IgA腎症患者の扁桃における遺伝子発現

2009 Fiscal Year Annual Research Report

• Project/Area Number: 20590957
• Research Institution: Osaka University
• Principal Investigator: 今井 圓裕 Osaka University, 医学系研究科, 准教授 (00223305)
• Co-Investigator(Kenkyū-buntansha): 長澤 康行 大阪大学, 医学系研究科, 助教 (10379167)
• Keywords: IgA腎症 / マイクロアレイ / APOBEC2 / 遺伝子解析 / 腎臟内科学

Research Abstract

本研究では、IgA腎症の原因が扁桃腺で産生されるIgA1に関連すると仮説を立て、まずIgA腎症患者群の扁桃と、慢性扁桃炎の患者群の扁桃の遺伝子発現の差異をマイクロアレイ法で検討することでIgA腎症発症にかかわる扁桃の遺伝子発現の特徴を明らかすることを目的とする。研究の方法としては、IgA腎症患者の扁桃と、コントロール(慢性扁桃炎など)の扁桃のRNAをプールし、マイクロアレイ法を用いて比較検討する。この両者で、差があった遺伝子について実際に各症例にもどってその差が有意であるかどうかを検証する。さらに、その遺伝子群の中でIgA腎症との関連が示唆されるものについて、扁桃での遺伝子発現等を検討する。IgA腎症の患者において扁桃摘出を行った扁桃と、慢性扁桃炎において扁桃摘出を行った扁桃で遺伝子発現の違いをマイクロアレイ法により検討し、IgA腎症の患者において約2倍以上の上昇が認められた遺伝子発現として12種あり、筋肉由来以外のものを抽出すると以下のものがあった。2倍以上変化のあった遺伝子の、変化量(Fold Change)と遺伝子名を列挙すると3.44倍:FKSG22, 3.28倍:balbindin2、2.12倍:APOBEC2、2.11倍:CEXL11、2.08倍:nebulin、2.07倍:sarolipin、2.08倍fibronectin、1.97倍:Osteopontinであった。このうちAPOBEC 2について、遺伝子発現が、IgA腎症患者で実際に有意に上昇し、扁桃において、胚中心の周囲に発現していることを確認した。これらの結果を論文としてまとめ、Biochem Biophys Res Commun.に発表した。

