2010 Fiscal Year Final Research Report
Development of the analysis method of proteins in breast cancer for predictdrug sensitivity the prediction of drug sensitivity
| Project/Area Number |
20590532
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Hirosaki University |
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Principal Investigator |
ODAGIRI Hiroki Hirosaki University, 大学院・医学研究科, 准教授 (60250601)
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| Co-Investigator(Kenkyū-buntansha) |
TATEISHI Tomonori 弘前大学, 大学院・医学研究科, 教授 (80236554)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 癌 / 蛋白質 / プロテオーム / 薬物反応性 |
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| Research Abstract |
In the first two years, optimal condition of the two dimensional gel electrophoresis using proteins from breast cancer tissue was decided.
Optimal condition was as follows: applied protein amount for gel was 30μg, pH range was 3-10, voltage was 500v 1h, 1000v 2h, then 8000v 2.5h in the first dimension polyacrylamide gel electrophoresis. Clear dots pattern was obtained in 10mA/gel 1.5h for the second demensional gel electrophoresis. 2D-DIGE (2 dimensional-differential gel electrophoresis) was selected to compare the amount of proteins between the cancer tissue and the non-cancerous tissue quantitatively. Protein from cancer tissue was labeled by Cy3 and protein from non-cancerous tissue was lebeled by Cy5. Difference of the amount of proteins from two kind of tissue was shown by the difference of the intensity of those two kinds of fluorescence.
In 2010, tissue from removed from ten breast cancer patients was tested.
Several dots, which showed difference in the intensity of fluorescence between cancer and non-cancerous tissue, were observed. Those should be candidates of protein that are important when cancer shows malignant characters.
In near future, we will identify the proteins from those dots which differ between cancer and non-cancerous tissue for the better understanding of the carcinogenesis.
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