Analysis of basic mechanisms of osteoclastogenesis to apply to clinical study

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590400
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tottori University
• Principal Investigator: HAYASHI Shin-ichi Tottori University, 医学部, 教授 (50208617)
• Project Period (FY): 2008 – 2010
• Keywords: 炎症 / 破骨細胞 / 免疫学 / 骨吸収 / 発生・分化 / 病理学 / 再生医学

Research Abstract

Bones are resorbed both in the steady and pathological states ; however, it is not clear whether the regulation of osteoclastogenesis is same. Here, I detected the presence of the regulation of bone resorption from extramedullary sites. Intramedullary T lineage cells but also the cells in the peritoneal cavity might control the osteoclastogenesis. These results suggest that the novel therapy for bone diseases such as osteoporosis or arthritis will be opened.

Research Products

• [Journal Article] HLA-A2-restricted glypican-3 peptide-specific CTL clones induced by peptide vaccine show high avidity and antigen-specific killing activity against tumor cells. (2011)
  • Author(s): Yoshikawa, T., Nakatsugawa, M., Suzuki, S., Shirakawa, H., Nobuoka, D., Sakemura, N., Motomura, Y., Tanaka-Harada, Y., Hayashi, S.I., Nakatsura, T.
  • Journal Title: Cancer Sci. 102 Pages: 918-925
• [Journal Article] A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells. (2010)
  • Author(s): Murata, A., Okuyama, K., Sakano, S., Kajiki, M., Hirata, T., Yagita, H., Zuniga-Pflucker, J.C., Miyake, K., Akashi-Takamura, S., Moriwaki, S., Niida, S., Yoshino, M., Hayashi, S.I.
  • Journal Title: J.Immunol. 185 Pages: 3905-3912
• [Journal Article] Comparison of osteoclast precursors in peripheral blood mononuclear cells from rheumatoid arthritis and osteoporosis patients. (2009)
  • Author(s): Nose, M., Yamazaki, H., Hagino, H., Morio, Y., Hayashi, S.I., Teshima, R.
  • Journal Title: J.Bone Miner.Metab. 27 Pages: 57-65
• [Journal Article] Suppression of macrophage functions impairs skeletal muscle regeneration with severe fibrosis. (2008)
  • Author(s): Segawa, M., Fukada, S., Yamamoto, Y., Yahagi, H., Kanematsu, M., Sato, M., Ito, T., Uezumi, A., Hayashi, S.I., Miyagoe-Suzuki, Y., Takeda, S., Tsujikawa, K., Yamamoto, H.
  • Journal Title: Exp.Cell Res. 314 Pages: 3232-3244
• [Journal Article] Isolation and characterization of Kit-independent melanocyte precursors induced in the skin of Steel factor transgenic mice. (2008)
  • Author(s): Kawaguchi, A., Chiba, K., Tanimura, Y., Motohashi, T., Aoki, H., Takeda, T., Hayashi, S.I., Shimizu, K., Kunisada, T.
  • Journal Title: Develop. Growth Differ. 50 Pages: 63-69
• [Journal Article] Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. (2008)
  • Author(s): Yoshino, M., Yamazaki, H., Hayashi, S.I.
  • Journal Title: Immunol. Lett. 115 Pages: 131-137
• [Presentation] Distinct mechanisms of self-antigen transport from the epidermis and the dermis to regional lymph nodes. (2010)
  • Author(s): Yoshino, M., Okuyama, K., Murata, A., Egawa, Y., Sawada, A., Hayashi, S.I.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 20100000
• [Presentation] Detection of bone-marrow precursors given rise to B-lineage cells in the presence of delta-like 1. (2010)
  • Author(s): Okuyama, K., Murata, A., Egawa, Y., Sawada, A., Yoshino, M., Hayashi, S.I.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 20100000
• [Presentation] Function of Delta-like 1 as an adhesion molecule. (2010)
  • Author(s): Murata, A., Okuyama, K., Sawada, A., Egawa, Y., Sakano, S., Yagita, H., Zuniga-Pflucker, J.C., Yoshino, M., Hayashi, S.I.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 20100000
