2010 Fiscal Year Final Research Report
The significance of 53BP1 nuclear foci as a universal tumor tissue marker
| Project/Area Number |
20590367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKASHIMA Masahiro Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (50284683)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 癌 / 53BP1 / ゲノム不安定性 / 蛍光免疫染色 |
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| Research Abstract |
The presence of sporadic DNA double strand breaks (DSBs) is considered as a characteristic feature of genomic instability (GIN). It has been proposed that GIN has a crucial role during several carcinogenesis, and is associated with malignant potential of neoplasms. The presence of 53BP1 nuclear foci (NF) can be a cytologic marker for endogenous DSBs reflecting GIN. This study analyzed the level of GIN, as detected with immunofluorescence of 53BP1 expression, in a variety of resected tumors to evaluate the significance of 53BP1 expression as a universal tumor tissue marker. In results : 1) a number of 53BP1NF and abnormal type of 53BP1 expression were demonstrated in thyroid cancers, suggesting a high level of GIN ; 2) during skin carcinogenesis, GIN seems to be induced at the precancerous stage with constitutive activation of DNA damage response (DDR). Furthermore, invasive cancers exhibited abnormal 53BP1 staining, reflecting a disruption of DDR; 3) The number of 53BP1NF in uterine cervical cells appeared to increase with progression during carcinogenesis and to be significantly associated with the expression of human papilloma virus (HPV) signals.
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