2010 Fiscal Year Final Research Report
Analysis of physiological function of calcium transporters in epithelial cells and its pathological mechanisms
| Project/Area Number |
20590270
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Fukuoka University |
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Principal Investigator |
IWAMOTO Takahiro Fukuoka University, 医学部, 教授 (20300973)
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| Co-Investigator(Kenkyū-buntansha) |
IYODA Takuya 福岡大学, 医学部, 助教 (80465715)
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| Project Period (FY) |
2008 – 2010
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| Keywords | イオン輸送 / 上皮細胞 / 腎機能 / 血管機能 / 薬理学 |
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| Research Abstract |
We studied the physiological function of Na^+/Ca^<2+> exchanger (NCX) in epithelial cells (renal tubular epithelium, vascular endothelium) and its pathological role, using genetically altered mice and NCX inhibitors. Suppression of NCX1 function (induced by heterozygous or isoform-specific inhibitor) mitigated the renal tubular injury after renal ischemic-reperfusion, whereas suppression of NCX2 function deteriorated the renal tubular injury. We also screened the protein associated with the intracellular domain of NCX1 (as a bait) by using yeast two-hybrid system, and then identified the new adapter protein (scaffold protein) bound to NCX1.
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[Journal Article] The pump, the exchanger, and endogenous ouabain : signaling mechanisms that link salt retention to hypertension2009
Author(s)
Blaustein MP, Zhang J, Chen L, Song H, Raina H, Kinsey SP, Izuka M, Iwamoto T, Kotlikoff MI, Lingrel JB, Philipson KD, Wier WG, Hamlyn JM
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Journal Title
Hypertension 53
Pages: 291-298
Peer Reviewed
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[Journal Article] Crystallization and preliminary X-ray crystallographic analysis of Ca^<2+>-free primary Ca^<2+>-sensor Na^+/Ca^<2+> exchanger2008
Author(s)
Mima M, Kawai C, Paku K, Tomoo K, Tomoo K, Ishida T, Sugiyama S, Matsumura H, Kitatani Yoshikawa HY, Maki S, Adachi H, Takano K, Murakami S, Inoue T, Mori Y, Kita S, Iwamoto T
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Journal Title
Acta Crystallogr Sect F Struct Biol Cryst Commun 64
Pages: 1125-1127
Peer Reviewed
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[Presentation] Cardiac-specific overexpression of NCX1.1 mutant induces dilated cardiomyopathy in mice2010
Author(s)
Kita S, Iyoda T, Yamamoto S, Adachi-Akahane S, Fukusaki K, Takamatsu H, Sato T, Nakayama H, Du C, Morimoto S, Arai Y, Iwamoto T
Organizer
XX world congress of the ISHR
Place of Presentation
Kyoto
Year and Date
2010-05-14
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[Presentation] Pericyte recruitment of the Ncx1^<-/-> mouse embryo brain inside vasculature2010
Author(s)
山本誠士,村松昌,東英梨月,堂本光子,喜多紗斗美,岩本隆宏,大澤毅,高橋宏行,渡邊泰秀,小室一成,高野健一,新飯田俊平,澁谷正史,松田直之,服部裕一
Organizer
第83回日本薬理学会年会
Place of Presentation
大阪
Year and Date
2010-03-16
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[Presentation] Cardiac-specific overexpression of NCX1.1 mutant induces dilated cardiomyopathy in mice2009
Author(s)
Kita S, Iyoda T, Adachi-Akahane S, Fukusaki K, Takamatsu H, Sato T, Nakaya H, Du CK, Morimoto S, Arai Y, Iwamoto T
Organizer
XXXVI International Congress of Phisiological Science
Place of Presentation
Kyoto
Year and Date
2009-07-27
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