Crosstalk between mitochondria and nucleus : signaling via inositol-3-phosphate receptor

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590858
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: HAYASHI Hideharu Hamamatsu University School of Medicine, 医学部, 教授 (50135258)
• Co-Investigator(Kenkyū-buntansha): SATOH Hiroshi 浜松医科大学, 医学部附属病院, 講師 (30293632) ; KATOH Hideki 浜松医科大学, 医学部, 助教 (80314029)
• Project Period (FY): 2008 – 2010
• Keywords: ミトコンドリア / 共焦点レーザー顕微鏡 / 細胞内Ca^<2+> / ミトコンドリア膜透過性遷移孔 / 膜電位 / カルモジュリン / glycogen synthase kinase-3β / hexokinase II

Research Abstract

Mitochondrial PTP opened and ΔΨm depolarized by CaM/CaMKII via the production of reactive oxygen species. These effects were regulated by an elevation of Ca^<2+> released from the saroplasmic reticulum, showing the cross-talk between SR and mitochondria. An elevation of Ca^<2+> could also regulate the nucelar transcription factor. Opening of mPTP by GSK3β was shown to be dependent on the binding of hexokinase II, which explains one mechanism of signaling transduction in mitochondria.

Research Products

• [Journal Article] Comparison of global and regional abnormalities in ^<99m>Tc-sestamibi and cardiac magnetic resonance imaging in dilated cardimyopathy. (2010)
  • Author(s): Shiraki K, Satoh H, Saitoh T, Saotome M, Urushida T, Katoh H, Takehara Y, Sakahara H, Hayashi H.
  • Journal Title: J.Cardiac.Fail. 16 Pages: 641-648
• [Journal Article] Delayed enhancement on cardiac magnetic resonance and clinical, morphological and electrocardiographical features in hypertrophic cardiomyopathy. (2009)
  • Author(s): Satoh H, Matoh F, Shiraki K, Saitoh T, Odagiri K, Saotome M, Usushida T, Katoh H, Takehara Y, Sakahara H, Hayashi H.
  • Journal Title: J.Cardiac.Fail. 15 Pages: 419-427
• [Journal Article] Local control of mitochondrial membrane potential, permeability transition pore and reactive oxygen species by calcium and calmodulin in rat ventricular myocytes. (2009)
  • Author(s): Odagiri K, Katoh H, Kawashima H, Tanaka T, Ohtani H, Saotome M, Urushida T, Satoh H, Hayashi H.
  • Journal Title: J.Mol.Cell.Cardiol. 46 Pages: 989-997
• [Journal Article] Transient opening of mitochondrial permeability transition pore by reactive oxygen species protects myocardium from ischemia/reperfusion injury. (2009)
  • Author(s): Saotome M, Katoh H, Yaguchi Y, Tanaka T, Urushida T, Satoh H, Hayashi H.
  • Journal Title: Am.J.Physiol.Heart Circ.Physiol. 296 Pages: H1125-H1132
• [Journal Article] Protein phosphatase inhibitor-1 augments a protein kinase A-dependent increase in the Ca^<2+> loading of the sarcoplasmic reticulum without changing its Ca^<2+> release. (2009)
  • Author(s): Kawashima H, Satoh H, Saotome M, Urushida T, Katoh H, Hayashi H.
  • Journal Title: Circ.J. 73 Pages: 1133-1140
• [Journal Article] Extracellular acidosis suppresses endothelial function by inhibiting store-operated Ca^<2+> entry via non-selective cation channels. (2009)
  • Author(s): Asai M, Takeuchi K, Saotome M, Urushida T, Katoh H, Satoh H, Hayashi H, Watanabe H.
  • Journal Title: Cardiovasc.Res. 83 Pages: 97-105
• [Journal Article] Misinterpretation of the effect of amlodipine on cytosolic calcium concentration with fura-2 fluorospectrometry. (2008)
  • Author(s): Asai M, Takeuchi K, Uchida S, Urushida T, Katoh H, Satoh H, Yamada S, Hayashi H, Watanabe H
  • Journal Title: Naunyn-Schmiedeberg's Arch.Pharmacol. 377 Pages: 423-427
• [Journal Article] The usefulness of delayed enhancement magnetic resonance imaging for diagnosis and evaluation of cardiac function in patients with cardiac sarcoidosis. (2008)
  • Author(s): Matoh F, Satoh H, Shiraki K, Odagiri K, Saitoh T, Urushida T, Katoh H, Takehara Y, Sakahara H, Hayashi H.
  • Journal Title: J.Cardiol. 51 Pages: 179-188
• [Journal Article] Different effects of palmitoyl-L-carnitine and palmitoyl-CoA on mitochondrial function in rat ventricular myocytes. (2008)
  • Author(s): Tominaga H, Katoh H, Odagiri K, Takeuchi Y, Kawashima H, Saotome M, Urushida T, Satoh H, Hayashi H.
  • Journal Title: Am.J.Physiol.Heart Circ.Physiol. 295 Pages: H105-H112
• [Presentation] Binding of Hexokinase to Mitochondria is Involved in the Regulation of Mitochondrial Permeability Transition Pore by Glycogen Synthase Kinase-3β. (2010)
  • Author(s): Katoh H, Kumazawa A, Tanaka T, Nobuhara M, Otani H, Saotome M, Urushida T, Satoh H, Hayashi H
  • Organizer: XX World Congress of the International Society for Heart Research
  • Place of Presentation: Kyoto
  • Year and Date: 20100500
• [Presentation] Protein phosphatase inhibitor-1 can augment a protein kinase A-dependent increase in the SR Ca^<2+> loading without changing the SR Ca^<2+> release. (2010)
  • Author(s): Satoh H, Kawashima H, Saotome M, Katoh H, Hayashi H
  • Organizer: The 20th World Congress of International Society of Heart Research
  • Place of Presentation: Kyoto.
  • Year and Date: 20100500
• [Presentation] Protein phosphatase inhibitor 1 (I-1) augments protein kinase A-dependent increase in SR Ca^<2+> loading without changing SR Ca^<2+> release events. (2008)
  • Author(s): Kawashima H, Satoh H, Katoh H, Hayashi H
  • Organizer: The 72th Annual Meeting of Japanese Circulation Society.
  • Place of Presentation: Fukuoka.
  • Year and Date: 20080300
• [Presentation] Local control of mitochondrial membrane potential and permeability transition pore by calcium and calmodulin in rat ventricular myocytes. (2008)
  • Author(s): Odagiri K, Katoh H, Tominaga H, Kawashima H, Takeuchi Y, Tanaka T, Ohtani H, Urushida T, Satoh H, Hayashi H
  • Organizer: The 72th Annual Meeting of Japanese Circulation Society.
  • Place of Presentation: Fukuoka.
  • Year and Date: 20080300
• [Presentation] Dual effects of nitric oxide on mitochondrial permeability transition in rat ventricular myocytes. (2008)
  • Author(s): Otani H, Katoh H, Tominaga H, Odagiri K, Takamitsu Y, Tanaka T, Urushid T, Satoh H, Hayashi H
  • Organizer: The 72th Annual Scientific Meeting of the Japanese Circulation Society.
  • Place of Presentation: Fukuoka.
  • Year and Date: 20080300
• [Book] 再灌流障害.循環器疾患最新の治療.2010-2011 (2010)
  • Author(s): 林秀晴
  • Total Pages: 123-125
  • Publisher: 南江堂