Analysis of the inhibiting factor in prion infectivity

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Nagasaki University
• Principal Investigator: KATSUYA Satoh Nagasaki University, 医歯薬学総合研究科, 講師 (70398147)
• Co-Investigator(Kenkyū-buntansha): ICHIRO Miyoshi 名古屋大学, 医学研究科, 教授 (10183972)
• Project Period (FY): 2008 – 2010
• Keywords: プリオン病 / 感染抑制因子 / オステオポンチン

Research Abstract

The normal prion protein is essential to the deposition of the abnormal prion protein. The normal prion protein is present with kidney or the testis with the prion infection mouse, but the abnormality prion protein does not deposit it in the kidney or testis. In other words, unlike the other organs, we show that the normal prion protein is not the place of the conformation of the abnormal prion protein. The possibility that the normal prion protein inhibited the conformation change of the abnormal prion protein was thought about by some kind of mechanisms.
We analyzed and compared the difference of the gene expression in the mRNA with the kidney with the prion infection mouse with the normal mice in prion infection mouse kidney by cDNA substration assay.In this assay, awe think that one over-expression or low-expression in the kidney of the prion infection mouse is the candidate of so-called prion suppressive gene.
We were able to find six candidate genes by this procedure. A gene expression could identify low-expression gene with the kidney and over-expression gene with the brain. We found the gene which one genes were the most suitable to think about in the six candidate genes most and identified genes. The making of the genetic knockout mouse is successful and we started to perform the infected-prion experiment in the knockout mouses.

