Molecular biology and epidemiological analysis of Ent plasmid transmission in Enterotoxigenic Escherichia coli.

2012 Fiscal Year Final Research Report

• Project/Area Number: 20590459
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Fujita Health University
• Principal Investigator: OCHI Sadayuki 藤田保健衛生大学, 医学部, 准教授 (80268705)
• Project Period (FY): 2008 – 2012
• Keywords: 病原細菌 / プラスミド

Research Abstract

The complete nucleotide sequence was determined for an Ent plasmid (pEntH10407) in enterotoxigenic Escherichia coli H10407. Sequence analysis indicated that pEntH10407 contains three distinct major regions: (i) a pathogenicity islet containing enterotoxin genes, (ii) a region involved in plasmid replication and maintenance, and (iii) a region including tra genes that cause the self-transmissibility. This raises possibility that the H10407 strain might have been born by self-transmissibility of pEntH10407.

Research Products

• [Journal Article] Role of sphingomyelinase in infectious diseases caused by Bacillus cereus (2012)
  • Author(s): Masataka Oda, Manabu Hashimoto, Masaya Takahashi, Yuka Ohmae, Soshi Seike, Ryoko Kato, Aoi Fujita, Hideaki Tsuge, Masahiro Nagahama, Sadayuki Ochi, Teppei Sasahara,Shunji Hayashi, Yoshikazu Hirai and Jun Sakurai
  • Journal Title: PLoS One Volume: 7(6) Pages: e38054
  • Peer Reviewed
• [Journal Article] Role of phospholipid metabolism and G protein in the action induced by Clostridium perfringens alpha-toxin (2012)
  • Author(s): Masahiro Nagahama, Masataka Oda, Sadayuki Ochi, Keiko Kobayashi and Jun Sakurai
  • Journal Title: J. Glycom. Lipidom Volume: 2 (3) Pages: S3-001
  • DOI: DOI:10.4172/2153-0637.S3-001
  • Peer Reviewed
• [Journal Article] P19 embryonal carcinoma cells exhibit high sensitivity to botulinum type C and D/C mosaic neurotoxins (2012)
  • Author(s): Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Keiji Nakamura, Yoshikazu Tanaka, Nipawan Nuemket, Koki Taniguchi, Shunji Kozaki and Takao Tsuji
  • Journal Title: Microbiol. Immunol Volume: 56(10)
  • DOI: DOI:10.1111/j.1348-0421.2012.00490.x
  • Peer Reviewed
• [Journal Article] Recent insights into Clostridium perfringens alpha-toxin (2011)
  • Author(s): Masahiro Nagahama, Masataka Oda, Sadayuki Ochi, Keiko Kobayashi and Jun Sakurai
  • Journal Title: Res. Adv. Infect. Immun Volume: 1 Pages: 1-10
  • URL: http://www.trnres.com/index.htm
  • Peer Reviewed
• [Journal Article] Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid (2009)
  • Author(s): Sadayuki Ochi, Tohru Shimizu, Kaori Ohtani, Yoshio Ichinose, Hideyuki Arimitsu,Kentaro Tsukamoto, MichioKato and Takao Tsuji
  • Journal Title: DNA Res Volume: 16(5) Pages: 299-309
  • DOI: DOI:10.1093/dnares/dsp015
  • Peer Reviewed
• [Journal Article] Lincomycin-induced over- expression of mature recombinant cholera toxin B subunit and the holotoxin in Escherichia coli (2009)
  • Author(s): Hideyuki Arimitsu, Kentaro Tsukamoto, Sadayuki Ochi, Keiko Sasaki, Michio Kato, Koki Taniguchi, Keiji Oguma and Takao Tsuji
  • Journal Title: Protein Expr. Purif Volume: 67(2) Pages: 96-103
