AQP1 overexpression in the capillary endothelial cells for the enhancement of muscle regeneration and its therapeutic application to myopathies

2010 Fiscal Year Final Research Report

• Project/Area Number: 20591030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Showa University
• Principal Investigator: WAKAYAMA Yoshihiro Showa University, 医学部, 教授 (40138467)
• Co-Investigator(Kenkyū-buntansha): ARATA Satoru 昭和大学, 遺伝子組換え実験室, 准教授 (20159502)
• Co-Investigator(Renkei-kenkyūsha): JIMI Takahiro 昭和大学, 医学部, 兼任講師 (30196654) ; INOUE Masahiko 昭和大学, 医学部, 兼任講師 (50286770) ; SHIBUYA Seiji 昭和大学, 医学部, 兼任講師 (80167444)
• Project Period (FY): 2008 – 2010
• Keywords: 臨床神経形態学

Research Abstract

Adenovirus vector with AQP1 (experimental group : EG) or that with Lac Z (control group : CG) was injected into the skeletal muscle necrotic tissue of normal or mdx mouse hind limb, respectively. The necrotic and regenerative skeletal muscle tissues after 3 days of injection showed that numerous blood vessels with anti-AQP1 antibody immunopositive staining were present, although their numbers appeared not to be different between EG and CG. The AQP1 mRNA levels of muscle regenerative tissues after 3 days of injection were not statistically different between two groups both in normal and mdx mice. Furthermore, AQP1 overexpressing transgenic mouse is now under generation by using LNL-AQP1 expression system. The DNA fragments were injected into the ova of 188 mice and finally we obtained one male mouse with transgene.