Identification and characterization of proteins bound to MURC which is involved in cardiac dysfunction and arrhythmia

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590863
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: UEYAMA Tomomi Kyoto Prefectural University of Medicine, 医学研究科, 講師 (80379388)
• Project Period (FY): 2008 – 2010
• Keywords: 心不全 / 不整脈 / 筋特異的遺伝子 / 結合蛋白質 / MURC

Research Abstract

We found that MURC was localized to caveolae at the plasma membrane of cardiomyocytes, and that MURC was associated with caveolin-3, PTRF/cavin-1, SDPR/cavin-2, and SRBC/cavin-3. We also found that overexpression of MURC in cardiomyocytes induced distension of caveolae and transgenic mice expressing MURC in the heart exhibited deformation of caveolae in cardiomyocytes.

Research Products

• [Journal Article] PARM-1 is an endoplasmic reticulum molecule involved in endoplasmic reticulum stress-induced apoptosis in rat cardiac myocytes. (2010)
  • Author(s): Isodono K, Takahashi T, Imoto H, Nakanishi N, Ogata T, Asada S, Adachi A, Ueyama T, Oh H, Matsubara H.
  • Journal Title: PLoS ONE. 5 Pages: e9746
  • Peer Reviewed
• [Journal Article] Deficiency of nectin-2 leads to cardiac fibrosis and dysfunction under chronic pressure overload. (2009)
  • Author(s): Satomi-Kobayashi S, Ueyama T, Mueller S, Toh R, Masano T, Sakoda T, Rikitake Y, Miyoshi J, Matsubara H, Oh H, Kawashima S, Hirata K, Takai Y.
  • Journal Title: Hypertension 54 Pages: 825-831
  • Peer Reviewed
• [Journal Article] Role of endothelial lipase in plasma HDL levels in a murine model of hypertriglyceridemia. (2009)
  • Author(s): Tanaka H, Ishida T, Johnston TP, Yasuda T, Ueyama T, Kojima Y, Kundu RK, Quertermous T, Ishikawa Y, Hirata K.
  • Journal Title: J Atheroscler Thromb. 16 Pages: 327-338
  • Peer Reviewed
• [Journal Article] Skeletal muscle-derived progenitors capable of differentiating into cardiomyocytes proliferate through myostatin- independent TGF-βfamily signaling. (2008)
  • Author(s): Nomura T, Ueyama T, Ashihara E, Tateishi K, Asada S, Nakajima N, Isodono K, Takahashi T, Matsubara H, Oh H.
  • Journal Title: Biochem Biophys Res Commun. 365 Pages: 863-869
  • Peer Reviewed
• [Journal Article] MURC, a muscle-restricted coiled-coil protein that modulates the Rho/ROCK pathway, induces cardiac dysfunction and conduction disturbance. (2008)
  • Author(s): Ogata T, Ueyama T, Isodono K, Tagawa M, Takehara N, Kawashima T, Takahashi T, Harada K, Shioi T, Matsubara H, Oh H.
  • Journal Title: Mol Cell Biol. 28 Pages: 3424-3436
  • Peer Reviewed
• [Journal Article] MURC, a muscle-restricted coiled-coil protein, is involved in the regulation of skeletal myogenesis. (2008)
  • Author(s): Tagawa M, Ueyama T, Ogata T, Takehara N, Nakajima N, Isodono K, Asada S, Takahashi T, Matsubara H, Oh H.
  • Journal Title: Am J Physiol-Cell Physiol 295 Pages: C490-C498
  • Peer Reviewed
• [Journal Article] Controlled delivery of basic fibroblast growth factor promotes human cardiosphere-derived cell engraftment to enhance cardiac repair for chronic myocardial infarction. (2008)
  • Author(s): Takehara N, Tsutsumi Y, Tateishi K, Ogata T, Tanaka H, Ueyama T, Takahashi T, Takamatsu T, Fukushima M, Komeda M, Yamagishi M, Yaku H, Tabata Y, Matsubara H, Oh H.
  • Journal Title: J Am Coll Cardiol. 52 Pages: 1858-1865
  • Peer Reviewed
• [Presentation] MURC, muscle-restricted coiled- coil protein, regulates caveolae morphology and induces hypertrophy in cardiomyocytes. (2010)
