Establishment of regenerative medicine for the kidney using renal stem cell.

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590946
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Gunma University
• Principal Investigator: MAESHIMA Akito Gunma University, 医学部, 助教 (70431707)
• Project Period (FY): 2008 – 2010
• Keywords: 幹細胞

Research Abstract

Label-retaining cells (LRCs) have been shown to act as the origin of proliferating cells after kidney injury. In the present study, we demonstrated that the number of LRCs as well as the area of the capillary network declined with aging. When renal tubules isolated from rats treated with BrdU label were co-cultured with human umbilical vein endothelial cells (HUVEC), the number of LRCs significantly increased compared to tubules cultured without HUVEC, suggesting that the size of LRC pool might be regulated by the surrounding peritubular capillary network.

Research Products

• [Journal Article] Enhancement of In Vitro Human Tubulogenesis by Endothelial Cell-derived Factors : Implications for In Vivo Tubular Regeneration after Injury. (2011)
  • Author(s): Miya M, Maeshima A, Mishima K, Sakurai N, Ikeuchi H, Kuroiwa T, Hiromura K, Yokoo H, Nojima Y
  • Journal Title: Am J Physiol Renal Physiol. (In press)
  • Peer Reviewed
• [Journal Article] Efficacy and safety of tacrolimus for induction therapy in patients with active lupus nephritis. (2011)
  • Author(s): Takahashi S, Hiromura K, Sakurai N, Matsumoto T, Ikeuchi H, Maeshima A, Kaneko Y, Kuroiwa T, Nojima Y
  • Journal Title: Mod Rheumatol. (In press)
  • Peer Reviewed
• [Journal Article] Effects of proteasome inhibitors on rat renal fibrosis in vitro and in vivo. (2011)
  • Author(s): Sakairi T, Hiromura K, Takahashi S, Hamatani H, Takeuchi S, Tomioka M, Maeshima A, Kuroiwa T, Nojima Y
  • Journal Title: Nephrology (Carlton) 16 Pages: 76-86
  • Peer Reviewed
• [Journal Article] Treatment with valsartan stimulates endothelial progenitor cells and renal label-retaining cells in hypertensive rats. (2011)
  • Author(s): Yoshida Y, Fukuda N, Maeshima A, Yamamoto C, Matsumoto T, Ueno T, Nojima Y, Matsumoto K, Soma M
  • Journal Title: J Hypertens 29 Pages: 91-101
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor binding protein-related protein 1 is expressed in rheumatoid synovium and regulates synovial fibroblast proliferation. (2010)
  • Author(s): Sakurai N, Kuroiwa T, Kayakabe K, Matsumoto T, Maeshima A, Hiromura K, Nojima Y
  • Journal Title: Mod Rheumatol 21 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Nestin is a novel marker for renal tubulointerstitial injury in immunoglobulin A nephropathy. (2010)
  • Author(s): Tomioka M, Hiromura K, Sakairi T, Takeuchi S, Maeshima A, Kaneko Y, Kuroiwa T, Takeuchi T, Nojima Y
  • Journal Title: Nephrology (Carlton) 15 Pages: 568-74
  • Peer Reviewed
• [Journal Article] The immunosuppressive drug mizoribine directly prevents podocyte injury in puromycin aminonucleoside nephrosis. (2010)
