2010 Fiscal Year Final Research Report
Identification of novel molecular targets for lung cancer based on different sensitivity to AP-1 blockade
| Project/Area Number |
20590909
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
KINOSHITA Ichiro Hokkaido University, 大学院・医学研究科, 講師 (40343008)
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| Co-Investigator(Renkei-kenkyūsha) |
AKITA Hirotoshi 北海道大学, 大学院・医学研究科, 教授 (70222528)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 肺癌 / 分子標的 / AP-1 / MCM4 / マイクロアレイ |
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| Research Abstract |
We identified 27 genes suppressed by blockade of AP-1, an oncogenic transcription factor, in lung cancer cells sensitive to the AP-1 blockade using microarray analysis. Among them, knockdown of MCM 4, a component of DNA replication licensing complex, by siRNA reduced cell growth in multiple lung cancer cells. Immunohistochemical analysis in surgically resected lung cancers demonstrated that MCM4 expression was correlated with proliferation markers including Ki-67. MCM4 may play an important role for growth of lung cancers and be a novel molecular target for lung cancer.
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