2010 Fiscal Year Final Research Report
Association between expression of folate-metabolizing and 5-FU target enzymes and effects of chemotherapy in colorectal cancer
| Project/Area Number |
20590355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kobe University |
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Principal Investigator |
KAMOSHIDA Shingo Kobe University, 保健学研究科, 教授 (70351020)
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| Co-Investigator(Kenkyū-buntansha) |
SHINTANI Michiko (田中 路子) 神戸大学, 保健学研究科, 助教 (40207147)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUTSUMI Yutaka 藤田保健衛生大学, 医学部, 教授 (80138643)
MATSUOKA Hiroshi 藤田保健衛生大学, 医学部, 講師 (40367719)
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| Research Collaborator |
SAKAMOTO Kazuki 大鵬薬品工業株式会社, 徳島研究センター, 研究員
SANGAWA Akiko 大阪赤十字病院, 病理部, 臨床検査技師
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| Project Period (FY) |
2008 – 2010
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| Keywords | 胃癌 / 大腸癌 / 葉酸代謝酵素 / ピリミジン合成酵素 / 細胞死 / p53遺伝子 / 薬剤効果予測 / 免疫組織化学 |
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| Research Abstract |
Reproducible detection of folate metabolism-related proteins in routine and pathological samples presented difficulties. However, immunostaining was useful for analyzing expression of pyrimidine-synthesizing enzymes and cell death markers. High expression of thymidine kinase-1 and thymidylate synthase was detected prominently in gastrointestinal and uterine carcinomas. Spontaneous levels of cleaved (active) caspase-9 (CC9) correlated with antitumor effect of S-1, an oral fluoropyrimidine. Gastric carcinomas outnumbered in CC9-positive tumors while colorectal carcinomas in LC3-positive tumors.
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