2010 Fiscal Year Final Research Report
Fundamental study of development of new antibiotics using defensins
| Project/Area Number |
20590927
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
MUKAE Hiroshi Nagasaki University, 医学部, 教授 (80253821)
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| Project Period (FY) |
2008 – 2010
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| Keywords | デフェンシン / プレクタシン / サイトカイン / 増殖因子 / 気道上皮細胞 / 抗菌活性 |
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| Research Abstract |
1. Plectasin is the first defensin-like peptide to be isolated from fungus, which has specific antibacterial activity against Streptococcus pneumoniae. In addition, it showed no cytotoxicity to human cells, and it did not induce IL-8 production in A549 cells. This suggests that plectasin could be an inoffensive alternative antibiotic for clinical application. 2. Human α-defensins contribute to excessive mucus production by increasing the production of MUC5AC from bronchial epithelial cells. Macrolide antibiotics directly inhibit these actions. 3. Human α-defensins mainly enhanced the expression of IL-8 in epithelial cells, whereas it enhanced transforming growth factor-β and vascular endothelial growth factor expressions in lung fibroblasts. These results suggest that humanα-defensins play different roles in the pathogenesis of idiopathic pulmonary fibrosis and diffuse panbronchiolitis according to the location in the lung where the humanα-defensins are mainly produced. It is possible that these effects of humanα-defensins carry a risk of adverse effects. Therefore we should examine the possibility of fungal defensins like plectasin as antibiotics.
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[Journal Article] Differential effects of human neutrophil peptide-1 on growth factor and interleukin-8 production by human lung fibroblasts and epithelial cells2010
Author(s)
Amenomori M, Mukae H, Ishimatsu Y, Sakamoto N, Kakugawa T, Hara A, Hara S, Fujita H, Ishimoto H, Hayashi T, Kohno S
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Journal Title
Exp Lung Res 36巻
Pages: 411-419
Peer Reviewed
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[Journal Article] Different effects of telithromycin on MUC5AC production induced by human neutrophil peptide-1 or lipopolysaccharide in NCI-H292 cells compared with azithromycin and clarithromycin2009
Author(s)
Ishimoto H, Mukae H, Sakamoto N, Amenomori M, Kitazaki T, Imamura Y, Fujita H, Ishii H, Nakayama S, Yanagihara K, Kohno S
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Journal Title
J Antimicrob Chemother 63巻
Pages: 109-114
Peer Reviewed
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