2010 Fiscal Year Final Research Report
Development of new molecular technique for determination of death by drowning
| Project/Area Number |
20590682
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | University of Miyazaki |
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Principal Investigator |
YUKAWA Nobuhiro University of Miyazaki, 医学部, 教授 (30240154)
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| Co-Investigator(Kenkyū-buntansha) |
KAKIZAKI Eiji 宮崎大学, 医学部, 助教 (70284833)
KOZAWA Shuji 宮崎大学, 医学部, 助教 (20379944)
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| Research Collaborator |
UCHIYAMA Taketo 宮崎大学, 大学院・医学系研究科, 大学院生
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| Project Period (FY) |
2008 – 2010
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| Keywords | 法医学 / 溺死 / 水棲細菌 / 分子生物学的手法 |
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| Research Abstract |
We determined some bacterioplankton as markers for diagnosis of drowning and tried to develop a new method using TaqMan-PCR : the genus Aeromomas (A.hydrophila, A.salmonicida), Vibrio (V.fischeri, V.harveyi, V.parahaemolyticus), Photobacterium (P.damselae, P.phosphoreum, P.leiognathi). We adopted the method of Triplex-PCR, which can detect three type of bacterioplankton simultaneously, and PCR was performed by 40 cycles using 7500 Real Time PCR System. Present method was specific for standard strains of above bacterioplankton. Moreover, bacterioplankton was detectable in blood or tissue from cadavers retrieved from the sea (n=5), rivers (fresh water, n=8),near estuaries (n=2) by the new method.
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