  • Author(s): Takeuchi S, Hiromura K, Tomioka M, Takahashi S, Sakairi T, Maeshima A, Kaneko Y, Kuroiwa T, Nojima Y
  • Journal Title: Nephron Exp Nephrol 116 Pages: e3-10
  • Peer Reviewed
• [Journal Article] Fluvastatin prevents podocyte injury in a murine model of HIV-associated nephropathy. (2009)
  • Author(s): Sakurai N, Kuroiwa T, Ikeuchi H, Hiramatsu N, Takeuchi S, Tomioka M, Shigehara T, Maeshima A, Kaneko Y, Hiromura K, Kopp JB, Nojima Y
  • Journal Title: Nephrol Dial Transplant. 24 Pages: 2378-83
  • Peer Reviewed
• [Journal Article] 尿細管再生に関する最近の知見 (2009)
  • Author(s): 前嶋明人
  • Journal Title: Annual Review腎臓 2009 Pages: 43-49
• [Journal Article] Expression of IL-19 and its receptors in RA : potential role for synovial hyperplasia formation. (2008)
  • Author(s): Sakurai N, Kuroiwa T, Ikeuchi H, Hiramatsu N, Maeshima A, Kaneko Y, Hiromura K, Nojima Y
  • Journal Title: Rheumatology (Oxford) 47 Pages: 815-20
  • Peer Reviewed
• [Journal Article] Revised classification of lupus nephritis is valuable in predicting renal outcome with an indication of the proportion of glomeruli affected by chronic lesions. (2008)
  • Author(s): Hiramatsu N, Kuroiwa T, Ikeuchi H, Maeshima A, Kaneko Y, Hiromura K, Ueki K, Nojima Y
  • Journal Title: Rheumatology (Oxford) 47 Pages: 702-7
  • Peer Reviewed
• [Journal Article] 尿細管の再生療法への道 (2008)
  • Author(s): 前嶋明人
  • Journal Title: 医学のあゆみ 225巻4号 Pages: 317-320
• [Journal Article] 尿細管細胞の再生機構 (2008)
  • Author(s): 宮政明、前嶋明人
  • Journal Title: Nephrology Frontier 7巻2号 Pages: 148-152
• [Journal Article] IgA腎症に対するステロイドパルス療法の有効性腎機能低下症例における検討 (2008)
  • Author(s): 池内秀和、廣村桂樹、坂入徹、山下眞、岡上準、冨澤健史、田村茂生、前嶋明人、金子和光、黒岩卓、植木嘉衛、塚田義人、河合弘進、若松良二、野島美久
  • Journal Title: 日本腎臓学会誌 50巻2号 Pages: 114-121
  • Peer Reviewed
• [Journal Article] 腎再生医学に関する最近の知見 (2008)
  • Author(s): 三島敬一郎、前嶋明人
  • Journal Title: 第92回東京腎生理集談会 腎と透析 64巻3号 Pages: 417-421
• [Presentation] Symposium : Involvement of Renal Stem/Progenitor Cells in the Regeneration Process of the Kidney. (2010)
  • Author(s): 前嶋明人
  • Organizer: 3^<rd> Annual Congress of Regenerative Medicine & Stem Cell
  • Place of Presentation: Shanghai, China
  • Year and Date: 20101205-20101207
• [Presentation] Label-Retaining Cells Which Contribute to Tubular Regeneration after Renal Ischemia Are Not a Specific Minor Cell Population in the Kidney. (2010)
  • Author(s): Maeshima A, Miya M, Mishima K, Sakurai N, Ikeuchi H, Kuroiwa T, Hiromura K, Nojima Y
  • Organizer: 43th American Society of Nephrology
  • Place of Presentation: Denver, USA
  • Year and Date: 20101118-20101121
• [Presentation] Efficacy of Low-Dose Tacrolimus (TAC) in Induction Therapy of Lupus Nephritis (LN). (2010)
  • Author(s): Takahashi S, Hiromura K, Hamatani H, Sakurai N, Ikeuchi H, Maeshima A, Kuroiwa T, Nojima Y
  • Organizer: 43th American Society of Nephrology
  • Place of Presentation: Denver, USA
  • Year and Date: 20101118-20101121
