2010 Fiscal Year Final Research Report
Involvement of membrane-associated prostaglandin E synthase-1 in neovascularization.
| Project/Area Number |
20590259
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
NARABA Hiroaki Iwate Medical University, 薬学部, 准教授 (90296517)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 遺伝子発現 / 転写調節 / プロスタグランジン / シクロオキシゲナーゼ / 炎症性病態 / 新生血管 |
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| Research Abstract |
Membrane-associated prostaglandin E_2 synthase (mPGES1) is an inducible terminal enzyme in the biosynthetic pathway for prostaglandin E_2, which participates in many biological processes such as neovascularization. We have already shown that Egr-1 is a key transcription factor in regulating the inducible expression of mPGES. In this study I have investigated the role of NAB2, a specific corepressor of EGR-1, to down-regulate growth factor-induced mPGES expression in inflammatory cells and to inhibit neovascularization. NAB2 was rapidly induced by increased levels of Egr-1 in vitro and in vivo model of neovascularization. Moreover, it was observed that the induction of mPGES1 gene expression and production of PGE_2 by treatment of siRNA specific for NAB2. These results suggest that NAB2 is involved in the regulation of the expression of mPGES1, and it may serve as a key molecule in the development and progression of neovascularization.
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