2010 Fiscal Year Final Research Report
Modification of host immunity by mycobacteria : role of cell wall glycolipids
| Project/Area Number |
20590441
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
MORITA Yasuhiro Osaka University, 微生物病研究所, 助教 (70397769)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 感染免疫 / マイコバクテリア / 細胞壁 / 糖脂質 |
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| Research Abstract |
Cell wall glycolipids of mycobacteria have been suggested to be important for host immune modulation. In this study, we first clarified the importance of controlling the expression levels of two glycosyltransferases involved in the biosynthesis of these glycolipids. We then revealed that the structural alterations of these glycolipids make both Mycobacterium smegmatis and Mycobacterium tuberculosis vulnerable in terms of cell wall permeability. In addition, we demonstrated the presence of phosphatidylinositol 3-phosphate in Mycobacterium smegmatis for the first time.
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[Journal Article] Inositol lipid metabolism in mycobacteria : biosynthesis and regulatory mechanisms.2011
Author(s)
Morita, Y.S., Fukuda, T., Sena, C.B., Yamaryo-Botte, Y., McConville, M.J., Kinoshita, T.
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Journal Title
Biochim. Biophys. Acta, in press
Peer Reviewed
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[Journal Article] Stress-induced synthesis of phosphatidylinositol 3-phosphate in mycobacteria.2010
Author(s)
Morita, Y.S., Yamaryo-Botte, Y., Miyanagi, K., Callaghan, J.M., Patterson, J.H., Crellin, P.K., Coppel, R.L., Billman-Jacobe, H., Kinoshita, T., McConville, M.J.
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Journal Title
J.Biol. Chem. 285
Pages: 16643-50
Peer Reviewed
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[Journal Article] Controlled expression of branch-forming mannosyltransferase is critical for mycobacterial lipoarabinomannan biosynthesis.2010
Author(s)
Sena, C.B., Fukuda, T., Miyanagi, K., Matsumoto, S., Kobayashi, K.,Murakami, Y., Maeda, Y., Kinoshita, T., Morita, Y.S.
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Journal Title
J.Biol. Chem. 285
Pages: 13326-36
Peer Reviewed
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[Journal Article] Direct recognition of the mycobacterial glycolipid, trehalosedimycolate, by C-type lectin Mincle : Mincle isareceptor formycobacterial glycolipid.2009
Author(s)
Ishikawa, E., Ishikawa, T., Morita, Y.S., Toyonaga, K., Yamada, H., Takeuchi, O., Kinoshita, T., Akira, S., Yoshikai, Y., Yamasaki, S.
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Journal Title
J.Exp. Med. 206
Pages: 2879-88
Peer Reviewed
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[Journal Article] Mutations in pimE restore lipoarabinomannan synthesis and growth in a Mycobacterium smegmatislpqW mutant.2008
Author(s)
Crellin, P.K., Kovacevic, S., Martin, K.L., Brammananth, R., Morita, Y.S., Billman-Jacobe, H., McConville, M.J., Coppel, R.L.
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Journal Title
J.Bacteriol. 190
Pages: 3690-3699
Peer Reviewed
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[Presentation] Structural alterations of lipomannan and lipoarabinomannanaffect the integrity of mycobacterialcell wall.2011
Author(s)
Fukuda, T., Miyanagi, K., Hamasaki, M., Yoshimori, T., Murakami, Y., Maeda, Y., Kinoshita, T., Morita, Y.S.
Organizer
Keystone Symposia of Molecular and Cellular Biology : Mycobacteria : Physiology, Metabolism and Pathogenesis. Back to the Basics
Place of Presentation
Vancouver Canada
Year and Date
2011-05-17
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[Presentation] Stress-inducedsynthesis of phosphatidylinositol3-phosphate in mycobacteria.2010
Author(s)
Morita, Y.S., Yamaryo-Botte, Y., Miyanagi, K., Crellin, P., Coppel, R.L., Kinoshita, T., McConville, M.J.
Organizer
OzBio2010 (incorporating the 12th IUBMB conference).
Place of Presentation
Melbourne,Australia
Year and Date
2010-12-30
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[Presentation] The effects of structural alterations oflipomannan and lipoarabinomannan on mycobacterial cell wall.2010
Author(s)
Fukuda, T., Miyanagi, K., Hamasaki, M., Yoshimori, T., Maeda, Y., Kinoshita,T., Morita, Y.S.
Organizer
The 83rdAnnual Meeting of the Japanese Biochemical Society
Place of Presentation
Kobe, Japan
Year and Date
2010-12-09
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[Presentation] Controlledexpression of branch-formingmannosyltransferase is critical forthe biosynthesis of lipoarabinomannan in mycobacteria.2010
Author(s)
Sena, C.B., Fukuda, T., Miyanagi, K., Matsumoto, S., Kobayashi, K., Murakami, Y., Maeda, Y., Kinoshita, T., Morita, Y.S.
Organizer
XXVth International Carbohydrate Symposium
Place of Presentation
Chiba, Japan
Year and Date
2010-08-05
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[Presentation] Compartmentalization of glycolipid biosynthesis in mycobacteria.2008
Author(s)
Morita, Y.S., Nakatani, F., McMillan, B.N.I., Billman-Jacobe, H.,McConville, M.J., Maeda, Y., Kinoshita, T.
Organizer
Human Frontier Science Program 8th Annual Awardees Meeting
Place of Presentation
Berlin, Germany
Year and Date
2008-07-07
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[Presentation] Mannosyltransferase required for α1,2-mannosylation of lipomannan and lipoarabinomannan in Mycobacterium bovis bacillus Calmette-Guerin.2008
Author(s)
Sena, C.B., Matsumoto, S., Kobayashi, K., McConville, M.J., Maeda, Y., Kinoshita, T., Morita, Y.S.
Organizer
The Seventh International Conference on the Pathogenesis of MycobacterialInfections
Place of Presentation
Stockholm, Sweden
Year and Date
2008-06-27
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[Presentation] Subcellular compartmentalization of membrane-bound enzymes involved inthe synthesis of phosphatidylinositolmannosides in Mycobacterium smegmatis.2008
Author(s)
Morita, Y.S., Fukuda, T., Billman-Jacobe, H., McConville, M.J., Maeda, Y., Kinoshita, T.
Organizer
The Seventh International Conference on the Pathogenesis of Mycobacterial Infections
Place of Presentation
Stockholm, Sweden
Year and Date
2008-06-27
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[Book] Achapterin "The Enzymes, Volume 26, Glycosylphosphatidylinositol(GPI) Anchoring of Proteins". (Menon,A.K., Kinoshita, T., Orlean, P., Tamanoi, F., Editors) Academic Press.2009
Author(s)
Maeda, Y., Morita, Y.S.
Total Pages
91-115
Publisher
Mannosylation.
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