Study on the host factors which regulate hepatitis C virus genome replication

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Okayama University
• Principal Investigator: ARIUMI Yusuo Okayama University, 大学院・医歯薬学総合研究科, 助教 (60303913)
• Research Collaborator: KUROKI Misao 岡山大学, 大学院・医歯薬学総合研究科, 大学院
• Project Period (FY): 2008 – 2010
• Keywords: C型肝炎ウイルス / RNAウイルス / 宿主因子 / DDX3 / ATM / ESCRT / P-body

Research Abstract

We have identified following host factors involved in HCV replication : (1) DNA damage sensor ATM is required for HCV RNA replication. (2) HCV infection disrupts P-body and DDX6 is required for HCV replication. (3) ESCRT system is required for HCV production.

Research Products

• [Journal Article] The ESCRT system is required for hepatitis C virus production (2011)
  • Author(s): Ariumi Y, Kuroki M, Maki M, Ikeda M, Dansako H, Wakita T, Kato N
  • Journal Title: PLoS One 6 Pages: e14517
  • Peer Reviewed
• [Journal Article] Gene expression profile of Li23, a new human hepatoma cell line that enables robust hepatitis C virus replication : Comparison with HuH-7 and other hepatitc cell lines (2010)
  • Author(s): Mori K, Ikeda M, Ariumi Y, Kato N
  • Journal Title: Hepatol Res 401 Pages: 1248-1253
  • Peer Reviewed
• [Journal Article] Amino acid substitutions of hepatitis C virus core protein are not associated with intracellular antiviral response to interferon-α in vitro (2010)
  • Author(s): Ikeda F, Dansako H, Nishimura G, Mori K, Kawai Y, Ariumi Y, Miyake Y, Takaki A, Nouso K, Iwasaki Y, Ikeda M, Kato N, Yamamoto K
  • Journal Title: Liver Int 30 Pages: 1324-1331
  • Peer Reviewed
• [Journal Article] DNA損傷センサーATMキナーゼとChk2はC型肝炎ウイルスのRNA複製に必要である (2010)
  • Author(s): 有海康雄, 黒木美沙緒, 團迫浩方, 阿部健一, 池田正徳, 脇田隆字, 加藤宣之
  • Journal Title: 岡山医学会雑誌 122 Pages: 9-16
  • Peer Reviewed
• [Journal Article] HCV genotype 1b chimeric replicon with NS5B of JFH-1 exhibited resistance to cyclosporine A (2009)
  • Author(s): Abe K, Ikeda M, Ariumi Y, Dansako H, Wakita T, Kato N
  • Journal Title: Arch Virol 154 Pages: 1671-1677
  • Peer Reviewed
• [Journal Article] Efficient replication systems for hepatitis C virus using a new hepatoma cell line (2009)
  • Author(s): Kato N, Mori K, Abe K, Dansako H, Kuroki M, Ariumi Y, Wakita T, Ikeda M
  • Journal Title: Virus Res 146 Pages: 41-50
  • Peer Reviewed
• [Journal Article] Oxidative stress induces anti-hepatitis C virus status via the activation of extracellular signal-regulated kinase (2009)
  • Author(s): Yano M, Ikeda M, Abe K, Kawai Y, Kuroki M, Mori K, Dansako H, Ariumi Y, Ohkoshi S, Aoyagi Y, Kato N
  • Journal Title: Hepatology 50 Pages: 678-688
  • Peer Reviewed
• [Journal Article] Development of a hepatitis C virus relapse model using genome-length hepatitis C virus ribonucleic acid-harboring cells possessing the interferon-alpha-resistance phenotype (2009)
  • Author(s): Kawai Y, Ikeda M, Abe K, Yano M, Ariumi Y, Dansako H, Yamamoto K, Kato N
  • Journal Title: Hepatol Res 39 Pages: 898-909
  • Peer Reviewed
• [Journal Article] Replicons from genotype 1b HCV-positive sera exhibit diverse sensitivities to anti-HCV reagents (2009)
  • Author(s): Nishimura G, Ikeda M, Mori K, Nakazawa T, Ariumi Y, Dansako T, Kato N
  • Journal Title: Antiviral Res 82 Pages: 42-50
  • Peer Reviewed
• [Journal Article] Double-stranded RNA-induced interferon-beta and inflammatory cytokine production modulated by hepatitis C virus serine proteases derived from patients with hepatic diseases (2009)
  • Author(s): Dansako H, Ikeda M, Ariumi Y, Wakita T, Kato N
  • Journal Title: Arch Virol 154 Pages: 801-810
  • Peer Reviewed
• [Journal Article] synergistically inhibits HCV RNA replication in combination with interferon-alpha (2009)
  • Author(s): Ikeda M, Mori K, Ariumi Y, Dansako H, Kato N, Oncostatin M
  • Journal Title: FEBS Lett 583 Pages: 1434-1438
  • Peer Reviewed
• [Journal Article] Arsenic trioxide inhibits hepatitis C virus RNA replication through modulation of the glutathione redox system and oxidative stress (2009)
  • Author(s): Kuroki M, Ariumi Y, Ikeda M, Dansako H, Wakita T, Kato N
  • Journal Title: J Virol 83 Pages: 2338-2348
  • Peer Reviewed