Research Products

• [Journal Article] Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. (2011)
  • Author(s): Atarashi R, Satoh K, Sano K, et al.
  • Journal Title: Nature medicine. 17 Pages: 175-178
• [Journal Article] Establishment of a standard 14-3-3 protein assay of cerebrospinal fluid as a diagnostic tool for Creutzfeldt-Jakob disease. (2010)
  • Author(s): Satoh K, Tobiume M, Matsui Y, Mutsukura K, Nishida N, Shiga Y, Eguhchi K, Shirabe S, Sata T
  • Journal Title: Lab Invest. 90(11) Pages: 1637-1644
• [Journal Article] Anti-cyclic citrullinated peptide antibody (anti-CCP antibody) is present in the sera of patients with dementia of Alzheimer's type in Asian. (2010)
  • Author(s): Satoh K, Kawakami A, Shirabe S, Tamai M, Sato A, Tsujihata M, Nagasato K, Eguch K
  • Journal Title: Acta Neurol Scand. 121(5) Pages: 338-341
• [Journal Article] Development of an Ultra-Rapid Diagnostic Method Based on Heart-Type Fatty Acid Binding Protein Levels in the CSF of CJD Patients. (2010)
  • Author(s): Matsui Y, Satoh K, Mutsukura K, Watanabe T, Nishida N, Matsuda H, Sugino M, Shirabe S, Eguchi K, Kataoka Y
  • Journal Title: Cell Mol Neurobiol. 30(7) Pages: 991-999
• [Journal Article] The power Doppler ultrasonography score from 24 synovial sites or 6 simplified synovial sites (2010)
  • Author(s): Kawashiri SY, Kawakami A, Iwamoto N, Fujikawa K, Satoh K, Tamai M, Nakamura H, Okada A, Koga T, Yamasaki S, Ida H, Origuchi T, Eguchi K
  • Journal Title: including the metacarpophalangeal joints, reflects the clinical disease activity and level of serum biomarkers in patients with rheumatoid arthritis Rheumatology (Oxford). Epub ahead of print
• [Journal Article] Clinical, serologic and magnetic resonance imaging of 3 cases of inflammatory myopathy with abundant macrophages in the Japanese population. (2010)
  • Author(s): Hara S, Henmi T, Kawakami A, Fujikawa K, Mukae H, Ishimatsu Y, Sakamoto N, Kakugawa T, Kaji K, Fujimoto M, Kuwana M, Tsukada T, Satoh K, Motomura M, Tamai M, Nakamura H, Ida H, Hayashi T, Origuchi T, Eguchi K, Kohno S
  • Journal Title: Rheumatol Int. Epub ahead of print
• [Journal Article] Rheumatoid vasculitis of crural muscles confirmed by muscle biopsy in the absence of inflammatory myopathy : histologic and MRI study. (2010)
  • Author(s): Nakamura H, Okada A, Kawakami A, Yamasaki S, Ida H, Masuda T, Fukuda T, Satoh K, Yoshimura T, Nakashima M, Hayashi T, Eguchi K
  • Journal Title: Rheumatol Int. 30(10) Pages: 1381-1383
• [Journal Article] Early detection of sporadic CJD by diffusion-weighted MRI before the onset of symptoms. (2010)
  • Author(s): Satoh K, Nakaoke R, Nishiura Y, Tsujino A, Motomura M, Yoshimura T, Sasaki K, Shigematsu K, Shirabe S, Eguchi K
  • Journal Title: J Neurol Neurosurg Psychiatry. Epub ahead of print
• [Presentation] Ultrasensitive human prion detection in cerebrospinal fluids by real-time quaking-induced conversion (2010)
  • Author(s): Atarashi R, Satoh K, Sano K, Fuse T, Yamanaka H, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Yamada M, Mizusawa H, Kitamoto T, Amelia McGlade, Steven John Collins, Shirabe S, Katamine S, Nishida N
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 20100908-20100911
• [Presentation] Genetic Prion disease in Japan, An analysis based on the Japanese CJD Surveillance,1999-2009 (2010)
  • Author(s): Ohara M, Sanjo N, Hizume M, Sakai K, Nozaki I, Hamaguchi T, Nakamura Y, Kitamoto T, Shiga Y, Satoh K, Satoh T, Shirabe S, Yamada M, Tateishi J, Mizusawa H
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 20100908-20100911
• [Presentation] Evaluation of a sandwich ELISA for the gamma-isoform of 14-3-3 proteins for laboratory diagnosis of Creutzfeldt-Jakob disease (2010)
  • Author(s): Matsui Y, Satoh K, Miyazaki T, Shirabe S, Atarashi R, Kataoka Y, Nishida N
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 20100908-20100911
• [Presentation] Brain MRI activity and serum biochemical markers for evaluating blood-brain barrier function in Creutzfeldt-Jakob disease (2010)
  • Author(s): Satoh K, Mutsukura K, Atarashi R, Shirabe S, Matsui Y, Kishida H, Kuroiwa Y, Sanjo N, Mizusawa H, Nishida N
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 20100908-20100911
• [Presentation] Ultrasensitive human prion detection in cerebrospinal fluids using real-time quaking-induced conversion (2010)
  • Author(s): Atarashi R, Satoh K, Sano K, Fuse T, Yamanaka H, Yamaguchi N, Ishibashi D, Matsubara T,Nakagaki T, Yamada M, Mizusawa H, Kitamoto T, Amelia McGlade, Steven John Collins, Shirabe S, Katamine S, Nishida N
  • Organizer: Asia-Oceania Symposium on Prion Diseases
  • Place of Presentation: 札幌
  • Year and Date: 20100724-20100725
• [Presentation] Analysis of Biochemical markers and the method of abnormal prion protein in CSF in Human Prion Diseases as Diagnostic markers (2010)
  • Author(s): Satoh K, Atarashi R, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Amelia McGlade, Steven John Collins, Shirabe S, Nishida N
  • Organizer: Asia-Oceania Symposium on Prion Diseases
  • Place of Presentation: 札幌
  • Year and Date: 20100724-20100725
• [Presentation] Analysis of Biochemical markers and the method of abnormal prion protein in CSF in Human Prion Diseases as Diagnostic markers (2010)
  • Author(s): Satoh K, Atarashi R, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Amelia McGlade, Steven John Collins, Shirabe S, Nishida N
  • Organizer: BIT's 1st Annual World Congress of NeuroTalk-2010.
  • Place of Presentation: シンガポール
  • Year and Date: 20100625-20100628
• [Presentation] プリオン病患者脳脊髄液中診断マーカーの比較検討 (2010)
  • Author(s): 佐藤克也、調漸、江口勝美
  • Organizer: 第51回神経学会総会
  • Place of Presentation: 東京
  • Year and Date: 20100520-20100522
• [Presentation] CJDにおける血清バイオマーカーに用いた血液脳関門(BBB)についての検討 (2010)
  • Author(s): 六倉和生、佐藤克也、調漸、岸田日帯、黒岩義之、三條伸夫、水澤英洋
  • Organizer: 第51回神経学会総会
  • Place of Presentation: 東京
  • Year and Date: 20100520-20100522
• [Book] Annual Review神経 (2011)
  • Author(s): 新竜一郎、佐藤克也
  • Total Pages: 120-129
• [Book] 検査所見-脳脊髄液-プリオン病と遅発性ウイルス感染症 (2010)
  • Author(s): 佐藤克也、調漸、江口勝美
  • Total Pages: 88-95
• [Remarks] ホームページ等
  • URL: http://www.med.nagasaki-u.ac.jp/mmi/cmb/index.html
• [Remarks]
  • URL: http://prion.umin.jp/survey/index.html