  • DOI: DOI:10.1016/j.pep.2009.04.011
  • Peer Reviewed
• [Journal Article] The relationship between the metabolism of sphingomyelin species and the hemolysis of sheep erythrocytes induced by Clostridium perfringens a-toxin (2008)
  • Author(s): Masataka Oda, Takayuki Matsuno, Ryouta Shiihara, Sadayuki Ochi, Rieko Yamauchi, Yuki Saito, Hiroshi Imagawa, Masahiro Nagahama, Mugio Nishizawa and Jun Sakurai
  • Journal Title: J. Lipid Res Volume: 49(5) Pages: 1039-1047
  • DOI: DOI:10.1194/jlr.M700587-JLR200
  • Peer Reviewed
• [Journal Article] Molecular properties of each subcomponent in Clostridium botulinum type B haemagglutinin complex (2008)
  • Author(s): Hideyuki Arimitsu, Yoshihiko Sakaguchi, Jae-Chul Lee, Sadayuki Ochi, Kentaro Tsukamoto, Yumiko Yamamoto, Shaobo Ma, Takao Tsuji and Keiji Oguma
  • Journal Title: Microb. Pathog Volume: 45 (2) Pages: 142-149
  • DOI: DOI:10.1016/j.micpath.2008.04.007
  • Peer Reviewed
• [Journal Article] Paola Neri and Hiroshi Mori. A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection against Shiga toxin types 1 and 2 (2008)
  • Author(s): Takao Tsuji, Takeshi Shimizu, Keiko Sasaki, Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Toshiyasu Shimizu, Koki Taniguchi, Masatoshi Noda
  • Journal Title: Vaccine Volume: 26(17) Pages: 2092-2099
  • DOI: DOI:10.1016/j.vaccine.2008.02.034
  • Peer Reviewed
• [Journal Article] Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin (2008)
  • Author(s): Takao Tsuji, Takeshi Shimizu, Keiko Sasaki, Yoshiyasu Shimizu, Kentaro Tsukamoto, Hideyuki Arimitsu, Sadayuki Ochi, Satoshi Sugiyama, Koki Taniguchi Paola Neri, Hiroshi Mori
  • Journal Title: Vaccine Volume: 26(4) Pages: 469-476
  • URL: http://www.sciencedirect.com/science/a rticle/pii/S0264410X07013370
  • Peer Reviewed
• [Presentation] Carbohydrate structure of lincomycin activates lac promoter in Escherichia coli (2012)
  • Author(s): Hideyuki Arimitsu, Kentaro Tsukamoto,Sadayuki Ochi, Keiko Sasaki, Takao Tsuji、
  • Organizer: The 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Panel: United States-Japan Cooperative Medical Science Program
  • Place of Presentation: 千葉市
  • Year and Date: 2012-12-13
• [Presentation] ケニアのマンデラ東県で発生した下痢症アウトブレイクの 原因菌の疫学調査 (2012)
  • Author(s): 越智定幸、Martin Bundi、Sora Suka、有満秀幸、塚本健太郎、佐々木慶子、清水 利康、一瀬休生、辻孝雄
  • Organizer: 第48回日本細菌学会中部支部総会
  • Place of Presentation: 金沢市
  • Year and Date: 2012-11-09
• [Presentation] ケニア・マンデラ地方下痢症のアウトブレイクに関連するEAST1遺伝子保有大腸菌 (2012)
  • Author(s): 越智定幸、マーチンブンディ、ソラスカ、嶺直幸、有満秀幸、塚本健太郎、佐々木慶子、清水利康、一瀬休生、辻孝雄
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-03-28
• [Presentation] Enteroaggregative Escherichia coli associated with a massive outbreak of gastrointestinal illness in Mandera, Kenya (2012)
  • Author(s): Martin Bundi, Sadayuki Ochi, Gabriel Miringu, Angela Makumi, Sora Huka, Ernest Wander, Amina Galat, Victor Ager, Mwajuma Abubakar, David Mutonga, D. Langat, Hillary Limo, Z. Irura, Mohamed Karama, Takao Tsuji and Yoshio Ichinose
  • Organizer: 2nd KEMRI Annual Scientific & Health (KASH) Conference
