2010 Fiscal Year Final Research Report
Analysis of inductive effect of mPOU on PRL cell differentiation for future application for pituitary tumor treatment
| Project/Area Number |
20591095
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Kobe University |
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Principal Investigator |
OKIMURA Yasuhiko Kobe University, 家政学部, 教授 (30204100)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Yutaka 神戸大学, 医学部附属病院, 講師 (70301281)
IIDA Keiji 神戸大学, 大学院・医学研究科, 助教 (80324911)
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| Research Collaborator |
IGUCHI Genzo 神戸大学, 大学院・医学研究科, 助教
TODA Keizou 神戸大学, 保健学研究科, 大学院生
YAMAMOTO Daisuke 神戸大学, 保健学研究科, 大学院生
IKESHITA Nobuko 神戸大学, 保健学研究科, 大学院生
SHIBAHARA Masami 神戸大学, 保健学研究科, 大学院生
SUGIYAMA Yuka 神戸大学, 保健学研究科, 大学院生
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| Project Period (FY) |
2008 – 2010
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| Keywords | 下垂体腫瘍 / プロラクチン / 発生分化 / 転写因子 / 遺伝子 |
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| Research Abstract |
Prolactinoma is a treatable pituitary tumor that responds to dopamine agonists. Induction for PRL cell differentiation may be related to PRL and D2 receptor gene expression and resulted in treatable tumors. In the present study, to clarify the mechanism of PRL gene expression, we searched brain cDNA library and found that mPOU bound to PRL gene and increased PRL reporter gene expression. mPOU also stimulated Pit-1 reporter gene expression. RNA interference for mPOU resulted in a decrease in PRL content and Pit-1 mRNA levels in GH3 cells. Chromatin immuno-precipitation assay showed that mPOU bound to PRL and Pit-1 gene. Taken together, these results suggested that mPOU was a stimulating factor for PRL gene expression directly and indirectly via enhancement of Pit-1 gene expression.
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