• [Presentation] Inhibition of Epithelial-Mesenchymal Transition (EMT) during Renal Fibrosis by an N-Type Calcium Channel Blocker, Cilnidipine. (2010)
  • Author(s): Mishima K, Maeshima A, Miya M, Sakurai N, Ikeuchi H, Kuroiwa T, Hiromura K, Nojima Y
  • Organizer: 43th American Society of Nephrology
  • Place of Presentation: Denver, USA
  • Year and Date: 20101118-20101121
• [Presentation] The Role of SIRP-alpha (SHPS-1) in Experimental Diabetic Nephropathy. (2010)
  • Author(s): Takahashi S, Hiromura K, Hamatani H, Kato-Tomioka M, Maeshima A, Kuroiwa T, Ohnishi H, Matozaki T, Aoki T, Nojima Y
  • Organizer: 43th American Society of Nephrology
  • Place of Presentation: Denver, USA
  • Year and Date: 20101118-20101121
• [Presentation] シンポジウム「臨床応用へ向けた腎臓の再生」内在する尿細管前駆細胞 (2010)
  • Author(s): 前嶋明人
  • Organizer: 第53回日本腎臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-20100618
• [Presentation] カルシウム拮抗薬のEpithelial-mesenchymal transition(EMT)に対する作用 (2010)
  • Author(s): 三島敬一郎、前嶋明人、宮政明、松本孝之、黒岩卓、廣村桂樹、野島美久
  • Organizer: 第53回日本腎臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-20100618
• [Presentation] SIRP-alphaは糸球体上皮細胞の形態と蛋白尿制御に関与する (2010)
  • Author(s): 富岡麻衣、廣村桂樹、高橋哲史、前嶋明人、黒岩卓、青木武生、高田邦昭、大西浩史、的崎尚、野島美久
  • Organizer: 第53回日本腎臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-20100618
• [Presentation] IgA腎症における尿細管間質のnestin発現と腎予後に関する検討 (2010)
  • Author(s): 富岡麻衣、廣村桂樹、高橋哲史、松本孝之、前嶋明人、黒岩卓、野島美久
  • Organizer: 第53回日本腎臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-20100618
• [Presentation] 血管内皮由来因子による腎尿細管前駆細胞数の制御 (2010)
  • Author(s): 宮政明、前嶋明人、三島敬一郎、松本孝之、黒岩卓、廣村桂樹、野島美久
  • Organizer: 第53回日本腎臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-20100618
• [Presentation] 野島美久腎障害後の尿細管再生における血管内皮由来因子の関与 (2010)
  • Author(s): 宮政明、前嶋明人、三島敬一郎、松本孝之、黒岩卓、廣村桂樹
  • Organizer: 第53回日本腎臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20100616-20100618
• [Presentation] The Role of Endothelial-cell derived Factor(s) in the Regeneration Process of Renal Tubules after Injury. (2010)
  • Author(s): Miya M, Maeshima A, Mishima K, Matsumoto T, Kuroiwa T, Hiromura K, Nojima Y
  • Organizer: ISN Nexus Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100415-20100418
• [Presentation] Reduction of Renal Fibrosis by Calcium Channel Blockers, Cilnidipine and Amlodipine, via Multiple Mechanisms. (2010)
  • Author(s): Mishima K, Maeshima A, Miya M, Matsumoto T, Kuroiwa T, Hiromura K, Nojima Y
  • Organizer: ISN Nexus Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100415-20100418
• [Presentation] The role of SIRP-alpha in podocyte structure and function. (2010)
  • Author(s): Tomioka M, Hiromura K, Takahashi S, Takeuchi S, Maeshima A, Kuroiwa K, Kaneko Y, Saito Y, Ohnishi H, Matozaki T, Aoki T, Takata T, Nojima Y
  • Organizer: ISN Nexus Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100415-20100418
• [Presentation] Tubulointerstitial nestin expression as a predictive marker for renal prognosis in IgA nephropathy. (2010)
  • Author(s): Tomioka M, Hiromura K, Sakairi T, Takeuchi S, Maeshima A, Kuroiwa T, Kaneko Y, Takeuchi T, Nojima Y