  • Place of Presentation: Nairobi、Kenya
  • Year and Date: 2012-02-08
• [Presentation] Lincomycin-induced overexpression method of mutant Shiga toxin-2 for vaccine antigen (2011)
  • Author(s): Hideyuki Arimitsu, Sadayuki Ochi, Kentaro Tsukamoto, Keiko Sasaki and Takao Tsuji
  • Organizer: The 46th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Panel: United States-Japan Cooperative Medical Science Program
  • Place of Presentation: Kolkata、India
  • Year and Date: 2011-12-14
• [Presentation] Over-expression methods of shiga toxin 1, shiga toxin 2 and their mutants in Escherichia coli for evaluating toxoid vaccine (2011)
  • Author(s): Hideyuki Arimitsu, Kentaro Tsukamoto, Sadayuki Ochi, Keiko Sasaki, Michio Kato and Takao Tsuji
  • Organizer: 第84回日本細菌学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-07
• [Presentation] Structure and activity of receptor binding domain of Clostridium botulinum D/C mosaic neurotoxin (2011)
  • Author(s): Kentaro Tsukamto, Nipawan Nuemket, Yoshikazu Tanaka, Hideyuki Arimitsu, Sadayuki Ochi, Keiji Nakamura, Shunji Kozaki, Isao Tanaka and Takao Tsuji
  • Organizer: 第84回日本細菌学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-07
• [Presentation] Analysis of the transfer and plasmid maintenance regions of the enterotoxigenic Escherichia coli Ent plasmid pEntH10407 (2010)
  • Author(s): Sadayuki Ochi, Tohru Shimizu, Kaori Ohtani, Yoshio Ichinose, Hideyuki Arimitsu, Kentaro Tsukamoto, Michio Kato, Toshiyasu Shimizu and Takao Tsuji
  • Organizer: The 45th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Panel: United States-Japan Cooperative Medical Science Program
  • Place of Presentation: 京都市
  • Year and Date: 2010-12-07
• [Presentation] 毒素原性大腸菌H10407株Entプラスミドの機能領域の配列解析 (2010)
  • Author(s): 越智定幸、清水徹、大谷郁、有満秀幸、塚本健太郎、佐々木慶子、加藤道夫、一瀬休生、辻孝雄
  • Organizer: 第57回トキシンシンポジウム
  • Place of Presentation: 長浜市
  • Year and Date: 2010-07-14
• [Presentation] 毒素原性大腸菌H10407株Entプラスミドの接合伝達領域 (2009)
  • Author(s): 越智定幸、有満秀幸、塚本健太郎、大谷郁、Neri Paola、佐々木慶子、加藤道夫、一瀬休生、清水徹、辻孝雄
  • Organizer: 第46回日本細菌学会中部支部総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-12-23
• [Presentation] Construction of lincomycin-induced expression system of cholera toxin B subunit in Escherichia coli (2008)
  • Author(s): Hideyuki Arimitsu, Kentaro Tsukamoto, Sadayuki Ochi, Keiko Sasaki, and Takao Tsuji
  • Organizer: The 43th Joint Meeting of the US-Japan Cholera and other Bacterial Enteric Infections Panel
  • Place of Presentation: 福岡市
  • Year and Date: 2008-11-18
• [Presentation] 毒素原性大腸菌H10407株Entプラスミドの塩基配列解析 (2008)
  • Author(s): 越智定幸、有満秀幸、塚本健太郎、大谷郁、佐々木慶子、加藤道夫、清水徹、辻孝雄
  • Organizer: 第45回日本細菌学会中部支部総会
  • Place of Presentation: 金沢市
  • Year and Date: 2008-10-17
• [Presentation] 毒素原性大腸菌H10407株Entプラスミドの全塩基配列決定と解析 (2008)
  • Author(s): 越智定幸、有満秀幸、塚本健太郎、大谷郁、佐々木慶子、清水徹、辻孝雄
  • Organizer: 第20回微生物シンポジウム
  • Place of Presentation: 岐阜市
  • Year and Date: 2008-09-03
• [Presentation] (2008)
  • Author(s): 越智定幸、有満秀幸、塚本健太郎、大谷郁、佐々木慶子、加藤道夫、清水徹、辻孝雄
  • Organizer: 第55回毒素シンポジウム
  • Place of Presentation: 南都留郡
  • Year and Date: 2008-07-03