2010 Fiscal Year Final Research Report
Antigen presentation and cytokine resposes against mycobial glycolipids.
| Project/Area Number |
20590453
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Iwaki Meisei University |
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Principal Investigator |
KUMAZAWA Yoshio Iwaki Meisei University, 薬学部, 客員研究員 (30072375)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIMOTO Hiroaki 北里大学, 理学部, 講師 (00253534)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 感染免疫 |
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| Research Abstract |
The present study has aimed at the antigen presentation and cytokine responses against mycobacterial glycolipids using the guinea pig. Guinea pigs were inoculated intradermally with M.bovis BCG. Mycobacterial lipids specific serum IgG antibody titer was measured by ELISA. BCG-infected guinea pigs had IgG responses against PPD and phosphatidylinositol mannoside (PIM). Proliferation of T cells specific for mycobacterial lipids was estimated by tritiated-thymidine uptake. In BCG-infected guinea pig, splenic T cells showed proliferative responses to PPD, PIM and trehalose 6,6'-dimicolate (TDM).
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