Analysis of signal transduction in a novel mouse model.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590299
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: WAKAO Hiroshi Hokkaido University, 大学院・医学研究科, 助教 (10280950)
• Project Period (FY): 2008 – 2010
• Keywords: 胚性幹(ES)細胞 / 免疫制御性T細胞 / TCR

Research Abstract

We have tried to differentiate immune-regulatory cells that play an important role in maintaining the immune-balance in the body from the embryonic stem (ES) cell. From our previous study, it is known that the rearranged configuration of the TCR plays an important role in a selective differentiation of the immune-regulatory cells. We have introduced such a TCR by the lentiviral vector into ES cells and let them differentiate into T cells. However, few immune-regulatory cells developed under such conditions. Our data indicate that the conversion of immune-regulatory cells into iPS cells or the nuclear transfer of such cells will be required to induce immune-regulatory cells.

Research Products

• [Journal Article] Constitutively active Stat5A and Stat5B promote adipogenesis. (2010)
  • Author(s): Wakao H., Wakao, R, Oda, A., Fujita, H.
  • Journal Title: Environmental Health and Preventive Medicine In press
  • Peer Reviewed
• [Journal Article] Regulation of ghrelin signaling by a leptin-induced gene, negative regulatory element-binding protein, in the hypothalamic neurons. (2010)
  • Author(s): Komori T, Doi A, Furuta H, Wakao H., Nakao N, Nakazato M, Nanjo K, Senba E, Morikawa Y.
  • Journal Title: J Biol Chem. 285(48) Pages: 37884-9
  • Peer Reviewed
• [Journal Article] Differentiation of iNKT cells from embryonic stem cells. (2010)
  • Author(s): 若尾宏
  • Journal Title: Curr.Immunol.Rev. 6(2) Pages: 102-108
  • Peer Reviewed
• [Journal Article] NKT cells: from totipotency to regenerative medicine. (2009)
  • Author(s): 若尾宏
  • Journal Title: Arch Immunol Ther Exp (Warsz). 57(2) Pages: 117-28
  • Peer Reviewed
• [Journal Article] 末梢リンパ球の全能性とその応用生化学 (2009)
  • Author(s): 若尾宏
  • Journal Title: 日本生化学会 81 Pages: 277-289
• [Journal Article] In vitro induction of natural killer T cells from embryonic stem cells prepared using somatic cell nuclear transfer. (2008)
  • Author(s): 若尾宏, Wakao R, Sakata S, Iwabuchi K, Oda A, Fujita H.
  • Journal Title: FASEB J. 22(7) Pages: 2223-31
  • Peer Reviewed
• [Remarks] ホームページ等