Analysis of ARIA that is a novel gene regulating endothelial apoptosis in the regulation of neovessel formation

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590885
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: IKEDA Koji Kyoto Prefectural University of Medicine, 医学研究科, 助教 (90423871)
• Co-Investigator(Renkei-kenkyūsha): MATSUBARA Hiroaki 京都府立医科大学, 医学研究科, 教授 (10239072)
• Project Period (FY): 2008 – 2010
• Keywords: 分子血管病態学

Research Abstract

We have generated ARIA (former BLADE) knockout mice, and analyzed the ARIA function in the regulation of neovessel formation. By using the hind-limb ischemia model, we revealed that genetic deletion of ARIA substantiallyenhanced the ischemia-induced neovascularization. Moreover, we found that ARIA is highly expressed in endothelial progenitor cells as well, and regulates their angiogenic functions by modulating the PI3K/Akt pathway.

Research Products

• [Journal Article] Apoptosis regulator through modulating IAP expression (ARIA) regulates the PI3K/Akt pathway in endothelial and endothelial progenitor cells. (2011)
  • Author(s): Koide M, Ikeda K (corresponding author), Akakabe Y, Kitamura Y, Ueyama T, Matoba S, Yamada H, Okigaki M, Matsubara H.
  • Journal Title: Proc Natl Acad Sci USA in press
• [Journal Article] Endothelial FGF receptor signaling accelerates atherosclerosis. (2011)
  • Author(s): Che J, Okigaki M, Takahashi T, Katsume A, Adachi Y, Yamaguchi S, Matsunaga S, Takeda M, Matsui A, Kishita E, Ikeda K, Yamada H, Matsubara H.
  • Journal Title: Am J Physiol Heart Circ Physiol. 300(1) Pages: H151-H161
• [Journal Article] p53 and TIGAR regulate cardiac myocyte energy homeostasis under hypoxic condition. (2010)
  • Author(s): Kimata M, Matoba S, Iwai-Kanai E, Nakamura H, Hoshino A, Nakaoka M, Katamura M, Okawa Y, Mita Y, Okigaki M, Ikeda K, Tatsumi T, Matsubara H.
  • Journal Title: Am J Physiol Heart Circ Physiol. 299(6) Pages: H1908-H1916
• [Journal Article] Paracrine osteogenic signals via bone morphogenetic protein-2 accelerate the atherosclerotic intimal calcification in vivo. (2010)
  • Author(s): Nakagawa Y, Ikeda K (corresponding author), Akakabe Y, Koide M, Uraoka M, Yutaka KT, Kurimoto-Nakano R, Takahashi T, Matoba S, Yamada H, Okigaki M, Matsubara H.
  • Journal Title: Arterioscler Thromb Vasc Biol. 30(10) Pages: 1908-1915
• [Journal Article] Pathophysiology of vascular calcification : pivotal role of cellular senescence in vascular smooth muscle cells. (2010)
  • Author(s): Burton DG, Matsubara H, Ikeda K (last author).
  • Journal Title: Exp Gerontol. 45(11) Pages: 819-824
• [Journal Article] Bone marrow AT1 augments neointimal formation by promoting mobilization of smooth muscle progenitors via platelet-derived SDF-1alpha. (2010)
  • Author(s): Yokoi H, Yamada H, Tsubakimoto Y, Takata H, Kawahito H, Kishida S, Kato T, Matsui A, Hirai H, Ashihara E, Maekawa T, Iwai M, Horiuchi M, Ikeda K, Takahashi T, Okigaki M, Matsubara H.
  • Journal Title: Arterioscler Thromb Vasc Biol. 30(1) Pages: 60-67
• [Presentation] Identification of ARIA as a novel factor regulating endothelial apoptosis and angiogenesis in vivo. (2010)
  • Author(s): Koide M, Ikeda K, Akakabe Y, Uraoka M, Nakagawa Y, Kurimoto R, Matsubara H.
  • Organizer: 心血管内分泌学会
  • Place of Presentation: 奈良
  • Year and Date: 20100400
• [Presentation] Identification of ARIA as a novel factor regulating endothelial cell apoptosis and angiogenesis in vivo. (2010)
  • Author(s): Koide M, Ikeda K, Nakagawa Y, Akakabe Y, Kurimoto R, Matsubara H.
  • Organizer: 第74回日本循環器学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 20100300
• [Presentation] ARIA regulates ischemia-induced angiogenesis and vasculogenesis through modulating the PI3K/Akt/eNOS signaling pathway. (2010)
  • Author(s): Koide M, Ikeda K, Kitamura Y, Akakabe Y, Nakagawa Y, Matsubara H.
  • Organizer: AHA scientific sessions, Circulation 122(23) : A-14736
  • Year and Date: 20100000
• [Presentation] Identification of ARIA as a novel factor regulating endothelial cell apoptosis and angiogenesis in vivo. (2009)
  • Author(s): Masahiro Koide, Koji Ikeda, Maki Uraoka, Yusuke Nakagawa, Yoshiki Akakabe, Ritsuko Kurimoto, Hiroaki Matsubara.
  • Organizer: American Heart Association(米国心臓病学術会議)
  • Place of Presentation: Orlando, USA
  • Year and Date: 20091100
• [Book] 血管新生療法(循環器疾患-疾患モデルの作成と利用)第2章9節 (2010)
  • Author(s): 池田宏二, 他
  • Total Pages: 190-194
  • Publisher: 株式会社エル・アイ・シー
• [Remarks] ホームページ等
  • URL: http://www.f.kpu-m.ac.jp/k/med2/index.html
• [Patent(Industrial Property Rights)] 肥満・インスリン感受性を制御する遺伝子およびそのノックアウト動物および本意電子の機能調節を介した肥満及び/又はメタボリック症候群の予防及び/又は治療薬のスクリーニング (2011)
  • Inventor(s): 池田宏二、松原弘明、赤壁佳樹
  • Industrial Property Rights Holder: 池田宏二、松原弘明、赤壁佳樹
  • Industrial Property Number: 特許,特願2011-9375
  • Filing Date: 2011-01-20