A novel method of diagnosis for acute myocardial infarction

2011 Fiscal Year Final Research Report

• Project/Area Number: 20590679
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Niigata University (2009-2011); Shimane University (2008)
• Principal Investigator: TAKATSUKA Hisakazu 新潟大学, 医歯学系, 准教授 (40242490)
• Co-Investigator(Kenkyū-buntansha): TAKESHITA Haruo 島根大学, 医学部, 教授 (90292599) ; FUJIHARA Junko 島根大学, 医学部, 助教 (20346381)
• Co-Investigator(Renkei-kenkyūsha): YASUDA Toshihiro 福井大学, 医学部, 教授 (80175645)
• Project Period (FY): 2008 – 2011
• Keywords: 心筋梗塞 / DNase I / 司法解剖 / 法医学

Research Abstract

The aim of this study was confirm a molecular basis for utilization of Dnase I as a diagnostic marker death due to acute myocardial infarction; (1) SNP of Dnase I exon8, A2317G is the same tendency of Asians, and this SNP affect to heat sensitivity and optimum pH. (2) Detect of Dnase I activity within 10 minutes using microchip electrophoresis. (3) Unable to detect cytokines mRNA of postmortem cardiac tissue. (4) Unable to detect NT-pro-BNP using postmortem blood for novel diagnostic method. (5) SNP of Dnase I, type of 1-2 is the risk factor for myocardial infarction.

Research Products

• [Journal Article] Functional and genetic survey of all known the SNPs within human deoxyribonuclease I gene in wide-ranging ethnic groups (2011)
  • Author(s): Fujihara J, Ueki M, Yasuda T, Iida R, Soejima M, Koda Y, Kimura-Kataoka K,Kato H, Panduro A, Tongu M, Takeshita H
  • Journal Title: DNA and Cell Biol. Volume: 30(4) Pages: 205-217
  • DOI: DOI:10.1089/dna.2010.1120
  • Peer Reviewed
• [Journal Article] Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradationRapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation (2011)
  • Author(s): Fujihara J, Tabuchi M, Yasuda T, Fujita Y, Takeshita H
  • Journal Title: Anal Biochem Volume: 413(1) Pages: 78-79
  • DOI: DOI:10.1016/j.ab.2011.02.011
  • Peer Reviewed
• [Journal Article] A biochemical and genetic study on all non-synonymous single nucleotide polymorphisms of the gene encoding human deoxyribonuclease I potentially relevant to autoimmunity (2010)
  • Author(s): Yasuda T, Ueki M, Takeshita H, Fujihara J, Kimura-Kataoka K, Iida R, Tsubota E, Soejima M, Koda Y, Kato H, Panduro A
  • Journal Title: Int J Biochem Cell Biol. Volume: 42(7) Pages: 1216-1225
  • DOI: DOI:10.1016/j.biocel.2010.04.012
  • Peer Reviewed
• [Journal Article] Global analysis of single nucleotide polymorphisms in the exons of human deoxyribonuclease I-like 1 and 2 genes (2010)
  • Author(s): Fujihara J, Yasuda T, Iida R, Kimura-Kataoka K, Soejima M, Koda Y, Kato H, Panduro A, Yuasa I, Takeshita H
  • Journal Title: Electrophoresis Volume: 31(21) Pages: 3552-3557
  • DOI: DOI:10.1002/elps.201000319
  • Peer Reviewed
• [Journal Article] Gln222Arg (A2317G) polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences (2009)
  • Author(s): Takeshita H , Soejima M, Koda Y, Yasuda T, Takatsuka H, Fujihara J
  • Journal Title: Clin Chem Lab Med Volume: 47(1) Pages: 51-55
  • DOI: DOI:10.1515/CCLM.2009.002
  • Peer Reviewed
• [Journal Article] Serum deoxyribonuclease I can be used as a useful marker for diagnosis of death due to ischemic heart disease (2009)
  • Author(s): Yasuda T, Iida R, Kawai Y, Nakajima T, Kominato Y, Fujihara J, Takeshita H
  • Journal Title: Legal Med Volume: 11 Pages: S213-S215
  • DOI: DOI:10.1016/j.legalmed.2009.01.092
  • Peer Reviewed
• [Journal Article] Extremely high prevalence of DNASE1*1 allele in African populations (2008)
  • Author(s): Takeshita H, Fujihara J, Soejima M, Koda Y, Yasuda T, Nakajima T
  • Journal Title: Cell Biochem Funct Volume: 26 Pages: 151-153
  • DOI: DOI:10.1002/cbf.1414
  • Peer Reviewed
• [Presentation] 哺乳類DNase IにおけるN-グリコシド型糖鎖解 (2010)
  • Author(s): 藤原純子
  • Organizer: 第94次日本法医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24
• [Presentation] DNase I及びDNase II遺伝子exon内SNP検索:集団調査及び酵素活性に及ぼす変異の影響 (2010)
  • Author(s): 木村かおり
  • Organizer: 第94次日本法医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24
• [Presentation] サンドイッチELISA法による血清DNase I蛋白量の測定 (2010)
  • Author(s): 中島たみ子
  • Organizer: 第94次日本法医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24
• [Presentation] DNase I exon内非同義置換SNP検索:酵素性状に及ぼす置換の影響 (2009)
  • Author(s): 藤原純子
  • Organizer: 第26回日本法医学会中四国地方会
  • Place of Presentation: 山口
  • Year and Date: 2009-10-17
• [Presentation] DNase II遺伝子exon内SNP検索:酵素活性に及ぼす変異の影響 (2009)
  • Author(s): 木村かおり
  • Organizer: 第26回日本法医学会中四国地方会
  • Place of Presentation: 山口
  • Year and Date: 2009-10-17
• [Presentation] DNA修復酵素(hOGGおよびXRCC1)多型における酸化ストレスとの相関 (2009)
  • Author(s): 竹下治男
  • Organizer: 第93次日本法医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-05-15
• [Presentation] DNASE1 exon内SNP検索:アジア人においてDNase I Gln222Argのみが多型性を有する (2009)
  • Author(s): 藤原純子
  • Organizer: 第93次日本法医学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-05-14
• [Book] NA多型vol.16 (2009)
  • Author(s): 藤原純子, 竹下治男, 高塚尚和, 安田年博, 植木美鈴, 飯田礼子, 河合康幸, 中島たみ子, 小湊慶彦, 副島美貴子, 神田芳郎
  • Total Pages: 210-212
  • Publisher: 東洋書店