2010 Fiscal Year Final Research Report

Molecular mechanisms coordinating between cell cycle and differentiation in lung epithelial cells

Research Project

Project/Area Number	20590928
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Respiratory organ internal medicine
Research Institution	Kumamoto University
Principal Investigator	FUJIMOTO Tetsuhiro Kumamoto University, 大学院・生命科学研究部, 助教 (70359818)
Co-Investigator(Kenkyū-buntansha)	ITO Takaaki 熊本大学, 大学院・生命科学研究部, 教授 (70168392)
Project Period (FY)	2008 – 2010
Keywords	細胞周期 / 細胞癌化 / cyclin D1 / RUNX3 / p21
Research Abstract	Transcriptional function of cyclin D1, whose deregulation is frequently observed in human cancers, has been suggested to contribute to cancer formation. We show that cyclin D1 protein inhibits RUNX3 activity by directly binding to it andB interfering with its interaction with p300 interaction in lung cancer cells. Cyclin D1 inhibits p300-dependent RUNX3 acetylation and negatively regulates cyclin-dependent kinase (cdk) inhibitor p21 expression. These transcriptional effects of cyclin D1 do not require cdk4/6 kinase activation. We propose that cyclin D1 provides a transcriptional switch that allows the tumor suppressor activity of RUNX3 to be repressed in cancer cells.

(3 results)