Research Products

• [Journal Article] Microarray analysis of tonsils in immunoglobulin A nephropathy patients (2010)
  • Author(s): Iio K, Nagasawa Y, Iwatani H, Yamamoto R, Horii A, Okuzaki D, Furumatsu Y, Inohara H, Nojima H, Imai E, Isaka Y, Rakugi H.
  • Journal Title: J Nephrol Feb 6(Epub)
  • Peer Reviewed
• [Journal Article] Kidney repair using stem cells : myth or reality as a therapeutic option? (2010)
  • Author(s): Iwatani H, Imai E.
  • Journal Title: J Nephrol Feb 17(Epub)
  • Peer Reviewed
• [Journal Article] E-cadherin and claudin-4 expression has circadian rhythm in adult rat kidney (2010)
  • Author(s): Yamato M, Ito T, Iwatani H, Yamato M, Imai E, Rakugi H.
  • Journal Title: J Nephrol Jan-Feb ; 23(1) Pages: 102-110
  • Peer Reviewed
• [Journal Article] Specialist care and improved long-term survival of dialysis patients (2010)
  • Author(s): Furumatsu Y, Nagasawa Y, Yamamoto R, Iio K, Iwatani H, Matsui I, Takabatake Y, Kawada N, Shoji T, Imai E, Isaka Y, Rakugi H.
  • Journal Title: Nephrol Dial Transplant Jan 8(Epub)
  • Peer Reviewed
• [Journal Article] Utility of the Japanese GFR estimation equation for evaluating potential donor kidney function (2010)
  • Author(s): Kakuta Y, Okumi M, Ichimaru N, Abe T, Nonomura N, Okuyama A, Kojima Y, Isaka Y, Takahara S, Imai E, Horio M.
  • Journal Title: Clin Exp Nephrol. Feb ; 14(1) Pages: 63-67
  • Peer Reviewed
• [Journal Article] The impact of diabetes mellitus on vitamin D metabolism in predialysis patients (2010)
  • Author(s): Tanaka H, Hamano T, Fujii N, Tomida K, Matsui I, Mikami S, Nagasawa Y, Ito T, Moriyama T, Horio M, Imai E, Isaka Y, Rakugi H
  • Journal Title: Bone Nov ; 45(5) Pages: 949-955
  • Peer Reviewed
• [Journal Article] Clinicopathological study of expression of lymphatic vessels in renal allograft biopsy after treatment for acute rejection (2009)
  • Author(s): Oka K, Namba Y, Ichimaru N, Moriyama T, Kyo M, Kokado Y, Imai E, Takahara S.
  • Journal Title: Transplant Pro Dec ; 41(10) Pages: 4154-4158
  • Peer Reviewed
• [Journal Article] Exercise-induced acute kidney injury with reversible posterior leukoencephalopathy syndrome (2009)
  • Author(s): Kimura T, Iio KI, Imai E, Rakugi H, Isaka Y, Hayashi T.
  • Journal Title: Clin Exp Nephrol. Oct 7(Epub)
  • Peer Reviewed
• [Journal Article] A novel classification for IgA nephropathy (2009)
  • Author(s): Yamamoto R, Imai E
  • Journal Title: Kidney Int. Sep ; 76(5) Pages: 477-480
  • Peer Reviewed
• [Journal Article] Prevalence of chronic kidney disease in the Japanese general population (2009)
  • Author(s): Imai E, Horio M, Watanabe T, Iseki K, Yamagata K, Hara S, Ura N, Kiyohara Y, Moriyama T, Ando Y, Fujimoto S, Konta T, Yokoyama H, Makino H, Hishida A, Matsuo S.
  • Journal Title: Clin Exp Nephrol. Dec ; 13(6) Pages: 621-630
  • Peer Reviewed
• [Journal Article] Quiz page June 2009 : worsening kidney function with a history of urolithiasis (2009)
  • Author(s): Kimura T, Yasuda K, Obi Y, Kobayashi K, Kuhara T, Isaka Y, Imai E, Rakugi H, Hayashi T.
  • Journal Title: Am J Kidney Dis. Jun ; 53(6) Pages: A37-39
  • Peer Reviewed
• [Journal Article] The CXCL12 (SDF-1)/CXCR4 axis is essential for the development of renal vasculature (2009)
  • Author(s): Takabatake Y, Sugiyama T, Kohara H, Matsusaka T, Kurihara H, Koni PA, Nagasawa Y, Hamano T, Matsui I, Kawada N, Imai E, Nagasawa T, Rakugi H, Isaka Y.
  • Journal Title: J Am Soc Nephrol. Aug ; 20(8) Pages: 1714-1723
  • Peer Reviewed
• [Journal Article] A candidate gene approach to genetic prognostic factors of IgA nephropathy--a result of Polymorphism Research to Distinguish genetic factors Contributing To progression of IgA Nephropathy (PREDICT-IgAN) (2009)
  • Author(s): Yamamoto R, Nagasawa Y, Shoji T, Inoue K, Uehata T, Kaneko T, Okada T, Yamauchi A, Tsubakihara Y, Imai E, Isaka Y, Rakugi H.
  • Journal Title: Nephrol Dial Transplant Dec ; 24(12) Pages: 3686-3694
  • Peer Reviewed
• [Journal Article] Negative effects of anemia on quality of life and its improvement by complete correction of anemia by administration of recombinant human erythropoietin in posttransplant patients (2009)
  • Author(s): Kawada N, Moriyama T, Ichimaru N, Imamura R, Matsui I, Takabatake Y, Nagasawa Y, Isaka Y, Kojima Y, Kokado Y, Rakugi H, Imai E, Takahara S.
  • Journal Title: Clin Exp Nephrol Aug ; 13(4) Pages: 355-360
  • Peer Reviewed
• [Journal Article] Usefulness of bone resorption markers in hemodialysis patients (2009)
  • Author(s): Hamano T, Tomida K, Mikami S, Matsui I, Fujii N, Imai E, Rakugi H, Isaka Y.
  • Journal Title: Bone. Jul ; 45 Pages: S19-25
  • Peer Reviewed
• [Journal Article] Early plasma exchange for progressive liver failure in recipients of adult-to-adult living-related liver transplants (2009)
  • Author(s): Yamamoto R, Nagasawa Y, Marubashi S, Furumatsu Y, Iwatani H, Iio K, Matsui I, Dono K, Imai E, Monden M, Isaka Y.
  • Journal Title: Blood Purif. 28(1) Pages: 40-46
  • Peer Reviewed
• [Journal Article] Active vitamin D and its analogue, 22-oxacalcitriol, ameliorate puromycin aminonucleoside-induced nephrosis in rats (2009)
  • Author(s): Matsui I, Hamano T, Tomida K, Inoue K, Takabatake Y, Nagasawa Y, Kawada N, Ito T, Kawachi H, Rakugi H, Imai E, Isaka Y.
  • Journal Title: Nephrol Dial Transplant Aug ; 24(8) Pages: 2354-2356
  • Peer Reviewed
• [Journal Article] Fully phosphorylated fetuin-A forms a mineral complex in the serum of rats with adenine-induced renal failure (2009)
  • Author(s): Matsui I, Hamano T, Mikami S, Fujii N, Takabatake Y, Nagasawa Y, Kawada N, Ito T, Rakugi H, Imai E, Isaka Y.
  • Journal Title: Kidney Int. May ; 75(9) Pages: 915-928
  • Peer Reviewed
• [Journal Article] In vivo transfer of small interfering RNA or small hairpin RNA targeting glomeruli (2009)
  • Author(s): Takabatake Y, Isaka Y, Imai E.
  • Journal Title: Methods Mol Biol 466 Pages: 251-263
  • Peer Reviewed
• [Journal Article] Serum 25-hydroxyvitamin D as an independent determinant of 1-84 PTH and bone mineral density in non-diabetic predialysis CKD patients (2009)
  • Author(s): Tomida K, Hamano T, Mikami S, Fujii N, Okada N, Matsui I, Nagasawa Y, Moriyama T, Ito T, Imai E, Isaka Y, Rakugi H.
  • Journal Title: Bone. 44(4) Pages: 678-683
  • Peer Reviewed
• [Presentation] Microarray analysis of upregulated genes in tonsil of IgA nephropathy patients compared with that of tonsillitis (2008)
  • Author(s): Kenichiro Iio, Yasuyuki Nagasawa, Ryohei Yamamoto, Yoshiyuki Furumatsu, Hirotsugu Iwatani, Enyu Imai, Yoshitaka Isaka, Hiromi Rakugi
  • Organizer: Annual Meeting of American Society of Nephrology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 20081106-20081109