Molecular Analysis of Life and Death in Pathogenic Fungi Cryptococcus neoformans : Cell Cycle Cntrol and Interaction between Fungi and Bacteria

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590437
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Chiba University
• Principal Investigator: KAWAMOTO Susumu Chiba University, 真菌医学研究センター, 教授 (80125921)
• Project Period (FY): 2008 – 2010
• Keywords: 真菌 / 感染症 / 細胞周期 / 遺伝子 / タンパク質 / 国際情報交換 / チェコ:ハンガリー

Research Abstract

The G1 Cyclin CnCln1 gene, a key molecule in the cell cycle control in a pathogenic yeast, Cryptococcus neoformans, was cloned, identified and characterized. Interaction between cell cycle control and stress (hypoxia) response was analyzed. Also, localization of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus was demonstrated by scanning immunoelectron microscopy.

Research Products

• [Journal Article] The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression but has important roles in the proper commitment to DNA synthesis and bud emergence in this yeast. (2010)
  • Author(s): Virutudazo EV, Kawamoto S, Ohkusu M, Aoki S, Sipiczki M, Takeo K
  • Journal Title: FEMS Yeast Research 10(5) Pages: 605-618
  • Peer Reviewed
• [Journal Article] Localization by scanning immunoelectron microscopy of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus. (2010)
  • Author(s): Yamaguchi M, Ikeda R, Nishimura M, Kawamoto S
  • Journal Title: Microbiol Immunol 54 Pages: 368-370
  • Peer Reviewed
• [Journal Article] Deletion of CnLIG4 DNA ligase gene in the fungal pathogen Cryptococcus neoformans elevates homologous recombination efficiency. (2010)
  • Author(s): Shimizu K, Li H-M, Virtudazo EV, Watanabe A, Kamei K, Yamaguchi M, Kawamoto S
  • Journal Title: Mycoscience 51 Pages: 28-33
  • Peer Reviewed
• [Journal Article] Non-homologous end joining pathway of the human pathogen Cryptococcus neoformans influences homologous integration efficiency but not virulence. (2010)
  • Author(s): Li H-M, Virtudazo EV, Toh-e A, Yamaguchi M, Kawamoto S, Shimizu K
  • Journal Title: Mycoscience 51 Pages: 272-280
  • Peer Reviewed
• [Journal Article] Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats. (2009)
  • Author(s): Raclavsky V, Husickova V, Moranova Z, Ohkusu M, Fischer O, Precek J, Trtkova J, Takeo K, Kawamoto S
  • Journal Title: Folia Microbiol 54(4) Pages: 349-352
  • Peer Reviewed
• [Journal Article] Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates. (2009)
  • Author(s): Raclavsky V, Pavlicek J, Novotny R, Moranova Z, Ohkusu M, Trtkova J, Takeo K, Kawamoto S
  • Journal Title: Folia Microbiol 54(4) Pages: 369-371
  • Peer Reviewed
• [Journal Article] Hypoxia sensing in Cryptococcus neoformans : Biofilm-like adaptation for dormancy? (2009)
  • Author(s): Moranova Z, Kawamoto S, Raclavsky V
  • Journal Title: Biomedical Papers 153(3) Pages: 189-193
  • Peer Reviewed
• [Presentation] アグロバクテリウム法によるCryptococcus neoformans形質転換に関する条件検討 (2010)
  • Author(s): 清水公徳, 太田裕子, 増田恵美, ヴィルトゥダゾ・エリック, 山口正視, 川本進
  • Organizer: 第54回日本医真菌学会総会,真菌誌51(増刊1号):65
  • Place of Presentation: 東京
  • Year and Date: 20101016-20101617
• [Presentation] Cryptococcus neoformans Wee1 kinase is not essential for growth but has a role in G2-M. (2010)
  • Author(s): E.Virtudazo, M.Ohkusu, K.Shimizu, M.Yamaguchi, S.Kawamoto
  • Organizer: 第54回日本医真菌学会総会,真菌誌51(増刊1号):77
  • Place of Presentation: 東京
  • Year and Date: 20101016-20101617
• [Presentation] 真菌細菌の菌種間相互作用における電子顕微鏡的アプローチ (2010)
  • Author(s): 山口正視, 池田玲子, 川本進
  • Organizer: 第54回日本医真菌学会総会,真菌誌51(増刊1号):65
  • Place of Presentation: 東京
  • Year and Date: 20101016-20101017
• [Presentation] Cell-cycle related genes in the pathogenic yeast Cryptococcus neoformans.9th International Mycological Congress (IMC9) (2010)
  • Author(s): Kawamoto S, Virtudazo EV, Ohkusu M, Takeo K
  • Organizer: The Biology of of Fungi
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 20100801-20100806
• [Presentation] The main cell cycle genes in the pathogenic yeast Cryptococcus neoformans. (2010)
  • Author(s): Kawamoto S, Virtudazo EV, Ohkusu M, Sonoda T, Miura S, Takeo K
  • Organizer: 10th European Conference on Fungal Genetics (ECFG10), Leeuwenhorst
  • Place of Presentation: The Netherlands
  • Year and Date: 20100329-20100401
• [Presentation] Two key players of cell cycle regulation in Cryptococcus neoformans, Cyclin-dependent kinase (Cdk) and its regulator (2010)
  • Author(s): 川本進
  • Organizer: Cyclin.
  • Place of Presentation: University of Wisconsin-Madison,Madison, Wisconsin, USA
  • Year and Date: 2010-11-22
• [Presentation] Molecular analysis of cell cycle regulation in Cryptococcus neoformans. (2009)
  • Author(s): Kawamoto S, Virtudazo EV, Ohkusu M, Manova Z, Raclavsky V, Takeo K
  • Organizer: 4th Trends in Medical Mycology.
