Investigation of disease-specific MMP to conquer acute coronary syndrome ?study with transgenic rabbit

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590343
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Saga University
• Principal Investigator: WATANABE Teruo Saga University, 総合分析実験センター, 客員研究員 (40037396)
• Co-Investigator(Kenkyū-buntansha): NISHIJIMA Kazutoshi 佐賀大学, 総合分析実験センター, 助教 (70435874)
• Project Period (FY): 2008 – 2010
• Keywords: MMP / 動脈硬化 / 遺伝子組換え / ウサギ / 病体モデル

Research Abstract

We established MMP-1 and MMP-9 transgenic rabbits, which express the enzymes in their macrophages. MMP-9 Tg rabbits have granuloma-like lesionin their toes. Female MMP-9 and MMP-12 Tg rabbits were mated with WHHL MI, and hybrid offsprings were obtained.

Research Products

• [Journal Article] Human C-reactive protein does not promote atherosclerosis in transgenic rabbits. (2009)
  • Author(s): Koike T, Kitajima S, Yu Y, Nishijima K, Zhang J, Ozaki Y, Morimoto M, Watanabe T, Bhakdi S, Asada Y, Chen YE, Fan J.
  • Journal Title: Circulation 120 Pages: 2088-2094
  • Peer Reviewed
• [Journal Article] Expression of human apoAII in transgenic rabbits leads to dyslipidemia : a new model for combined hyperlipidemia. (2009)
  • Author(s): Koike T, Kitajima S, Yu Y, Li Y, Nishijima K, Liu E, Sun H, Waqar AB, Shibata N, Inoue T, Wang Y, Zhang B, Kobayashi J, Morimoto M, Saku K, Watanabe T, Fan J.
  • Journal Title: Arterioscler Thromb Vasc Biol 29 Pages: 2047-2053
  • Peer Reviewed
• [Journal Article] Temporal and quantitative analysis of expression of metalloproteinases (MMPs) and their endogenous inhibitors in atherosclerotic lesions. (2008)
  • Author(s): Yu Y, Koike T, Kitajima S, Liu E, Morimoto M, Shiomi M, Hatakeyama K, Asada Y, Wang K, Sasaguri Y, Watanabe T, Fan J.
  • Journal Title: Histol Histopathol 23 Pages: 1503-1516
  • Peer Reviewed
• [Journal Article] Matrix metalloproteinase 12 accelerates theinitiation of atherosclerosis and stimulates the progression of fatty streaks to fibrous plaques in transgenic rabbits. (2008)
  • Author(s): Yamada S, Wang K-Y, Tanimoto A, Fan J, Shimajiri S, Kitajima S, Morimoto M, Tsutsui M, Watanabe T, Yasumoto K, Sasaguri Y.
  • Journal Title: Am J Pathol 172 Pages: 1419-1429
  • Peer Reviewed
• [Presentation] Expression of Human ApoAII in Transgenic Rabbits Leads to Dyslipidemia -A New Model for Combined Hyperlipidemia. (2010)
  • Author(s): Koike T, Kitajima S, Yu Y, Nishijima K, Waqar AB, Inoue T, Wang Y, Bo Z, Kobayashi J, Morimoto M, Saku K, Watanabe T, Fan J
  • Organizer: 第42回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 岐阜市
  • Year and Date: 20100715-20100716
• [Presentation] 動脈硬化の発生におけるC反応性蛋白の役割:遺伝子改変ウサギ・モデルによる解析 (2010)
  • Author(s): 範江林, 北嶋修司, 浅田祐士郎, 渡辺照男
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京都
  • Year and Date: 20100427-20100429
• [Presentation] 北嶋修司長期保存された遺伝子改変ウサギ凍結精子の受精能の検討 (2010)
  • Author(s): 西島和俊, 山口慎二, 森本正敏, 渡辺照男
  • Organizer: 第4回ウサギフォーラム
  • Place of Presentation: 秋田市
  • Year and Date: 2010-07-24
• [Presentation] Transgenic rabbits expressing Human C-reactive protein. (2009)
  • Author(s): Koike T, Kitajima S, Yu Y, Nishijima K, Zhang J, Ozaki Y, Morimoto M, Watanabe T, Asada Y, Chen E, Fan J
  • Organizer: 第41回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 下関市
  • Year and Date: 20090717-20090718
• [Presentation] ウサギ採卵成績に影響を及ぼす要因の検討:過去5年間の採卵成績の解析 (2009)
  • Author(s): 北嶋修司, 西島和俊, 森本正敏, 渡辺照男, 範江林
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: さいたま市
  • Year and Date: 20090514-20090516
• [Presentation] 細胞外基質分解酵素MMP-12は、初期動脈硬化巣の形成から進展に重要な促進因子である (2008)
  • Author(s): 山田壮亮, 王克〓, 谷本昭英, 範江林, 島尻正平, 北嶋修司, 森本正敏, 渡邉照男, 笹栗靖之
  • Organizer: 第三回ウサギフォーラム
  • Place of Presentation: 神戸市
  • Year and Date: 2008-07-26
• [Remarks] ホームページ等
  • URL: http://www.animal.med.saga-u.ac.jp/