The host defensive role of mast cells against airway viral infection.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590934
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Teikyo University
• Principal Investigator: NAGASE Hiroyuki Teikyo University, 医学部, 准教授 (40365945)
• Co-Investigator(Kenkyū-buntansha): OHTA Ken 帝京大学, 医学部, 教授 (30160500)
• Project Period (FY): 2008 – 2010
• Keywords: マスト細胞 / ウイルス / 二重鎖RNA / 気道感染 / 気管支喘息

Research Abstract

Mast cells have long been postulated to have a central role in the pathogenesis of allergic disease. In addition, it has been recently reported that mast cells also have a host defensive role against bacterial infection. But the role of mast cells against virus infection has not been clarified. The aim of the present study is to investigate the role of mast cells in host defense against viral infection. We intranasally inoculated poly I:C, which is an analogue compound of virus double-stranded RNA, into wild type or mast cell deficient W/Wv mice. Poly I : C induced lung inflammation in wild type mice, but the inflammation was diminished in mast cell deficient mice, and intravenous transfer of mast cells into mast cell deficient mice restored the inflammation. In addition, the local concentration of various chemokines and cytokines decreased in mast cell deficient mice. From those results, it was suggested that mast cells have a host-defensive role against viral infection via up-regulation of local cytokine and chemokine generation. This finding might contribute to clarify the mechanism of virus-induced exacerbation of allergic diseases including asthma.

