2010 Fiscal Year Final Research Report
Identification of low-molecular-mass secretory proteins of Mycobacterium tuberculosis and the application to development of anti-tuberculosis vaccines
| Project/Area Number |
20590438
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
NAGATA Toshi Hamamatsu University School of Medicine, 医学部, 教授 (90275024)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMURA Kunio 浜松医科大学, 医学部, 准教授 (10227407)
KOIDE Yukio 浜松医科大学, 医学部, 理事 (30126809)
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| Project Period (FY) |
2008 – 2010
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| Keywords | DNAワクチン / 結核 / 低分子量分泌タンパク / T細胞エピトープ |
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| Research Abstract |
We identified here murine and human dominant T-cell epitopes of three low-molecular-mass secretory proteins, CFP11 (Rv2433c), CFP17 (Rv1827), TB18.5 (Rv0164) of Mycobacterium tuberculosis. Plasmid DNAs encoding these proteins were immunized to two inbred mice and HLA-DRB1^*0401 transgenic mice. T-cell epitopes were determined based on IFN-γ production from immune spleen cells. The results obtained here is feasible for analysis of the role of antigen-specific T cells during M.tuberculosis infection and the vaccine development.
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[Journal Article] Identification of HLA-A*0201-restricted T-cell epitope on the MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis, by MPT51 overlapping peptide screening.2008
Author(s)
Aoshi T, Nagata T, Suzuki M, Uchijima M, Hashimoto D, Rafiei A, Suda T, Chida K, Koide Y
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Journal Title
Infect Immun 76
Pages: 1565-1571
Peer Reviewed
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