  • Author(s): Taniguchi T, Ogata T, Naito D, Nakanishi N, Miyagawa K, Amano K, Isodono K, Imoto H, Tagawa M, Takehara N, Asada S, Adachi A, Morimoto T, Takahashi T, Oh H, Ueyama T, Matsubara H
  • Organizer: American Heart Association (米国心臓病学術会議)
  • Place of Presentation: Chicago, USA
  • Year and Date: 20101113-20101117
• [Presentation] Cavin familyとしてのMURCの役割およびMURCの心筋疾患に及ぼす影響とメカニズムの検討 (2010)
  • Author(s): 中西直彦、小形岳寛、宮川浩太郎、内藤大督、谷口琢也、天野克也、上山知己、松原弘明
  • Organizer: 第33回日本高血圧学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20101000
• [Presentation] PTRF欠損症の臨床病態に関する研究 (2010)
  • Author(s): 林由起子、松田知栄、小川恵、上山知己、小形岳寛、天野克也、松原弘明、埜中征哉、西野一三
  • Organizer: 第51回日本神経学会総会
  • Place of Presentation: 東京
  • Year and Date: 20100500
• [Presentation] MURC induces cardiomyocyte hypertrophy through the extracellular signal-regulated kinase pathway. (2010)
  • Author(s): Naito D, Ogata T, Amano K, Taniguchi T, Isodono K, Adachi A, Imoto H, Nakanishi N, Kangawa H, Takahashi T, Ueyama T, Matsubara H
  • Organizer: International Symposium on Cardiovascular Endocrinology and Metabolism
  • Place of Presentation: Nara, Japan
  • Year and Date: 20100331-20100401
• [Presentation] NFAT5 is essential for mesodermal and cardiomyogenic differentiation through regulating the canonical pathway. (2010)
  • Author(s): Adachi A, Takahashi T, Imoto H, Nakanishi N, Kangawa H, Ueyama T, Matsubara H
  • Organizer: 第74回日本循環器学会総会
  • Place of Presentation: 京都
  • Year and Date: 20100300
• [Presentation] Serglycin, a novel adipokine highly expressed in epicardial adipose tissue. (2010)
  • Author(s): Imoto H, Takahashi T, Adachi A, Nakanishi N, Kangawa H, Ueyama T, Matsubara H
  • Organizer: 第74回日本循環器学会総会
  • Place of Presentation: 京都
  • Year and Date: 20100300
• [Presentation] PARM-1, a novel transmembrane molecule involved in endoplasmic reticulum stress-induced apoptosis in rat cardiac myocytes. (2010)
  • Author(s): Isodono K, Takahashi T, Imoto H, Nakanishi N, Ogata T, Asada S, Adachi A, Kangawa H, Ueyama T, Matsubara H
  • Organizer: 第74回日本循環器学会総会
  • Place of Presentation: 京都
  • Year and Date: 20100300
• [Presentation] Downregulation of Dicer Expression by Serum Withdrawal Sensitizes Human Endothelial Cells to Apoptosis. (2009)
  • Author(s): Asada S, Takahashi T, Isodono K, Imoto H, Adachi A, Ogata T, Ueyama T, Matsubara H, Oh H.
  • Organizer: 第73回日本循環器学会総会
  • Place of Presentation: 大阪
  • Year and Date: 20090300
• [Presentation] MURC, a muscle-restricted coiled-coil protein, induces cardiomyocyte hypertrophy through the extracellular signal-regulated kinase pathway. (2008)
  • Author(s): Ogata T, Ueyama T, Isodono K, Imoto H, Tagawa M, Takehara N, Asada S, Adachi A, Takahashi T, Matsubara Hiroaki, Oh H.
  • Organizer: American Heart Association Basic Cardiovascular Sciences Conference
  • Place of Presentation: Keystone, USA
  • Year and Date: 20080728-20080731
• [Presentation] A novel muscle-restricted coiled-coil protein, MURC, induces cardiac dysfunction accompanied with an altered gene expression profile. (2008)
  • Author(s): Ogata T, Ueyama T, Isodono K, Tagawa M, Takehara N, Kawashima T, Shioi T, Takahashi T, Matsubara Hiroaki, Oh H.
  • Organizer: 第72回日本循環器学会
  • Place of Presentation: 福岡
  • Year and Date: 20080300
• [Presentation] A novel but evolutionarily conserved muscle-restricted coiled-coil protein, MURC, regulates myogenic differentiation in C2C12 cells. (2008)
  • Author(s): Tagawa M, Ueyama T, Ogata T, Nakajima N, Isodono K, Asada S, Takehara N, Takahashi T, Matsubara Hiroaki, Oh H.
  • Organizer: 第72回日本循環器学会
  • Place of Presentation: 福岡
  • Year and Date: 20080300