  • Place of Presentation: Athens,Greece
  • Year and Date: 20091018-20091021
• [Presentation] Functional analysis of genes involved in drug resistance in Cryptococcus neoformans. (2009)
  • Author(s): Shimizu K, Shimizu M, Li H-M, Kawamoto S
  • Organizer: The 17th Congress of the International Society for Human and Animal Mycology (ISHAM 2009).Program and Abstract book p.280.
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20090525-20090529
• [Presentation] Difference in physiology and protein expression pattern of capsule thickness variants of Cryptococcus neoformans. (2009)
  • Author(s): Ohkusu M, Kawamoto S
  • Organizer: ISHAM 2009.Program and Abstract p.355.
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20090525-20090529
• [Presentation] Understanding cell control in the pathogenic yeast Cryptococcus neoformans. (2009)
  • Author(s): Virtudazo, E.V., Ohkusu, M., Ohata, M., Miklos, I., Sipiczki, Matthias, Aoki, S., Takeo, K., Kawamoto S.
  • Organizer: ISHAM 2009.
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 20090525-20090529
• [Presentation] Analysis of the role of the single G1 cyclin, CnCln1, in Cryptococcus neoformans cell cycle. (2009)
  • Author(s): Virtudazo EV., Ohkusu M, Ohata M, Takeo K, Kawamoto S
  • Organizer: ISHAM 2009.
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 20090525-20090529
• [Presentation] Pospisilova K, Raclavsky V : Study of hypoxia response in Cryptococcus neoformans. (2009)
  • Author(s): Moranova., Virtudazo EV, Ohkusu M, Kawamoto S
  • Organizer: ISHAM 2009.
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 20090525-20090529
• [Presentation] Structural and functional analysis of the single G1 cyclin CnCln1 in Cryptococcus neoformans. (2008)
  • Author(s): Virtudazo EV, Ohkusu M, Ohata M, Miklos I, Sipiczki M, TakeoK, Kawamoto S
  • Organizer: 第91回日本細菌学会関東支部総会
  • Place of Presentation: 千葉市
  • Year and Date: 20081023-20081024
• [Presentation] Towards molecular understanding of cell cycle regulation and cell signaling in Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto S, Virtudazo E, Ohata M, Ohkusu M, Shimizu K, Yamaguchi M, Miklos I, Sipiczki M, Moranova Z, Raclavsky V, Takeo K
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki
  • Year and Date: 20080911-20080914
• [Presentation] Cloning and functional analysis of the Cryptococcus neoformans Cdc25/Mih1 phosphatase homologue. (2008)
  • Author(s): Virtudazo EV, Ohata M, Ohkusu M, Miklos I, Sipiczki M, Takeo K, Kawamoto S
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki, Japan.
  • Year and Date: 20080911-20080914
• [Presentation] Analysis of the role of upstream ORFs in the regulation of CnCln1 expression in Cryptococcus neoformans. (2008)
  • Author(s): Ohata M., Virtudazo EV., Ohkusu M., Takeo K., Kawamoto S
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki, Japan.
  • Year and Date: 20080911-20080914
• [Presentation] Study of hypoxia response in Cryptococcus neoformans. (2008)
  • Author(s): Moranova Z, Husiokova V, Ohkusu M, Kawamoto S, Raclavsky V
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki
  • Year and Date: 20080911-20080914
• [Presentation] Cryptococcus neoformansのCdc25ホスファターゼホモログのクローニングと構造機能解析 (2008)
  • Author(s): ヴィルトゥダゾ・エリック, 大畑美穂子, 大楠美佐子, Ida Miklos, Matthias Sipiczki, 竹尾漢治, 川本進
  • Organizer: 第52回日本医真菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 20080910-20080911
• [Presentation] Cryptococcus neoformansの細胞周期制御におけるG1サイクリン遺伝子upstreamORFの役割 (2008)
  • Author(s): 大畑美穂子, ヴィルトゥダゾ・エリック, 大楠美佐子, 竹尾漢治, 川本進
  • Organizer: 第52回日本医真菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 20080910-20080911
• [Presentation] Molecular approach to understanding cell regulation in a pathogenic yeast Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto S, Virtudazo E, Ohata M, Ohkusu M, Takeo K
  • Organizer: China-Japan Pan Asia Pacific Mycology Forum, Changchun 2008 Changchun
  • Place of Presentation: China
  • Year and Date: 20080728-20080805
• [Presentation] Structural and functional analysis of cell cycle control genes in Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto S, Virtudazo E, Ohkusu M, Sonoda T, Miyagi Y, Okuda K, Takeo K
  • Organizer: 2008 Annual Meeting American Society for Biochemistry and Molecular Biology (Experimental Biology 2008)
  • Place of Presentation: San Diego, California, USA
  • Year and Date: 20080405-20080409
• [Presentation] 病原酵母クリプトコックスの細胞周期制御の分子的理解に向けて (2008)
  • Author(s): 川本進, E.Virtudazo, 大楠美佐子, 竹尾漢治, 田村裕, Z.Moranova, V.Raclavsky
  • Organizer: 第2回真菌ワープ研究会
  • Place of Presentation: 千葉
  • Year and Date: 2008-08-21
• [Book] 酵素利用技術大系～基礎・解析から改変・高機能化・産業利用まで(小宮山真監修)(酵素を創る-生物学的アプローチ-細胞を用いたタンパク質合成:昆虫培養細胞・バキュロウイルスベクター発現系を用いたタンパク質合成) (2010)
  • Author(s): 川本進
  • Total Pages: 172-175
  • Publisher: エヌ・テイー・エス