• [Presentation] CCR7-independent trafficking of skin antigens to regional lymph nodes by cells in the dermis. (2010)
  • Author(s): Yoshino, M., Okuyama, K., Murata, A., Egawa, Y., Sawada, A., Hayashi, S.I.
  • Organizer: 18^<th> International Symposium on Molecular Cell Biology of Macrophages 2010 : Macrophage Activation and Disease Process.
  • Place of Presentation: 熊本
  • Year and Date: 20100000
• [Presentation] A Notch ligand, Delta-like-1 functions as an adhesion molecule for mast cells. Immune Regulation : Present and Future. (2009)
  • Author(s): Murata, A., Okuyama, K., Sakano, S., Yagita, H., Miyake, K., Wagatsuma, K., Moriyama, T., Yoshino, M., Hayashi, S.I.
  • Organizer: The First International Kishimoto Foundation Symposium.
  • Place of Presentation: 大阪
  • Year and Date: 20090000
• [Presentation] A Notch ligand, Delta-like 1 functions as an adhesion molecule. (2009)
  • Author(s): Murata, A., Okuyama, K., Wagatsuma, K., Moriyama, T., Sakano, S., Yagita, H., Miyake, K., Akashi-Takamura, S., Yoshino, M., Hayashi S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 20090000
• [Presentation] CCR7非依存的に皮膚自己抗原を輸送する細胞の同定/Detection of dermal cells transporting skin antigens in CCR7-independent manner under steady state conditions. (2009)
  • Author(s): Yoshino, M., Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Hayashi S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 20090000
• [Presentation] B220^+ Cd19^+ Flt3^+ Il7rα^+ bone-marrow precursors give rise to B-lineage cells in the presence of a Notch ligand, Delta-like 1. (2009)
  • Author(s): Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Yoshino, M., Hayashi, S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 20090000
• [Presentation] Absence of "natural" IgM-secreting plasma cells in Btk^<xid> bone marrow. (2009)
  • Author(s): Wagatsuma, K., Okuyama, K., Murata, A., Moriyama, T., Yoshino, M., Hayashi, S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 20090000
• [Presentation] Effect of CCR7 on steady-state trafficking of skin dendritic cells to regional lymph nodes. (2008)
  • Author(s): Yoshino, M., Osaka, M., Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Hayashi, S.I.
  • Organizer: The 10th International Symposium on Dendritic Cells.
  • Place of Presentation: 神戸
  • Year and Date: 20080000
• [Presentation] CCR7非依存的な皮膚自己抗原のリンパ節への輸送/CCR7-independent trafficking of skin antigens to regional lymph nodes. (2008)
  • Author(s): Yoshino, M., Osaka, M., Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Hayashi, S.I.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 20080000
• [Presentation] Flt3^+ IL-7R・^+ B220^+ BM precursors given rise to B-lineage cells in the presence of a Notch ligand, Delta-like-1. (2008)
  • Author(s): Okuyama, K., Murata, A., Osaka, M., Moriyama, T., Wagatsuma, K., Yoshino, M., Hayashi, S.I.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 20080000
• [Presentation] A novel function of a Notch ligand, Delta-like-1 as adhesion molecule for mast cells. (2008)
  • Author(s): Murata, A., Okuyama, K., Osaka, M., Moriyama, T., Wagatsuma, K., Sakano, S., Yoshino, M., Hayashi, S.I.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 20080000
• [Book] A Humana Press Book(Methods for investigation of osteoclastogenesis using mouse embryonic stem cells. In"Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases". (Methods in Molecular Biology, Vol.690))(Ed.N.I.zur Nieden) (2011)
  • Author(s): Tsuneto, M., Yamane, T., Hayashi, S.I.
  • Total Pages: 239-253
  • Publisher: Springer Science+Business Media
• [Remarks] ホームページ
  • URL: http://www.med.tottori-u.ac.jp/smgkjm/229/2201.html