Renal regeneration by human Embryonic Stem cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590948
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: HISHIKAWA Keiichi The University of Tokyo, 医学部附属病院, 特任准教授 (50255460)
• Project Period (FY): 2008 – 2010
• Keywords: ES細胞 / 再生医療 / HDAC阻害薬 / 脱メチル化剤

Research Abstract

5-Aza-2'-deoxycytidine (5-Aza) effecectively induced WT-1 in human embryonic stem cells, KhES-1, 2 and 3. Next we transplanted 5-Aza treated KhES-1, 2 and 3 into the kidney capsule of SCID mice. After 6-24 weeks, we found generation of kidney tubular cells, but not endothelial cells and podocytes. Our results demonstrated that 5-Aza promotes differentiation of human ES cells to kidney cells, especially tubular cells.

Research Products

• [Journal Article] Trichostatin a prevents TGF-beta1-induced apoptosis by inhibiting ERK activation in human renal tubular epithelial cells. (2010)
  • Author(s): Yoshikawa M., Hishikawa K., Idei M., Fujita T.
  • Journal Title: Eur J Pharmacol 642 Pages: 28-36
  • Peer Reviewed
• [Journal Article] Histone deacetylase modulates the proinflammatory and -fibrotic changes in tubulointerstitial injury. (2010)
  • Author(s): Marumo T., Hishikawa K., Yoshikawa M., Hirahashi J., Kawachi S., Fujita, T.
  • Journal Title: Am J Physiol Renal Physiol 298 Pages: F133-141
  • Peer Reviewed
• [Journal Article] Comparison of antioxidant activity of cilnidipine and amlodipine. (2009)
  • Author(s): Hishikawa K., Takase O., Idei M., Fujita T.
  • Journal Title: Kidney Int 76 Pages: 230-231
  • Peer Reviewed
• [Journal Article] Mac-1 (CD11b/CD18) links inflammation and thrombosis after glomerular injury. (2009)
  • Author(s): Hirahashi J., Hishikawa K., Kaname S., Tsuboi N., Wang Y., Simon D.I., Stavrakis G., Shimosawa T., Xiao L., Nagahama Y., et al.
  • Journal Title: Circulation 120 Pages: 1255-126
  • Peer Reviewed
• [Journal Article] NF-kappaB-dependent genes induced by proteinuria and identified using DNA microarrays. (2008)
  • Author(s): Takase O., Marumo T., Hishikawa K., Fujita T., Quigg R.J., Hayashi M.
  • Journal Title: Clin Exp Nephrol 12 Pages: 181-188
  • Peer Reviewed
• [Journal Article] Epigenetic regulation of BMP7 in the regenerative response to ischemia. (2008)
  • Author(s): Marumo T., Hishikawa K., Yoshikawa M., Fujita T.
  • Journal Title: J Am Soc Nephrol 19 Pages: 1311-1320
  • Peer Reviewed
• [Journal Article] Isolation and potential existence of side population cells in adult human kidney. (2008)
  • Author(s): Inowa T., Hishikawa K., Takeuchi T., Kitamura T., Fujita T.
  • Journal Title: Int J Urol 15 Pages: 272-274
  • Peer Reviewed
• [Presentation] 腎臓再生をめざしたヒトiPS細胞の3次元培養 (2009)
  • Author(s): 菱川慶一
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-06-03
• [Presentation] ヒトiPS細胞の標準化をめざした合成ハイドロゲル培養による包括遺伝子解析 (2008)
  • Author(s): 菱川慶一、吉川真弘、砂河孝之、油谷浩幸、山中伸弥、藤田敏郎
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Presentation] Epigenetics制御による組織幹細胞を介した腎臓再生の可能性 (2008)
  • Author(s): 菱川慶一、丸茂丈史、吉川真弘、藤田敏郎
  • Organizer: 日本再生医療学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-14
• [Book] 幹細胞の分化誘導と応用 (2009)
  • Author(s): 菱川慶一
  • Total Pages: 83-90
• [Remarks] ホームページ等