2010 Fiscal Year Final Research Report
Vasopressin and Aquaporin-2 Water Channel in Impaired Water Excretion
| Project/Area Number |
20591083
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Jichi Medical University |
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Principal Investigator |
ISHIKAWA San-e Jichi Medical University, 医学部, 教授 (70112620)
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| Co-Investigator(Kenkyū-buntansha) |
FUNAYAMA Hiroshi 自治医科大学, 医学部, 講師 (40296116)
NAKAMURA Tomohiro 自治医科大学, 医学部, 助教 (60382933)
SAITO Tomoyuki 自治医科大学, 医学部, 非常勤医員 (90382910)
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| Project Period (FY) |
2008 – 2010
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| Keywords | バソプレシン / アクアポリン2 / 水利尿不全 / 浸透圧 / プロモーター活性 / 転写調節 / 低Na血症 |
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| Research Abstract |
In the present study we demonstrated the roles of arginine vasopressin (AVP) and aquaporin-2 (AQP2) water channel in impaired water excretion. In the in vitro study the AQP2 promoter assay clarified the presence of tonicity-response element (TonE) in the AQP2 5'-flanking region (-570～-560 bp) as well as cAMP-response element (CRE) for activating AQP2 transcription. An exposure of cells to hypotonic solution diminished the cAMP-activated AQP2 promoter activity. In the in vivo study the expression of AQP2 mRNA and protein were increased in concert with an increase in plasma AVP in the glucocorticoid-deficient rats. The phenomenon was much manifest in the elder rats with glucocorticoid deficiency. The enhanced expressions of AQP2 mRNA and protein were normalized in the rats after glucocorticoid replacement. Varying levels of serum Na were found in the patients with acute infectious diseases. The group of hyponatremia had an elevation of plasma AVP despite of no reduction in circulatory blood volume. The elevated plasma IL-1β could centrally stimulate AVP release, and produce impaired water excretion and hyponatremia. There was 20.9% of the patients with acute myocardial infarction having hyponatremia within 72 hours after the onset. Plasma AVP was significantly increased in the hyponatremic patients compared with that in the normonatremic ones. There was poor short- and long-term prognosis in the patients with hyponatremia.
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[Presentation] Regulation of arginine vasopressin release by body homeostasis and interleukin-6 in subjects with acute infectious diseases.2008
Author(s)
佐々木正美, 吉田昌史, 林智子, 河野瑞穂, 村田美保, 斉藤智之, 生駒亜希, 豊島秀男, 川上正舒, 石川三衛
Organizer
40^<th> Annual Meeting of American Society of Nephrology J Am Soc Nephrol 19: 579A, 2008
Place of Presentation
Philadelphia, PA
Year and Date
20081106-20081109
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[Presentation] Regulation of body fluid homeostasis by arginine vasopressin in subjects with acute infectious diseases.2008
Author(s)
佐々木正美, 吉田昌史, 林智子, 河野瑞穂, 村田美保, 斉藤智之, 生駒亜希, 豊島秀男, 川上正舒, 石川三衛
Organizer
90^<th> Annual Meeting of The Endocrine Society
Place of Presentation
San Francisco, CA.
Year and Date
20080615-20080618
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