  • Organizer: ISN Nexus Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100415-20100418
• [Presentation] SIRP (SHPS-1) Signaling Pathway Is Involved in Podocyte Structure and Function. (2009)
  • Author(s): Tomioka M, Hiromura K, Takahashi S, Takeuchi S, Maeshima A, Kuroiwa K, Kaneko Y, Saito Y, Ohnishi H, Matozaki T, Aoki T, Takata T, Nojima Y
  • Organizer: 42th American Society of Nephrology
  • Place of Presentation: San Diego, USA
  • Year and Date: 20091029-20091101
• [Presentation] Capacity of Tubular Regeneration after Injury Is Reduced in Aging Kidney Due to the Low Number of Renal Progenitor Cells. (2009)
  • Author(s): Miya M, Maeshima A, Mishima K, Matsumoto T, Kuroiwa T, Hiromura K, Nojima Y
  • Organizer: 42th American Society of Nephrology
  • Place of Presentation: San Diego, USA
  • Year and Date: 20091029-20091101
• [Presentation] The Role of Endothelial-Cell Derived Factor(s) in Regeneration Process of Renal Tubules after Injury. (2009)
  • Author(s): Miya M, Maeshima A, Mishima K, Matsumoto T, Kuroiwa T, Hiromura K, Nojima Y
  • Organizer: 42th American Society of Nephrology
  • Place of Presentation: San Diego, USA
  • Year and Date: 20091029-20091101
• [Presentation] 腎臓Label retaining cellに対する組織アンジオテンシンIIの役割 (2009)
  • Author(s): 吉田好徳、福田昇、岡田一義、前嶋明人、野島美久、松本紘一
  • Organizer: 第52回日本腎臓学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20090603-20090605
• [Presentation] 糖尿病性腎症におけるSHPS-1シグナル経路に関する検討 (2009)
  • Author(s): 高橋哲史、廣村桂樹、富岡麻衣、竹内茂、前嶋明人、黒岩卓、金子和光、大西浩史、的崎尚、野島美久
  • Organizer: 第52回日本腎臓学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20090603-20090605
• [Presentation] 腎臓の老化における腎尿細管前駆細胞の関与 (2009)
  • Author(s): 宮政明、前嶋明人、三島敬一郎、山下眞、黒岩卓、金子和光、廣村桂樹、野島美久
  • Organizer: 第52回日本腎臓学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20090603-20090605
• [Presentation] ループス腎炎の寛解導入療法におけるタクロリムスの有用性の検討 (2009)
  • Author(s): 高橋哲史、廣村桂樹、渡辺光治、逸見大造、倉林和隆、山下眞、前嶋明人、金子和光、黒岩卓、野島美久
  • Organizer: 第52回日本腎臓学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20090603-20090605
• [Presentation] 腎硬化症におけるARBの腎幹細胞labele-retaining cellへの作用 (2008)
  • Author(s): 吉田好徳、福田昇、岡田一義、山元智衣、前嶋明人、松本紘一
  • Organizer: 第51回日本腎臓学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20080530-20080601
• [Presentation] 糸球体上皮細胞分泌蛋白のプロテオミクス解析によるinsulin-like growth factor binding protein-related protein 1の同定 (2008)
  • Author(s): 松本孝之、桜井則之、平松範行、前嶋明人、金子和光、黒岩卓、廣村桂樹、Kopp Jeffrey、野島美久
  • Organizer: 第51回日本腎臓学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20080530-20080601
• [Presentation] SHPS-1は糸球体上皮細胞に発現し、障害時の蛋白尿の制御に関与する (2008)
  • Author(s): 富岡麻衣、廣村桂樹、竹内茂、坂入徹、奥沢千絵、岡上準、冨澤健史、前嶋明人、黒岩卓、金子和光、大西浩史、的崎尚、野島美久
  • Organizer: 第51回日本腎臓学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20080530-20080601
• [Book] Daughter Cells : Properties, Characteristics and Stem Cells(Regeneration of the Kidney : Involvement of Intra-and Extra-Renal Stem Cells) (2010)
  • Author(s): Akito Maeshima
  • Total Pages: 197-220
  • Publisher: Novapublishers