2010 Fiscal Year Final Research Report

3D nuclear architecture of the regions close to the candidate gene (s) related to the constitutional chromosomal abnormalities

Research Project

Project/Area Number	20590328
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Human genetics
Research Institution	Shinshu University
Principal Investigator	WAKUI Keiko Shinshu University, 医学部, 助教 (50324249)
Co-Investigator(Kenkyū-buntansha)	FUKUSHIMA Yoshimitsu 信州大学, 医学部, 教授 (70273084) TANABE Hideyuki 総合研究大学院大学, 先導科学研究科, 准教授 (50261178)
Research Collaborator	KAWAMURA Rie 信州大学, 大学院・医学研究科, 大学院生
Project Period (FY)	2008 – 2010
Keywords	細胞遺伝学 / 3D-FISH解析 / 染色体構造異常 / 遺伝子 / ゲノム / 位置効果
Research Abstract	3D-FISH analyses performed to measure the relative positioning of SNRPN and UBE3A genes at 15q11.2 region on each homologous chromosomes 15 within the interphase nucleus of normal B-lymphoblastoid cell lines. The average distances between two genes on each chromosome 15 were 0.32 and 0.60μm. This result suggests the 3D distance between two genes on one of the chromosome 15 seems to be different from on the other chromosome 15 in each nucleus. The minimal distances from the SNRPN genes to the nuclear membrane were 0.6±0.7 and 1.4±0.9μm, and also seems to be different between the homologous chromosomes.

(3 results)