Research Products

• [Journal Article] Leptin enhances survival and induces migration, degranulation, and cytokine synthesis of human basophils (2011)
  • Author(s): Suzukawa M, Nagase H, Ogahara I, Han K, Tashimo H, Shibui A, Koketsu R, Nakae S, Yamaguchi M, Ohta K
  • Journal Title: J Immunol 186(9) Pages: 5254-5260
  • Peer Reviewed
• [Journal Article] Reference ranges for exhaled nitric oxide fraction in healthy Japanese adult population (2010)
  • Author(s): Matsunaga K, Hirano T, Kawayama T, Tsuburai T, Nagase H, Aizawa H, Akiyama K, Ohta K, Ichinose M.
  • Journal Title: Allergol Int 59(4) Pages: 363-367
  • Peer Reviewed
• [Journal Article] Effect of tiotropium brmide on airway inflammation and remodelling in a mouse model of asthma (2010)
  • Author(s): Ohta S, Oda N, Yokoe T, Tanaka A, Yamamoto Y, Watanabe Y, Minoguchi K, Ohnishi T, Hirose T, Nagase H, Ohta K, Adachi M
  • Journal Title: Clin Exp Allergy 40(8) Pages: 1266-1275
  • Peer Reviewed
• [Journal Article] Intracellular protein phosphorylation in eosinophils and the functional relevance in cytokine production (2009)
  • Author(s): Yamamura K, Adachi T, Masuda T, Kojima Y, Hara A, Toda T, Nagase H, Ohta K
  • Journal Title: Int Arch Allergy Immunol 149(S1) Pages: 45-50
  • Peer Reviewed
• [Journal Article] アレルギー性気管支肺アスペルギルス症に続発した肺膿瘍が穿破し膿胸気管支瘻を呈した1例 (2009)
  • Author(s): 原麻恵,長瀬洋之,林秀敏,倉持美知雄,石田博文,足立哲也,鈴木直仁,大田健
  • Journal Title: 日本呼吸器学会雑誌 47 Pages: 432-437
  • Peer Reviewed
• [Journal Article] An IL-1 cytokine member, IL-33, induces human basophil activation via its ST2 receptor (2008)
  • Author(s): Suzukawa M, Iikura M, Koketsu R, Nagase H, Tamura C, Komiya A, Nakae S, Matsushima K, Ohta K. Yamamoto K, Yamaguchi M
  • Journal Title: J Immunol 181 Pages: 5981-5989
  • Peer Reviewed
• [Journal Article] 気管支喘息のモニタリングをめぐって呼気ガス
  • Author(s): 長瀬洋之,大田健
  • Journal Title: 臨床免疫・アレルギー科 52 Pages: 194-198
• [Presentation] Usefulness of measuring the level of fractional exhaled nitric oxide (FeNO) in various respiratory diseases (2011)
  • Author(s): H Nagase, T Toda, A Kamiyama, Y Nakase, N Sugimoto, H Yoshihara, M Kuramochi, H Tashimo, H Arai, M Yamaguchi, N Suzuki, K Ohta
  • Organizer: 2011 American Academy of Allergy Asthma & Immunol Annual Meeting (AAAAI)
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2011-03-11
• [Presentation] 喘息管理・コントロール状態の評価JGL2009、GINA、EPR3、喘息コントロールテスト(ACT)における喘息コントロール評価の比較 (2010)
  • Author(s): 長瀬洋之,戸田貴子,杉本直也,中瀬裕子,神山麻恵,吉原久直,倉持美知雄,田下浩之,新井秀宜,山口正雄,鈴木直仁,大田健
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] 非喘息性呼吸器疾患における呼気一酸化窒素濃度(FeNO)測定の意義 (2010)
  • Author(s): 戸田貴子,長瀬洋之,神山麻恵,中瀬裕子,杉本直也,鈴川真穂,吉原久直,倉持美知雄,田下浩之,新井秀宜,中野純一,山口正雄,鈴木直仁, 大田健
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] 新型インフルエンザと喘息について多面的に考える成人領域における新型インフルエンザの臨床 (2010)
  • Author(s): 長瀬洋之,古賀一郎,太田康男,大田健
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] 呼気一酸化窒素濃度(FeNO)の疾患特異性の検証 (2010)
  • Author(s): 戸田貴子,長瀬洋之,原麻恵,小河原郁子,鈴川真穂,小島康弘,倉持美知雄,田下浩之,新井秀宜,山口正雄,鈴木直仁,大田健
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-23
• [Presentation] シリカによる慢性肺線維化モデル確立のための長期検討 (2010)
  • Author(s): 原麻恵,長瀬洋之,鈴川真穂,小河原郁子,戸田貴子,小島康弘,倉持美知雄,田下浩之,新井秀宜,山口正雄,鈴木直仁,大田健
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-23
• [Presentation] The relationships between the levels of cytokines and chemokines in exhaled breath condensate and the control status of asthma (2009)
  • Author(s): Hara A, Nagase H, Kojima Y, Suzukawa M, Kuramochi M, Ishida H, Adachi T, Suzuki N, Ohta K
  • Organizer: 2009 American Academy of Allergy Asthma & Immunol Annual Meeting (AAAAI)
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20090300
• [Presentation] The Effect of Diesel Exhaust Particles on Pathogen-associated Molecular Patterns induced Cytokine Generation from Bronchial Epithelial Cells (2009)
  • Author(s): Nagase H,, Takano H, Inoue K, Hara A, Kojima Y, Suzukawa M, Kuramochi M, Ishida H, Adachi T, Suzuki N, Ohta K
  • Organizer: 2009 American Academy of Allergy Asthma & Immunol Annual Meeting (AAAAI)
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20090300
• [Presentation] シンポジウム4ウイルス・細菌・真菌とアレルギーS4-5 Innate Immunityとアレルギー (2009)
  • Author(s): 長瀬洋之,大田健
  • Organizer: 第59回日本アレルギー学会秋季学術大会
  • Place of Presentation: 秋田市
  • Year and Date: 2009-10-31
• [Presentation] International Symposium Session 3 Activation and signaling in inflammatory cells : Expression and Function of Toll-like Receptors in Allergic Inflammatory Cells (2009)
  • Author(s): Nagase H, Hirai K, Yamaguchi M, Ohta K
  • Organizer: 第59回日本アレルギー学会秋季学術大会
  • Place of Presentation: 秋田市
  • Year and Date: 2009-10-31
• [Presentation] Th17系サイトカインが好酸球からのサイトカイン放出に及ぼす影響 (2009)
  • Author(s): 戸田貴子、長瀬洋之、鈴川真穂、原麻恵、小島康弘、倉持美知雄、田下浩之、新井秀宜、山口正雄、大田健
  • Organizer: 第59回日本アレルギー学会秋季学術大会
  • Place of Presentation: 秋田市
  • Year and Date: 2009-10-30
• [Presentation] 遺伝子多型と吸入ステロイド(ICS)反応性との関連 (2009)
  • Author(s): 長瀬洋之、釣木澤尚実、原麻恵、松井永子、近藤直実、秋山一男、大田健
  • Organizer: 第59回日本アレルギー学会秋季学術大会
  • Place of Presentation: 秋田市
  • Year and Date: 2009-10-29
• [Presentation] 喘息患者におけるビーズアレイシステムを用いた呼気凝縮液の検討 (2008)
  • Author(s): 小島康弘,長瀬洋之,原麻恵,矢野智湖,鈴川真穂,石田博文,中野純一,足立哲也,鈴木直仁,大田健
  • Organizer: 第20回日本アレルギー学会春季臨床大会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-13
• [Book] Annual Review呼吸器2008 IGF-1と呼吸器疾患 (2008)
  • Author(s): 長瀬洋之,大田健
  • Total Pages: 277(40-43)
  • Publisher: 中外医学社