Prevention of diabetic nephropathy by MafB

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590944
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: MORITO Naoki University of Tsukuba, 大学院・人間総合科学研究科, 講師 (70463825)
• Co-Investigator(Kenkyū-buntansha): YOH Keigyou 筑波大学, 大学院・人間総合科学研究科, 准教授 (90323302)
• Project Period (FY): 2008 – 2010
• Keywords: 糖尿病性腎症 / 大Maf群蛋白質 / 糸球体上皮細胞

Research Abstract

We have investigated the function of MafB in glomerular epithelial cells. By database surveillance, we have found Maf recognition element (MARE) in the regulatory region of nephrin and podocin gene. Gel mobility shift assays identified that MafB binds to the MARE of the nephrin and podocin gene. Reporter assay also showed MafB could activate the nephrin gene transcription through the MARE. The expression of MafB was decreased in murine diabetic nephropathy models, as well as the expression of nephrin. Diabetic nephropathy might be prevented by overexpression of MafB in glomerular epithelial cells.

Research Products

• [Journal Article] A novel transgenic mouse model of the human multiple myeloma chromosomal translocation t(14;16)(q32;q23). (2011)
  • Author(s): Morito N, Yoh K, Maeda A, Nakano T, Fujita A, Kusakabe M, Hamada M, Kudo T, Yamagata K, Takahashi S
  • Journal Title: Cancer Res. 71(2) Pages: 339-48
  • Peer Reviewed
• [Journal Article] Focal segmental glomerulosclerosis as a complication of hepatitis B virus infection. (2011)
  • Author(s): Sakai K, Morito N, Usui J, Hagiwara M, Hiwatashi A, Fukuda K, Nanmoku T, Toda T, Matsui N, Nagata M, Yamagata K
  • Journal Title: Nephrol Dial Transplant. 26(1) Pages: 371-3
  • Peer Reviewed
• [Journal Article] Role of endogenous retroviruses in murine SLE. (2010)
  • Author(s): Baudino L, Yoshinobu K, Morito N, Santiago-Raber ML, Izui S
  • Journal Title: Autoimmun Rev. 10(1) Pages: 27-34
  • Peer Reviewed
• [Journal Article] MafA-deficient and beta cell-specific MafK-overexpressing hybrid transgenic mice develop human-like severe diabetic nephropathy. (2009)
  • Author(s): Shimohata H, Yoh K, Fujita A, Morito N, Ojima M, Tanaka H, Hirayama K, Kobayashi M, Kudo T, Yamagata K, Takahashi S
  • Journal Title: Biochem Biophys Res Commun. 389 Pages: 235-40
  • Peer Reviewed
• [Journal Article] Selective up-regulation of intact, but not defective env RNAs of endogenous modified polytropic retrovirus by the Sgp3 locus of lupus-prone mice. (2009)
  • Author(s): Yoshinobu K, Baudino L, Santiago-Raber ML, Morito N, Dunand-Sauthier I, Morley BJ, Evans LH, Izui S
  • Journal Title: J Immunol. 182(12) Pages: 8094-103
  • Peer Reviewed
• [Journal Article] Overexpression of T-bet in T cells accelerates autoimmune glomerulonephritis in mice with a dominant Th1 background. (2009)
  • Author(s): Shimohata H, Yamada A, Yoh K, Ishizaki K, Morito N, Yamagata K, Takahashi S
  • Journal Title: J Nephrol. 22 Pages: 123-9
  • Peer Reviewed
• [Journal Article] Secondary membranous glomerulonephritis associated with recipient residual lymphoma cells after allogeneic bone marrow transplantation. (2009)
  • Author(s): Sakai K, Usui J, Kai H, Hagiwara M, Morito N, Saito C, Yoh K, Tsuruoka S, Hirayama K, Aita K, Nagata M, Yamagata K
  • Journal Title: Clin Exp Nephrol. 13 Pages: 174-8
  • Peer Reviewed
• [Journal Article] Hyperglycemia induces oxidative and nitrosative stress and increases renal functional impairment in Nrf2-deficient mice. (2008)
  • Author(s): Yoh K, Hirayama A, Ishizaki K, Yamada A, Takeuchi M, Yamagishi S, Morito N, Nakano T, Ojima M, Shimohata H, Itoh K, Takahashi S, Yamamoto M
  • Journal Title: Genes Cells. 13(11) Pages: 1159-70
  • Peer Reviewed
• [Journal Article] Dissection of genetic mechanisms governing the expression of serum retroviral gp70 implicated in murine lupus nephritis. (2008)
  • Author(s): Baudino L, Yoshinobu K, Morito N, Kikuchi S, Fossati-Jimack L, Morley BJ, Vyse TJ, Hirose S, Jorgensen TN, Tucker RM, Roark CL, Kotzin BL, Evans LH, Izui S
  • Journal Title: J Immunol. 181(4) Pages: 2846-54
  • Peer Reviewed
• [Presentation] New murine model of renal involvement in multiple myeloma (2009)
  • Author(s): Morito N, Yoh K, Fujita A, Yamagata K, Takahashi S
  • Organizer: World Congress of Nephrology
  • Place of Presentation: Milan, Italy
  • Year and Date: 2009-05-23
• [Remarks] ホームページ等
  • URL: http://www.tsukuba-kidney.com/