2010 Fiscal Year Final Research Report
Identification of novel acceptor proteins for polyADP-ribosylation, determination of the site of modification and clarification of biological function
| Project/Area Number |
20590291
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Nagahama Institute of Bio-Science and Technology |
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Principal Investigator |
MIWA Masanao Nagahama Institute of Bio-Science and Technology, バイオサイエンス学部, 教授 (20012750)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 翻訳後修飾 / ポリADP-リボシル化 / アフィニティークロマトグラフィー / モノクローン抗体 / 神経細胞死 |
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| Research Abstract |
PolyADP-ribosylation is a posttranslational modification that is involved in various cellular events including cell growth and cell death. We are trying to clarify the function of polyADP-ribosylation using Drosophila as an animal model for future application to analyze the mechanism of neurological diseases. We found 9-14 new acceptor proteins of polyADP-ribosylation using 2-D gel electrophoresis and now trying to identify those new proteins by mass spectroscopy.
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[Journal Article] Approaches to separate and identify polyADP-ribosylated proteins using PARG knockout Drosophila.2011
Author(s)
Nodono, H., Hamada, A., Kuroda, Y., Hasegawa, M., Kondo, C., Che, F.S., Hanai, S., Miwa, M.
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Journal Title
Methods in Molecular Biology on Poly(ADP-ribose)(A.Tulin ed., Humana Press Inc., Totowa, NJ, USA) in press
Peer Reviewed
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