cleavage sites. From the data bank of human genome structure (33,119 proteins), we predicted 352 candidates of bioactive peptides with a C-terminal amide. About 200 peptides of them were chemically synthesized and analyzed whether they are ligands to orphan G protein-coupled receptors or not.
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[Journal Article] N-type calcium channel in the pathogenesis of experimental autoimmune encephalomyelitis2010
Author(s)
Tokuhara N, Namiki K, Uesugi M, Miyamoto C, Ohgoh M, Ido K, Yoshinaga T, Yamauchi T, Kuromitsu J, Kimura S, Miyamoto N, Kasuya Y
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Journal Title
J.Biol.Chem. 285(43)
Pages: 33294-33306
Peer Reviewed
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