• [Journal Article] Genetic variability and diversity of intracellular genome-length hepatitis C virus RNA in long-term cell culture (2009)
  • Author(s): Kato N, Abe K, Mori K, Ariumi Y, Dansako H, Ikeda M
  • Journal Title: Arch Virol 154 Pages: 77-85
  • Peer Reviewed
• [Journal Article] 肝癌HCVによる発癌メカニズム慢性肝炎から肝癌に至るパスウェイ (2009)
  • Author(s): 有海康雄、加藤宣之
  • Journal Title: 日本臨床 67巻、増刊3号 Pages: 138-142
• [Journal Article] ウイルス感染と発癌 (2009)
  • Author(s): 有海康雄、加藤宣之
  • Journal Title: 臨床検査 53巻 Pages: 29-35
• [Journal Article] The DNA damage sensors ataxia-telangiectasia mutated kinase and checkpoint kinase 2 are required for hepatitis C virus RNA replication (2008)
  • Author(s): Ariumi Y, Kuroki M, Dansako H, Abe K, Ikeda M, Wakita T, Kato N
  • Journal Title: J Virol 82 Pages: 9639-9646
  • Peer Reviewed
• [Journal Article] A new living cell-based assay system for monitoring genome-length hepatitis C virus RNA replication (2008)
  • Author(s): Dansako H, Ikeda M, Abe K, Mori K, Takemoto K, Ariumi Y, Kato N
  • Journal Title: Virus Res 172 Pages: 72-79
  • Peer Reviewed
• [Journal Article] New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C (2008)
  • Author(s): Mori K, Abe K, Dansako H, Ariumi Y, Ikeda M, Kato N
  • Journal Title: Biochem Biophys Res Commun 271 Pages: 104-109
  • Peer Reviewed
• [Journal Article] Mechanism of action of ribavirin in a novel hepatitis C virus replication cell system
  • Author(s): Mori K, Ikeda M, Ariumi Y, Dansako H, Wakita T, Kato N
  • Journal Title: Virus Res in press
  • Peer Reviewed
• [Journal Article] Anti-ulcer agent Teprenone inhibits hepatitis C virus replication : Potential treatment for hepatitis C
  • Author(s): Ikeda M, Kawai Y, Mori K, Yano M, Abe K, Nishimura G, Dansako H, Ariumi Y, Wakita T, Yamamoto K, Kato N
  • Journal Title: Liver Int in press
  • Peer Reviewed
• [Presentation] 癌抑制因子とHCVのクロストーク (2010)
  • Author(s): 有海康雄
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-20101109
• [Presentation] がん抑制因子PMLはHCVのライフサイクルに必須である (2010)
  • Author(s): 黒木美沙緒, 有海康雄, 池田正徳, 團迫浩方, 脇田隆字, 加藤宣之
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 20101107-20101109
• [Presentation] がん抑制因子PMLはHCVの生活環に必須である (2010)
  • Author(s): 黒木美沙緒, 有海康雄, 池田正徳, 團迫浩方, 脇田隆字, 加藤宣之
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 20100922-20100924
• [Presentation] Role of distinct DDX DEAD-box RNA helicases in HCV RNA replication. (2010)
  • Author(s): 有海康雄, 池田正徳, 脇田隆字, 加藤宣之
  • Organizer: 17^<th> International Meeting on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100910-20100914
• [Presentation] ESCRT小胞輸送系のHCV産生への関与 (2009)
  • Author(s): 有海康雄、黒木美沙緒、牧正敏、池田正徳、團迫浩方、脇田隆字、加藤宣之
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20091025-20091027
• [Presentation] 癌抑制因子PMLはHCV粒子産生に必要である (2009)
  • Author(s): 黒木美沙緒、有海康雄、池田正徳、團迫浩方、脇田隆字、加藤宣之
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20091025-20091027
• [Presentation] The ESCRT pathway is required for HCV production. (2009)
  • Author(s): Ariumi Y, Kuroki M, Maki M, Ikeda M, Dansako H, Wakita T, Kato N
  • Organizer: 16^<th> International Meeting on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Nice, France
  • Year and Date: 20091003-20091007
• [Presentation] The PML tumor suppressor protein is required for HCV production. (2009)
  • Author(s): 黒木美沙緒, 有海康雄, 池田正徳, 團迫浩方, 脇田隆字, 加藤宣之
  • Organizer: 16^<th> International Meeting on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: Nice, France
  • Year and Date: 20091003-20091007
• [Presentation] The vacuolar protein sorting pathway is essential for HCV budding. (2008)
  • Author(s): 有海康雄, 黒木美沙緒, 池田正徳, 團迫浩方, 脇田隆字, 加藤宣之
  • Organizer: 15^<th> International Meeting on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: San Antonio, USA
  • Year and Date: 20081005-20081009
• [Presentation] Arsenic trioxide inhibits HCV RNA replication through modulation of oxidative stress. (2008)
  • Author(s): 黒木美沙緒, 有海康雄, 池田正徳, 團迫浩方, 脇田隆字, 加藤宣之
  • Organizer: 15^<th> International Meeting on Hepatitis C Virus and Related Viruses.
  • Place of Presentation: San Antonio, USA
  • Year and Date: 20081005-20081009
• [Remarks] ホームページ等
  • URL: http://www.okayama-u.ac.jp/user/med/dmb/index.html