2010 Fiscal Year Final Research Report
Molecular mechanism of abnormal pH-homeostasis in the lumen of acidic organele from DjA2 knock out mice
| Project/Area Number |
20590287
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
TERADA Kazutoyo Kumamoto University, 大学院・生命科学研究部, 准教授 (00253724)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 分子シャペロン / HSP40 / ノックアウトマウス / 酸性 / pH |
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| Research Abstract |
The DjA2 knockout mice showed lethal respiratory failure after birth. This was caused by insufficient alveolar expansion and the type 2 pneumocytes from the knockout mice had abnormal acidity in the lumen of lysosomal acidic organelle. To elucidate the molecular mechanism of maintaining homeostasis in the acidic organelle, I analyzed the pnumocytes by a physiological approach using ratiometry, the DjA2 by a molecular interaction study using Biacore, and the lung tissue by a proteomics approach.
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