Clarify the molecular mechanisms of the induction of apoptosis by the highly active nucleosides

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590533
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied pharmacology
• Research Institution: Tohoku University
• Principal Investigator: SATO Takeya Tohoku University, 大学院・医学系研究科, 助教 (10312696)
• Co-Investigator(Kenkyū-buntansha): YANAGISAWA Teruyuki 東北大学, 大学院・医学系研究科, 教授 (90133941) ; SUKEGAWA Jun 東北大学, 大学院・医学系研究科, 准教授 (30187687)
• Project Period (FY): 2008 – 2010
• Keywords: 遺伝子 / 癌 / 生体機能利用 / トランスレーショナルリサーチ / 薬剤反応性

Research Abstract

Bystander effect was observed in the non-gene modified cells adjacent to the gene modified cells expressing tmpk-variants treated with nontoxic dose of AZT against the normal cells. GJIC formed between these cells are crucial to exhibit the effect. These results suggest that our tmpk/AZT approach is feasible to use for the anti-cancer gene therapy.

Research Products

• [Journal Article] Vascular endothelial growth factor broadens lentivector distribution in the heart after neonatal injection. (2009)
  • Author(s): Sato T, Ramsubir S, Higuchi K, Yanagisawa T, Medin JA.
  • Journal Title: J Cardiol 54(2) Pages: 245-254
  • Peer Reviewed
• [Journal Article] Direct injection of kit ligand-2 lentivirus improves cardiac repair and rescues mice post-myocardial infarction. (2009)
  • Author(s): Higuchi K, Ayach B, Sato T, Chen M, Devine SP, Rasaiah VI, Dawood F, Yanagisawa T, Tei C, Takenaka T, Liu PP, Medin JA.
  • Journal Title: Mol Ther. 17(2) Pages: 262-268
  • Peer Reviewed
• [Journal Article] CLIC4 interacts with histamine H3 receptor and enhances the receptor cell surface expression. (2008)
  • Author(s): Maeda K, Haraguchi M, Kuramasu A, Sato T, Ariake K, Sakagami H, Kondo H, Yanai K, Fukunaga K, Yanagisawa T, Sukegawa J.
  • Journal Title: Biochem Biophys Res Commun. 369(2) Pages: 603-608
  • Peer Reviewed
• [Journal Article] Potentiation of potassium currents by beta-adrenoceptor agonists in human urinary bladder smooth muscle cells : a possible electrical mechanism of relaxation. (2008)
  • Author(s): Takemoto J, Masumiya H, Nunoki K, Sato T, Nakagawa H, Ikeda Y, Arai Y, Yanagisawa T.
  • Journal Title: Pharmacology. 81(3) Pages: 251-258
  • Peer Reviewed
• [Presentation] Mechanism of the tmpk/AZT-system bystander effect ; cell fate control gene therapy for anti-cancer treatment. (2010)
  • Author(s): Takeya Sato
  • Organizer: 第13回アメリカ遺伝子細胞治療学会ジウム
  • Place of Presentation: ワシントンDCアメリカ
  • Year and Date: 20100519-20100522
• [Presentation] 新生マウスに対するVEGF前投与は遺伝子治療効果を改善する (2010)
  • Author(s): 佐藤岳哉
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20100316-20100318
• [Presentation] 抗ガン治療法としてのTmpk/AZT系におけるBystander効果発現の分子機構 (2010)
  • Author(s): 佐藤岳哉
  • Organizer: 第61回日本薬理学会北部会
  • Place of Presentation: 札幌
  • Year and Date: 2010-09-10
• [Presentation] Gap junction-mediated bystander killing highlights the utility of the TmpkF105Y/AZT system for cancer suicide gene therapy. (2009)
  • Author(s): Takeya Sato
  • Organizer: 第15回日本遺伝子治療学会年会
  • Place of Presentation: 大阪
  • Year and Date: 20090709-20090711
• [Presentation] 新規遺伝子治療法tmpk/AZT系の抗ガン治療法としての有効性の検証(In vivo Bystander効果の確認) (2009)
  • Author(s): 佐藤岳哉
  • Organizer: 第60回日本薬理学会北部会
  • Place of Presentation: 富山
  • Year and Date: 2009-09-26
• [Remarks] ホームページ等