2010 Fiscal Year Final Research Report
Analyses of tissue-specific action of SERMs using real-time imaging techniques
| Project/Area Number |
20591098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Kyushu University |
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Principal Investigator |
KAWATE Hisaya Kyushu University, 大学病院, 助教 (20336027)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAYANAGI Ryoichi 九州大学, 大学院・医学研究院, 教授 (30154917)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 核内受容体 / ホルモン / 共焦点顕微鏡 / GFP / 乳癌 |
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| Research Abstract |
To clarify the molecular mechanism underlying the tissue specificity of SERMs, we examined the intracellular localization of ERα using a green fluorescent protein (GFP)-tagged protein in culture cells from various tissues. Although ERα formed intranuclear foci in the presence of estradiol (E2), RLX translocated ER_into the nucleoli in breast cancer cell lines. This phenomenon was not observed in cells from other tissues. Immunofluorescence staining revealed that endogenous ERα was also translocated into the nucleoli in the presence of RLX. Mutation analyses demonstrate that helix 12 of ERα is essential to the nucleolar translocation of ERα. These results suggest that translocation of ERα into the nucleoli is RLX-specific and is a key event for RLX-induced growth repression of mammary gland cells.
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