2010 Fiscal Year Final Research Report
Development of Non-invasive Screening Tool to Detect Colorectal Neoplasia.
| Project/Area Number |
20590572
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama University |
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Principal Investigator |
NAGASAKA Takeshi Okayama University, 病院, 助教 (30452569)
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| Co-Investigator(Kenkyū-buntansha) |
KONDOU Yoshitaka 岡山大学, 病院, 医員 (50534765)
TANAKA Noriaki 岡山大学, 大学院・医歯薬学総合研究科, 教授 (10127566)
KOBAYASHI Naoya 岡山大学, 病院, 講師 (10325102)
MATSUBARA Nagahide 岡山大学, 医学部・歯学部附属病院, 講師 (70314672)
SASAMOTO Hiromi 岡山大学, 大学院・医歯薬学総合研究科, 外国人客員研究員 (10452567)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 腫瘍検査学 / 大腸癌 / メチル化 / スクリーニング / 便潜血反応 |
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| Research Abstract |
The development of noninvasive screening tests is important to reduce mortality from gastrointestinal neoplasia. We sought to develop such a test by analysis of DNA methylation from exfoliated cancer cells in feces. We developed a novel strategy that uses single-step modification of DNA with sodium bisulfite and fluorescence polymerase chain reaction methodology to measure aberrant methylation in fecal DNA. Methylation was analyzed in 296 fecal samples obtained from a variety of patients, including 21 with gastric tumors, 152 with colorectal tumors, and 10 with non-neoplastic or inflammatory lesions in the gastrointestinal lumen. The assay successfully identified one or more methylated markers in fecal DNA from 57.1% of patients with gastric cancer, 75.0% of patients with colorectal cancer, and 44.4% of patients with advanced colorectal adenomas, but only 10.6% of subjects without neoplastic or active diseases. Methylation in fecal DNA is associated with the presence of gastrointestinal tumors relative to non-neoplastic conditions. Our novel fecal DNA methylation assay provides a possible means to noninvasively screen not only for colorectal tumors but also for gastric tumors.
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[Journal Article] Analysis of fecal DNA methylation to detect gastrointestinal neoplasia.2009
Author(s)
Nagasaka T, Tanaka N, Cullings HM, Sun DS, Sasamoto H, Uchida T, Koi M, Nishida N, Naomoto Y, Boland CR, Matsubara N, Goel A.
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Journal Title
J Natl Cancer Inst. 101(18)
Pages: 1244-1258
Peer Reviewed
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[Journal Article] Mutations in both KRAS and BRAF may contribute to the methylator phenotype in colon cancer.2008
Author(s)
Nagasaka T, Koi M, Kloor M, Gebert J, Vilkin A, Nishida N, Shin SK, Sasamoto H, Tanaka N, Matsubara N, Boland CR, Goel A.
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Journal Title
Gastroenterology. 134(7)
Pages: 1950-1960
Peer Reviewed
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