2010 Fiscal Year Final Research Report
Elucidation of the mechanism underlying core histone-mediated antiviral innate immune response
| Project/Area Number |
20590477
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Yokohama City University |
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Principal Investigator |
TAKESHITA Fumihiko Yokohama City University, 医学研究科, 客員教授 (60333572)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 感染防御 / ウイルス学 / 基礎医学 |
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| Research Abstract |
Previous our research has clarified that H2B could sense viral double stranded DNA (dsDNA), and induce antiviral innate immune response. The goal of current research is to elucidate molecular mechanism of H2B-mediated recognition of cytoplasmic DNA.
The results showed that extranuclear H2B interacts with IPS-1 upon sensing cytoplasmic DNA. We also identified the novel adaptor molecule, named as CIAO, to connect H2B with IPS-1, and showed that H2B-CIAO-IPS-1 complex has an important role to induce type I IFNs upon antiviral innate immune response. Furthermore, chimera protein N'-CARD, that is constructed by fusion of H2B fragment to IPS-1 fragment, could induce strong type I IFNs production, suggesting that H2B-CIAO-IPS-1 complex could be useful target to develop the novel immunomodulating drugs.
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[Journal Article] Immunogenicity of whole-parasite vaccines against Plasmodium falciparum involves malarial hemozoin and host TLR9.2010
Author(s)
Coban, C., Y. Igari, M. Yagi, T. Reimer, S. Koyama, T. Aoshi, K. Ohata, T. Tsukui, F. Takeshita, K. Sakurai, T. Ikegami, A. Nakagawa, T. Horii, G. Nunez, K.J. Ishii, S. Akira
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Journal Title
Cell Host Microbe 7
Pages: 50-61
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[Journal Article] Extrachromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010
Author(s)
Kobiyama, K., F. Takeshita, N. Jounai, A. Sakaue-Sawano, A. Miyawaki, K.J. Ishii, T. Kawai, S. Sasaki, H. Hirano, N. Ishii, K. Okuda, K. Suzuki
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Journal Title
J Virol 84
Pages: 822-832
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[Journal Article] A signaling polypeptide derived from an innate immune adaptor molecule can be harnessed as a new class of vaccine adjuvant.2009
Author(s)
Kobiyama, K., F. Takeshita, K. J. Ishii, S. Koyama, T. Aoshi, S. Akira, A. Sakaue-Sawano, A. Miyawaki, Y. Yamanaka, H. Hirano, K. Suzuki, K. Okuda
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Journal Title
J Immunol 182
Pages: 1593-1601
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