Molecular Screening by Microarray analysis for Hepatocarcinogenesis.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590752
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Hokkaido University
• Principal Investigator: CHUMA Makoto Hokkaido University, 病院, 助教 (30360910)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Takahiko 北海道大学, 大学院・医学研究科, 客員研究員 (80333607)
• Project Period (FY): 2008 – 2010
• Keywords: 癌進展 / RNA結合タンパク / RBM5

Research Abstract

The expression of the RBM5 mRNA is decreased in cancer tissues. Overexpression of RBM5 enhanced p53-mediated inhibition of cancer cell growth and colony formation. RBM5 affected p53 transcriptional activity and levels of mRNA and protein for endogenous p53 target genes. RBM5 influenced protein, but not mRNA, levels of endogenous p53 suggesting that RBM5 contributes to p53 activity through post-transcriptional mechanisms. Our results show that RBM5 contributes to p53 transcriptional activity, and that growth suppression and apoptosis mediated by RBM5 are linked to activity of the tumor suppressor protein p53.

Research Products

• [Journal Article] p53 transactivation is involved in the antiproliferative activity of the putative tumor suppressor RBM5. (2011)
  • Author(s): Kobayashi T, Ishida J, Musashi M, Ota S, Yoshida T, Shimizu Y, Chuma M, Kawakami H, Asaka M, Tanaka J, Imamura M, Kobayashi M, Itoh H, Edamatsu H, Sutherland LC, Brachmann RK.
  • Journal Title: Int J Cancer. 128(2) Pages: 304-318
  • Peer Reviewed
• [Journal Article] The influence of hepatitis B DNA level and antiviral therapy on recurrence after initial curative treatment in patients with hepatocellular carcinoma. (2009)
  • Author(s): Chuma M, Hige S, Kamiyama T, Meguro T, Nagasaka A, Nakanishi K, Yamamoto Y, Nakanishi M, Kohara T, Sho T, Yamamoto K, Horimoto H, Kobayashi T, Yokoo H, Matsushita M, Todo S, Asaka M.
  • Journal Title: J Gastroenterol. 44(9) Pages: 991-999
  • Peer Reviewed
• [Journal Article] 8-OHdG is a risk factor for development of hepatocellular carcinoma in patients with chronic hepatitis C virus infection. (2008)
  • Author(s): Chuma M, Hige S, Nakanishi M, Ogawa K, Natsuizaka M, Yamamoto Y, Asaka M.
  • Journal Title: J Gastroenterol Hepatol. 23(9) Pages: 1431-1436
  • Peer Reviewed
• [Presentation] The Influence of Hepatitis B DNA Level and Antiviral Therapy on Recurrence After Initial Curative Treatment in Patients With Hepatocellular Carcinoma. (2009)
  • Author(s): Chuma M.
  • Organizer: 第19回 アジア太平洋肝臓学会
  • Place of Presentation: 香港
  • Year and Date: 2009-02-15
• [Presentation] 8-OHdG is a risk factor for development of hepatocellular carcinoma in patients with chronic hepatitis C virus infection. (2008)
  • Author(s): Chuma M.
  • Organizer: 第43回欧州肝臓学会
  • Place of Presentation: ミラノ
  • Year and Date: 2008-04-25