2010 Fiscal Year Final Research Report
Regulation of expression of vascular cation channels by transcriptional and epigenetical modulation
| Project/Area Number |
20590263
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
MURAKI Katsuhiko Aichi Gakuin University, 薬学部, 教授 (20254310)
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| Co-Investigator(Kenkyū-buntansha) |
HATANO Noriyuki 愛知学院大学, 薬学部, 講師 (50454319)
ITOH Yuka 名古屋市立大学, 大学院・薬学研究科, 助教 (40454326)
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| Project Period (FY) |
2008 – 2010
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| Keywords | カチオンチャネル / エピジェネティクス / 転写 |
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| Research Abstract |
Change of ion channel expression by transcriptional and epigenetical modulation plays a pivotal role in development of human diseases. We thus investigated regulation of expression of a non-selective cation channel, nicotinic ACh receptor α7 (nAChRα7) in human synovial fibroblasts (HSFs). Analysis of expression of nAChRα7 mRNA transcripts between HSFs with or without rheumatoid arthritis (RA) revealed that nAChRα7 was lower in normal HSFs. Promoter of nAChRα7 gene was methylated only in normal HSFs. The expression of nAChRα7 mRNA transcripts was increased when the methylation was inhibited by treatment of normal HSFs with 5-aza-CdR. In HFSs with RA, expression of nAChRα7 is increased due to inhibition of methylation of nAChRα7 gene.
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