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2010 Fiscal Year Final Research Report

Analysis of biological characteristic features in cancer stem cell in digestive organs

Research Project

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Project/Area Number 20590809
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKeio University

Principal Investigator

HIGUCHI Hajime  Keio University, 医学部, 助教 (20306682)

Project Period (FY) 2008 – 2010
Keywords癌幹細胞 / epithelial-to-mesenchymal transition / apoptosis / mesenchymal stem cell
Research Abstract

Cancer related materials, i.e., cell lines, endoscopic biopthy specimen, or, surgically resected specimen, were analyzed for ; i) expression of cancer stem cell markers, ii) tumor formation, iii) colony forming activity, iv) drug-resistance, and v) potentials for local invasion and metastasis. Our results suggest that side population (SP) cells isolated from pancreatic cancer have some cancer stem cell-like properties ; i) high tumorigenic/colony forming activity, ii) resistance to some chemotherapeutic drugs as well as apoptosis-inducing molecules such as TRAIL, iii) high metstatic potential, and iv) enhanced capability to undergo epithelial-to-mesenchymal transition (EMT) and invasion. In hepatocellular carcinoma, CD133-positive side population cells were highly tumorigenic and chemotherapy-resistant. These cancer stem cell-like features were enhanced by co-culturing with bone marrow-derived mesenchymal stem cells (MSCs) and/or MSC-derived myofibroblast-like cells, suggesting MSCs provide microenvironment so-called cancer stem cell niche to maintain stem cell phenotype in cancer derived SP cells. These results suggest that SP cells would be a therapeutic tagrget, especially to prevent cancer sten cell invasion and metastasis. Mesenchymal cell-derived microenvironment would be another terapeutic target to regulate cancer stem cell maintenance.

  • Research Products

    (7 results)

All 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article]2010

    • Author(s)
      Satoshi Kurita, Hajime Higuchi, Yoshimasa Saito, Nobuhiro Nakamoto, Hiromasa Takaishi, Shinichiro Tada, Hidetsugu Saito, Gregory J. Gores, Toshifumi Hibi
    • Journal Title

      Cancer Sci 101

      Pages: 1431-1439

    • Peer Reviewed
  • [Journal Article] Side population of pancreatic cancer cells predominates in TGF-beta-mediated epithelial to mesenchymal transition and invasion.2009

    • Author(s)
      Ayano Kabashima, Hajime Higuchi, Hiromasa Takaishi, Yumi Matsuzaki, Sadafumi Suzuki, Motoko Izumiya, Hideko Iizuka, Gen Sakai, Shigenari Hozawa, Toshifumi Azuma, Toshifumi Hibi
    • Journal Title

      Int-J-Cancer 124

      Pages: 2771-2779

    • Peer Reviewed
  • [Presentation] Wnt signaling contributes to the maintenance of cancer stem cell-like populations in Huh7 hepatocellular carcinoma cell line : Role of hepatic stellate cells.2010

    • Author(s)
      Masayuki Adachi, Hajime Higuchi, Ayano Kabashima, Shinsuke Funakoshi, Shoko Nakamura, Hiromasa Takaishi, Toshifumi Hibi
    • Organizer
      AACR meeting abstract 2010, 203
    • Place of Presentation
      Washington
    • Year and Date
      20100417-20100421
  • [Presentation] Cancer stem cell like properties of 5-fluorouracil resistant Panc2010

    • Author(s)
      Motoko Izumiya, Hajime Higuchi, Ayano Kabashima, Toru Igarashi, Shoko Nakamura, Shinsuke Funakoshi, Masayuki Adachi, Hiromasa Takaishi, Toshifumi Hibi
    • Organizer
      AACR meeting abstract 2010, 335 101^<st> American Association for Cancer Research
    • Place of Presentation
      Washington
    • Year and Date
      20100417-20100421
  • [Presentation] Mesenchymal stem cells induce epithelial-to-mesenchymal transition (EMT) in metastatic SP cells in pancreatic cancer.2010

    • Author(s)
      Ayano Kabashima, Hajime Higuchi, Yumi Matsuzaki, Shinsuke Funakoshi, Masayuki Adachi, Motoko Izumiya, Gen Sakai, Toru Igarashi, Shoko Nakamura, Hiromasa Takaishi, Toshifumi Hibi
    • Organizer
      AACR meeting abstract 2010, 387 101st American Association for Cancer Research
    • Place of Presentation
      Washington
    • Year and Date
      20100417-20100421
  • [Presentation] Isolation and identification of Notch signal positive cells in pancreatic cancer cell line.2009

    • Author(s)
      Gen Sakai, Hajime Higuchi, Hiromasa Takaishi, Motoko Izumiya, Masaya Adachi, Ayano Kabashima, Shouko Nakamura, Toshifumi Hibi
    • Organizer
      AACR meeting abstract 2009, 193 100^<th> American Association for Cancer Research.
    • Place of Presentation
      Denver
    • Year and Date
      20090418-20090422
  • [Remarks] ホームページ等

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Published: 2012-01-26   Modified: 2016-04-21  

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