2010 Fiscal Year Final Research Report

Improved function of ketoamine oxidase for clinical examination reagent of diabetes by use of multiple approaches

Research Project

Project/Area Number	20590579
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Laboratory medicine
Research Institution	Kyushu University
Principal Investigator	UEDA Tadashi Kyushu University, 薬学研究院, 教授 (90184928)
Co-Investigator(Kenkyū-buntansha)	OHKURI Takatoshi 九州大学, 大学院・薬学研究院, 助教 (70346807)
Co-Investigator(Renkei-kenkyūsha)	ABE Yoshito 九州大学, 大学院・薬学研究院, 准教授 (60315091) SHIOI Seijiro 福岡大学, RIセンター実験施設, 助教 (80423531)
Project Period (FY)	2008 – 2010
Keywords	糖尿病 / ケトアミンオキシダーゼ / アミノ酸改変 / X線結晶解析 / 安定化
Research Abstract	For prevention of diabetes, we should control of the concentration of glucose in our vessels. Since the concentration of glucose is affected by foods, the concentration of glycated proteins in vessels often became used as an indicator of the average of concentration of glucose. The ketoamine oxidase is crucial for the evaluation of the concentration of glucose from the glycated proteins. To improve the function of the ketoamine oxidase, we prepared many ketoamine oxidase mutants but did not obtain ketoamine oxidase mutant with improved function. Then, in order to obtain the basis for mutation design, we tried to analyze the three-dimensional structure of the ketoamine oxidase in vain.